biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 50:697-700, 2006 | DOI: 10.1007/s10535-006-0109-y

Suppression of phenylalanine ammonia-lyase activity elicited in date palm by Fusarium oxysporum f. sp. albedinis hyphal wall elicitor

C. El Modafar1,*, E. El Boustani2, B. Rahioui1, A. El Meziane1, Z. El Alaoui-Talibi1
1 Laboratory of Biotechnology and Molecular Phytopathology, Faculty of Sciences and Technology of Gueliz, Marrakech, Morocco
2 Laboratory of Nutritional and Pathological Biochemistry, Faculty of Sciences of Semlalia, Marrakech, Morocco

The inoculation of the seedling roots of the resistant (Bousthami Noir) and susceptible (Jihel) date palm (Phoenix dactylifera) cultivars by Fusarium oxysporum f. sp. albedinis induced an increase in phenylalanine ammonia-lyase (PAL) activity. The response of the PAL activity in the resistant cultivar was faster and higher than in the susceptible one. However, the elicitation of the seedlings with the hyphal wall elicitor (HWE) of the pathogen induced identical PAL activity in both cultivars. In the resistant cultivar, the the PAL activity elicited with the HWE was not influenced by the addition of the fungal culture filtrate (FCF) whereas it was suppressed in the susceptible cultivar. This FCF suppressor effect was dose-dependent, not influenced by sodium periodate, whereas it was strongly reduced by the heat (121 °C for 45 min) and pronase E. These results show that differential induction of the defence mechanisms in both cultivars was not related to differences in the induction of the PAL activity, but to the suppression of its elicitation in the susceptible cultivar.

Keywords: Phoenix dactylifera; resistant and susceptible cultivars
Subjects: cultivar and genotype differences; date palm; Fusarium oxysporum; hyphal wall elicitor; phenylalanine ammonia lyase; Phoenix dactylifera

Received: August 17, 2004; Accepted: September 1, 2005; Published: December 1, 2006  Show citation

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El Modafar, C., El Boustani, E., Rahioui, B., El Meziane, A., & El Alaoui-Talibi, Z. (2006). Suppression of phenylalanine ammonia-lyase activity elicited in date palm by Fusarium oxysporum f. sp. albedinis hyphal wall elicitor. Biologia plantarum, 50(4), 697-700. doi: 10.1007/s10535-006-0109-y
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