biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:367-372, 2006 | DOI: 10.1007/s10535-006-0051-z

Estimation of genetic diversity in varieties of Mucuna pruriens using RAPD

P. Padmesh1,*, J. V. Reji1, M. Jinish Dhar2, S. Seeni1
1 Tropical Botanic Garden and Research Institute, Palode, Kerala, India
2 KSR College of Arts and Science, Salem, Tamil Nadu, India

Genetic diversity was estimated in 13 accessions of the otherwise self pollinated Mucuna pruriens (L.) DC. (velvetbean) comprising varieties pruriens and utilis collected from tropical humid forest using 15 RAPD primers. Similarity index value of 0.68 based on Nei and Li's similarity coefficient indicated high degree of genetic variability. Analysis of various genetic diversity indices like total heterozygosity, Nei's gene diversity, percentage of polymorphic loci, expected and observed number of alleles and Shannon index strongly suggests that variety pruriens is genetically more diverse than variety utilis. Chemical analysis with respect to 3,4-dihydroxy-L-phenylalanine (L-DOPA) content showed uniform distribution. Cluster analysis showed grouping of accessions into two major clusters and tendency of accessions of variety pruriens to group according to their geographical locations. Bootstrap analysis confirmed the robustness of the phenogram. The putative hybrid MMP6 with relatively low similarity value index and low L-DOPA content was promising as food or fodder.

Keywords: Fabaceae; L-DOPA; velvetbean
Subjects: dendrogram, phylogenetic tree, phenogram; genetic diversity, variability; Muccuna pruriens; random amplified polymorphic DNA (RAPD); velved bean

Received: September 10, 2004; Accepted: June 18, 2005; Published: September 1, 2006  Show citation

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Padmesh, P., Reji, J.V., Jinish Dhar, M., & Seeni, S. (2006). Estimation of genetic diversity in varieties of Mucuna pruriens using RAPD. Biologia plantarum, 50(3), 367-372. doi: 10.1007/s10535-006-0051-z
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