biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:107-112, 2003 | DOI: 10.1023/A:1022374520121

The Effect of Pseudomonas fluorescens and Fusarium oxysporum f.sp. cubense on Induction of Defense Enzymes and Phenolics in Banana

R. Thangavelu1, A. Palaniswami1, S. Doraiswamy1, R. Velazhahan1
1 Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore-, Tamil Nadu, India

The effect of Pseudomonas fluorescens treatment and Fusarium oxysporum f. sp. cubense inoculation on induction of phenylalanine ammonia-lyase (PAL), peroxidase (POX), chitinase, β-1,3-glucanase and accumulation of phenolics in banana (Musa sp.) was studied. When banana roots were treated with P. fluorescens strain Pf10, a two-fold increase in phenolic content in leaf tissues was recorded 3 - 6 d after treatment. Challenge inoculation with F. oxysporum, the wilt pathogen, steeply increased the phenolic content in P. fluorescens-treated banana plants. Significant increase in POX activity was detected 6 - 9 d after P. fluorescens treatment. PAL, chitinase and β-1,3-glucanase activities increased significantly from 3 d after P. fluorescens treatment and reached the maximum 6 d after treatment. Challenge inoculation with F. oxysporum further increased the enzyme activities. These results suggest that the enhanced activities of defense enzymes and elevated content of phenolics may contribute to bioprotection of banana plants against F. oxysporum.

Keywords: fluorescent pseudomonad; Fusarium wilt; induced resistance; lytic enzymes; Musa sp.

Prepublished online: March 1, 2003; Published: July 1, 2003  Show citation

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Thangavelu, R., Palaniswami, A., Doraiswamy, S., & Velazhahan, R. (2003). The Effect of Pseudomonas fluorescens and Fusarium oxysporum f.sp. cubense on Induction of Defense Enzymes and Phenolics in Banana. Biologia plantarum, 46(1), 107-112. doi: 10.1023/A:1022374520121
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