biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 51:173-176, 2007 | DOI: 10.1007/s10535-007-0034-8

Agar/galactomannan gels applied to shoot regeneration from tobacco leaves

N. Lucyszyn1, M. Quoirin2, M. M. Homma1, M.-R. Sierakowski1,*
1 Laboratório de Biopolímeros, Departamento de Química, Universidade Federal do Paraná, Curitiba, PR, Brazil
2 Laboratório de Micropropagação Vegetal, Departamento de Botânica, Universidade Federal do Paraná, Curitiba, PR, Brazil

This study concerns the efficacy of partial agar substitution by galactomannans as support in plant regeneration media for Nicotiana tabacum. The production of multiple shoots from leaf-derived callus and their rooting were evaluated. The galactomannans applied were obtained from Cassia fastuosa (cassia) and Cyamopsis tetragonolobus (guar gum - a commercial galactomannan) seeds. The results obtained on media solidified with mixtures of agar/galactomannan (3 g dm-3 each) gels were compared with those on media gelled with a standard concentration of agar (6 g dm-3). The in vitro performance allowed to conclude that the use of galactomannans raised the number of shoots and improved their quality. Furthermore, the length of roots and the size of leaves were significantly higher in the media solidified with agar/guar galactomannan mixtures.

Keywords: alternative gelling agent; Cassia fastuosa; Cyamopsis tetragonolobus; guar gum; Nicotiana tabacum
Subjects: Cassia fastuosa; Cyamopsis tetragonolobus; cytokinins; galactoglucomannan, gelling agent; in vitro culture, regeneration, proliferation, differentiation; Nicotiana tabacum; nutrient medium, Murashige and Skoog (MS); tobacco

Received: May 19, 2005; Accepted: November 25, 2005; Published: March 1, 2007  Show citation

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Lucyszyn, N., Quoirin, M., Homma, M.M., & Sierakowski, M.-R. (2007). Agar/galactomannan gels applied to shoot regeneration from tobacco leaves. Biologia plantarum, 51(1), 173-176. doi: 10.1007/s10535-007-0034-8
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    References

    1. Brown, D.C.W., Leung, D.W.M., Thorpe, T.A.: Osmotic requirement for shoot formation in tobacco callus.-Physiol. Plant. 46: 36-41, 1979. Go to original source...
    2. Dea, I.C.M., Morrison, A.: Chemistry and interactions on seed galactomannans.-Adv. Carbohydr. Chem. Biochem. 31: 241-312, 1975. Go to original source...
    3. Debergh, P.C.: Effects of agar brand and concentration on the tissue culture medium.-Physiol. Plant. 59: 270-276, 1983. Go to original source...
    4. Jain, N., Babbar, S.B.: Gum katira - a cheap gelling agent for plant tissue culture media.-Plant Cell Tissue Organ Cult. 71: 223-229, 2002. Go to original source...
    5. Koda, T., Ichi, T. Yamagishi, H., Yokshikawa, H.: Effects of phytohormones and gelling agents on plant regeneration from protoplasts of red cabbage.-Agr. Biol. Chem. 52: 2337-2340, 1988. Go to original source...
    6. Lima-Nishimura, N., Quoirin, M., Naddaf, Y.G., Wilhelm, H.M., Ribas, L.L.F., Sierakowski, M.-R.: A xyloglucan from seeds of the native Brazilian species Hymenaea courbaril for micropropagation of Marubakaido and Jonagored apples.-Plant Cell Rep. 21: 402-407, 2003. Go to original source...
    7. Lucyszyn, N.: Galactomananas: novas fontes do biopolímero e aplicações na indústria de alimentos. [Galactomannans: new sources of the biopolymer and applications in the food industry.]-Master Thesis. Federal University of Parana, Curitiba 1994. [In Port.]
    8. Maier, H., Anderson, M., Karl, C., Maqnuson, K., Whistler, R.L.: Guar, locust bean, tara and fenugreek gums.-In: Whistler, R.L., Bemiller, J.N. (ed.): Industrial Gums: Polysaccharides and Their Derivatives. 3rd Ed. Pp. 215-218. Academic Press, San Diego-New York-Boston-London-Sydney-Tokyo-Toronto 1993. Go to original source...
    9. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    10. Naik, P.S., Sarkar, D.: Sago: an alternative cheap gelling agent for potato in vitro culture.-Biol. Plant. 44: 293-296, 2001. Go to original source...
    11. Nairn, B.J., Furneaux, R.H., Stevenson, T.T.: Identification of an agar constituent responsible for hydric control in micropropagation of radiata pine.-Plant Cell Tissue Organ Cult. 43: 1-11, 1995. Go to original source...
    12. Owens, L.D., Wozniak, C.A.: Measurement and effects of gel matric potential and expressibility on production of morphogenic callus by cultured sugarbeet leaf discs.-Plant Cell Tissue Organ Cult. 26: 127-133, 1991. Go to original source...
    13. Petkowicz, C.L.O., Ganter, J.L.M.S., Sierakowski, M.-R., Reicher, F.: Galactomannans and arabinans from seeds of Caesalpiniaceae.-Phytochemistry 49: 737-743, 1998. Go to original source...
    14. Podwyszynska, M., Olszewski, T.: Influence of gelling agents on shoot multiplication and uptake of macroelements by in vitro culture of rose, cordyline and homalomena.-Scientia Hort. 64: 77-84, 1995. Go to original source...
    15. Reid, G.J.S.: Galactomannans.-In: Dey, P.M., Dixon, R.A. (ed.): Biochemistry of Storage Carbohydrates. Pp. 265-286. Academic Press, San Diego-New York-Boston-London-Sydney-Tokyo-Toronto 1985.
    16. Romberger, J.A., Tabor, C.A.: The Picea abies shoot meristem in culture I. Agar and autoclaving effects.-Amer. J. Bot. 58: 131-140, 1971. Go to original source...
    17. Scholten, H.J., Pierik, R.L.M.: Agar as a gelling agent: chemical and physical analysis.-Plant Cell Rep. 17: 230-235, 1998. Go to original source...
    18. Smýkalová, I., Ortová, M., Lipavská, H., Patzak, J.: Efficient in vitro micropropagation and regeneration of Humulus lupulus on low sugar, starch-gelrite media.-Biol. Plant. 44: 7-12, 2001. Go to original source...
    19. Stolz, L.P.: Agar restriction of the growth of excised mature Iris embryos.-J. amer. Soc. hort. Sci. 96: 618-684, 1971. Go to original source...
    20. Zimmerman, R.H., Bhardwaj, S.V., Fordham, I.M.: Use of starch-gelled medium for tissue of some fruit crops.-Plant Cell Tissue Organ Cult. 43: 207-213, 1995. Go to original source...

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