biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:519-524, 2006 | DOI: 10.1007/s10535-006-0082-5

Identification of a novel elite genotype for in vitro culture and genetic transformation of cotton

S. Jin1, X. Zhang1,*, Y. Nie1, X. Guo1, S. Liang1, H. Zhu1
1 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei, P.R. China

Hypocotyls of cotton (Gossypium hirsutum L.) cultivars cv. YZ-1, Coker 312 and Coker 201 were inoculated on Murashige and Skoog callus induction medium. YZ-1 exhibited a very high regeneration potential, with 81.9 % of the explants inoculated differentiated into embryogenic callus within 8-10 weeks. During the process of callus maintenance (subculture for 1 to 3 years), the total embryos number in Coker 312 and Coker 201 calli dropped sharply, and the percentage of embryo germination decreased. On the contrary, the callus of YZ-1 consistently maintains a high frequency of plant regeneration after long-time subculture. Transgenic kanamycin-resistant calli of Coker 201 partially lost the ability of somatic embryogenesis and plant regeneration. The stress produced by the transformation procedure slightly affected somatic embryogenesis and plant regeneration of YZ-1, which showed minimum loss of plant regeneration ability.

Keywords: Gossypium hirsutum; regeneration potential; somatic embryogenesis
Subjects: auxins; cultivar and genotype differences; genetic diversity, variability; Gossypium hirsutum; hypocotyl; in vitro culture, callus growth and duration; in vitro culture, embryogenesis, somatic embryogenesis; in vitro culture, regeneration, proliferation, differentiation; neomycin phosphotransferase; nutrient medium, Gamborg B5 medium; nutrient medium, Murashige and Skoog; transgenic plants (cf. also in vitro…)

Received: December 17, 2004; Accepted: June 29, 2005; Published: December 1, 2006  Show citation

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Jin, S., Zhang, X., Nie, Y., Guo, X., Liang, S., & Zhu, H. (2006). Identification of a novel elite genotype for in vitro culture and genetic transformation of cotton. Biologia plantarum, 50(4), 519-524. doi: 10.1007/s10535-006-0082-5
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