biologia plantarum

International journal on Plant Life established by Bohumil Nėmec in 1959

Biologia plantarum 50:531-536, 2006 | DOI: 10.1007/s10535-006-0084-3

Stability of RAPD fingerprints in potato: Effect of source tissue and primers

S. K. Chakrabarti1,*, D. Pattanayak1, D. Sarkar1, V. P. Chimote1, P. S. Naik1
1 Central Potato Research Institute, Division of Crop Improvement, Himachal Pradesh, India

Variations in random amplified polymorphic DNA (RAPD) profiles from leaf, stem, root, and tuber tissues were observed in case of two glasshouse grown potato cultivars using 40 decamer primers suggesting possible danger of cultivar misidentification. Genomic DNA extracted from the above four tissues of four in vitro grown potato cultivars, however, produced more uniform RAPD fingerprints. A significant effect of random primers on fingerprint uniformity was observed in case of both glasshouse and in vitro grown samples. A new concept of stability index for random primers based on homogeneity of RAPD profiles obtained from different tissues of a single plant have been introduced. It is concluded that RAPD analysis of genomic DNA extracted from any tissue of in vitro grown potato plants using 14 selected decamer primers could be used to develop RAPD fingerprints for identification of Indian potato cultivars.

Keywords: DNA amplification; molecular marker; polymerase chain reaction (PCR); Solanum tuberosum ssp. tuberosum
Subjects: cultivar and genotype differences; dendrogram, phylogenetic tree, phenogram; potato; random amplified polymorphic DNA (RAPD); Solanum tuberosum

Received: August 18, 2004; Accepted: August 19, 2005; Published: December 1, 2006  Show citation

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Chakrabarti, S.K., Pattanayak, D., Sarkar, D., Chimote, V.P., & Naik, P.S. (2006). Stability of RAPD fingerprints in potato: Effect of source tissue and primers. Biologia plantarum, 50(4), 531-536. doi: 10.1007/s10535-006-0084-3
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