biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 48:339-344, 2004 | DOI: 10.1023/B:BIOP.0000041084.77832.11

Micropropagation of an Endangered Orchid Anoectochilus formosanus

N.V. Ket1, E.J. Hahn1, S.Y. Park1, D. Chakrabarty1, K.Y. Paek1
1 Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheong-ju, Korea

A rapid and efficient procedure is outlined for in vitro clonal propagation of an elite cultivar of jewel orchid (Anoectochilus formosanus). Multiple shoot proliferation was induced in shoot tip explants on Hyponex (H3) media supplemented with 1 mg dm-3 benzyladenine or 1 - 2 mg dm-3 thidiazuron (TDZ). Addition of activated charcoal (1 g dm-3) to the TDZ containing medium promoted multiple shoot formation (11.1 shoots per explant). However, the regenerated shoots had slow growth rate and failed to elongate. This problem was overcome by transferring the shoot clumps to a hormone free H3 medium supplemented with 2 % sucrose and 0.5 g dm-3 activated charcoal. Rooting was induced in 100 % of the regenerated shoots in the same media. The plantlets were acclimatized and established in greenhouse.

Keywords: activated charcoal; benzyladenine; sugar; thidiazuron
Subjects: Anoectochilus formosanus; cytokinins; in vitro culture, micropropagation; in vitro culture, nutrient media modification; in vitro culture, sugar sources; medicinal plants; nutrient medium, H3, Hyponex; nutrient medium, KC (Knudson); nutrient medium, Linsmaier and Skoog; nutrient medium, Murashige and Skoog; orchid, micropropagation; thidiazuron

Published: September 1, 2004  Show citation

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Ket, N.V., Hahn, E.J., Park, S.Y., Chakrabarty, D., & Paek, K.Y. (2004). Micropropagation of an Endangered Orchid Anoectochilus formosanus. Biologia plantarum, 48(3), 339-344. doi: 10.1023/B:BIOP.0000041084.77832.11
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    References

    1. Belitsky, I., Bersenev, V.N.A.: Jewel Orchids.-Mag. amer. Orchid Soc. 1999: 33-37, 1999.
    2. Chen, Y.Q., Piluek, C.: Effects of thidiazuron and N6-benzylaminopurine on shoot regeneration of Phalaenopsis.-Plant Growth Regul. 16: 99-101, 1995. Go to original source...
    3. Chow, H.T., Hsieh, W.C., Chang, C.S.: In vitro propagation of Anoectochilus formosanus.- J. Sci. Eng. 19: 155-166, 1982.
    4. Druart, P., Wulf, O.D.: Activated charcoal catalyses sucrose hydrolysis during autoclaving.-Plant Cell Tissue Organ Cult. 32: 97-99, 1993. Go to original source...
    5. Ernst, R.: The use of activated charcoal in asymbiotic seedling culture of Paphiopedilum.-Amer. Orchid Soc. Bull. 43: 35-38, 1974.
    6. Ernst, R.: Studies in asymbiotic culture of orchids.-Amer. Orchid Soc. Bull. 44: 12-18, 1975.
    7. Ernst, R.: Effects of thidiazuron on in vitro propagation of Phalaenopsis and Doritaenopsis (Orchidaceae).-Plant Cell Tissue Organ Cult. 39: 273-275, 1994. Go to original source...
    8. Fridborg, G., Eriksson, T.: Effects of activated charcoal on growth and morphogenesis in cell cultures.-Physiol. Plant. 34: 306-308, 1975. Go to original source...
    9. Huetteman, C.A., Preece, J.E.: Thidiazuron: a potent cytokinin for woody plant tissue culture.-Plant Cell Tissue Organ Cult. 33: 105-119, 1993. Go to original source...
    10. Hyndman, S.E., Hasegawa, P.M., Bressan, R.A.: The role of sucrose and nitrogen in adventitious root formation on cultured rose shoots.-Plant Cell Tissue Organ Cult. 1: 229-238, 1982. Go to original source...
    11. Kano, K.: Studies on the media for orchid seed germination.-Memoirs Fac. Agr. Kagawa Univ. 20: 1-68, 1965.
    12. Klimaszeska, K., Keller, W.A.: High frequency plant generation from thin cell layer explants of Brassica napus.-Plant Cell Tissue Organ Cult. 4: 183-197, 1985. Go to original source...
    13. Knudson, L.: A new nutrient solution for germination of orchid seed.-Amer. Orchid Soc. Bull. 15: 214-217, 1946.
    14. Majada, J.P., Rierra, M.I., Sánchez, R.T.: One step more towards taxane production through enhanced Tax us propagation.-Plant Cell Rep. 19: 825-830, 2000. Go to original source...
    15. Meyer, H.J., Staden, J.V.: In vitro multiplication of Ixia flexuosa.-HortScience 25: 1070-1071, 1988. Go to original source...
    16. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    17. Nayak, N.R., Patnaik, S., Rath, S.P.: Direct shoot regeneration from foliar explants of an epiphytic orchid Acampe praemorsa (Roxb.) Blatter and McCann.-Plant Cell Rep. 16: 583-586, 1997. Go to original source...
    18. Paek, K.Y., Yeung, E.C.: The effects of 1-naphthaleneacetic acid and N6-benzyladenine on the growth of Cymbidium forrestii rhizomes in vitro.-Plant Cell Tissue Organ Cult. 24: 65-71, 1991. Go to original source...
    19. Paek, K.Y., Kwang, H.J.: In vitro growth control of horticultural plants by manipulating water potential of media with sucrose, manitol and agar.-In: Quynh, N.T., Uyen, N.V. (ed.): Adapted Propagation Techniques for Commercial Crops of the Tropics. Pp. 126-136. IFS, Hochi Minh City 1993.
    20. Preece, J.E., Imel, M.R.: Plant regeneration from leaf explants of Rhododendron P.J.M. hybrids.-Scientia Hort. 48:159-170, 1991. Go to original source...
    21. Schnapp, S.R., Preece, J.E.: In vitro growth reduction of tomato and carnation microplant.-Plant Cell Tissue Organ Cult. 6: 3-8, 1986. Go to original source...
    22. Zhao, D.L., Guo, G.Q., Wang, X.Y., Zheng, G.C.: In vitro micropropagation of a medicinal plant species Sophora flavescens.- Biol. Plant. 47: 117-120, 2003/04. Go to original source...

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