biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:752-754, 2006 | DOI: 10.1007/s10535-006-0123-0

Effect of carbon dioxide on cell growth and saponin production in suspension cultures of Panax ginseng

N. T. Thanh1, H. N. Murthy1,2, D. M. Pandey1, K. W. Yu1, E. J. Hahn1, K. Y. Paek1,*
1 Research Centre for the Deelopment of Advanced Horticultural Technology, Chungbuk National University, Cheongju, South Korea
2 Department of Botany, Karnatak University, Dharwad, India

The effects of carbon dioxide supply within the range of 1-5 % (along with purified air), on cell culture of Panax ginseng were investigated in a balloon type bubble bioreactor containing 4 dm3 of Murashige and Skoog (MS) medium supplemented with 7.0 mg dm-3 indolebutyric acid, 0.5 mg dm-3 kinetin and 30 g dm-3 sucrose. A 1 % CO2 supply was found beneficial for the production of cell mass; however, increasing CO2 concentration to 2.5 and 5 % decreased the biomass accumulation. CO2 enrichment was not beneficial for saponin production and 1, 2.5, and 5 % CO2 supply resulted in decrease in saponin accumulation up to 11.6, 19.5, and 50.6 %, respectively.

Keywords: bioreactors; ginseng; plant cell culture; secondary metabolites
Subjects: auxins; bioreactor; cell growth; CO2 concentration, elevated, enrichment; in vitro culture, cell suspension cultures; nutrient medium, Murashige and Skoog; Panax ginseng; saponin

Received: January 10, 2005; Accepted: June 2, 2005; Published: December 1, 2006  Show citation

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Thanh, N.T., Murthy, H.N., Pandey, D.M., Yu, K.W., Hahn, E.J., & Paek, K.Y. (2006). Effect of carbon dioxide on cell growth and saponin production in suspension cultures of Panax ginseng. Biologia plantarum, 50(4), 752-754. doi: 10.1007/s10535-006-0123-0
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