biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 43:347-351, 2000 | DOI: 10.1023/A:1026755913034

Detection of Somaclonal Variations in Tissue Culture-Derived Date Palm Plants Using Isoenzyme Analysis and RAPD Fingerprints

M.M. Saker1,*, S.A. Bekheet1, H.S. Taha1, A.S. Fahmy2, H.A. Moursy1
1 Plant Cell and Tissue Culture Department, National Research Center, Cairo, Egypt e-mail
2 Molecular Biology Department, National Research Center, Cairo, Egypt

Isoenzyme analysis and activities of peroxidase (PER), polyphenol oxidase (POD) and glutamate oxaloacetate (GOT) and randomly amplified polymorphic DNA (RAPD) fingerprints were used to analyze somaclonal variations in tissue culture-derived date palm plants. The frequency of somaclonal variations was found to be age dependent. Similar isoenzyme patterns for PER and GOT were detected in all analyzed plants. However, variations in activities of the three enzymes studied and in POD isoenzymes were detected. RAPD analysis showed genetic variations in approximately 4 % of the analyzed plants (70 regenerants). The genetic variations were only detected in 6- and 12-months-old cultures. It was observed also that all morphologically abnormal shoots showed genetic variations at the molecular level.

Keywords: biochemical markers; DNA fingerprints; micropropagation; Phoenix dactylifera
Subjects: date palm, tissue culture, somaclonal variations; DNA fingerprints, tissue culture, somaclonal variations; in vitro culture, date palm, tissue culture, somaclonal variations; in vitro culture, micropropagation, somaclonal variations, isoenzyme analysis; in vitro culture, random amplified polymorphic DNA, RAPD, fingerprints, somaclonal variations; in vitro culture, tissue culture, somaclonal variations, isoenzyme analysis; micropropagation, tissue culture, somaclonal variations, isoenzyme analysis; Phoenix dactylifera; random amplified polymorphic DNA, RAPD fingerprints, somaclonal variations; somaclonal variation, isoenzyme analysis

Published: September 1, 2000  Show citation

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Saker, M.M., Bekheet, S.A., Taha, H.S., Fahmy, A.S., & Moursy, H.A. (2000). Detection of Somaclonal Variations in Tissue Culture-Derived Date Palm Plants Using Isoenzyme Analysis and RAPD Fingerprints. Biologia plantarum, 43(3), 347-351. doi: 10.1023/A:1026755913034
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