biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 51:223-228, 2007 | DOI: 10.1007/s10535-007-0045-5

Embryogenesis and plant regeneration from unpollinated ovary culture of Psoralea corylifolia

S. Chand1, A. K. Sahrawat1,*
1 Plant Tissue Culture and Genetics Research Group, School of Life Sciences, Devi Ahilya University, Indore, India

Embryogenesis and plant regeneration was achieved from callus cultures derived from unpollinated ovaries of Psoralea corylifolia L. Callus was initiated from unpollinated ovaries on Murashige and Skoog (MS) medium supplemented with 2.2 µM N 6-benzyladenine (BA) and various concentrations of α-naphthaleneacetic acid (NAA (2.7 to 10.7 µM) or 2,4-dichlorophenoxyacetic acid (2,4-D (2.3 to 9 µM) alone or in combination. Highly organized embryogenic callus induction, embryo development, proliferation and maturation were achieved on transfer of callus clumps to MS medium supplemented with NAA (0.27 µM) or 2,4-D (0.23 µM) alone or in combination with BA (2.2 to 8.8 µM). Addition of abscisic acid (ABA) (0.95 to 5.8 µM) to the medium enhanced average numbers of cotyledonary stage embryos, the maximum number (34.6 ± 0.7) being obtained on MS medium containing 0.27 µM NAA, 2.2 µM BA and 3.8 µM ABA. Embryos germinated on MS medium supplemented with BA (0 to 8.8 µM). MS medium containing gibberellic acid (GA3 (0.29 to 5.8 µM) enhanced embryo germination frequency, the highest frequency (66.7 %) occurring on MS medium containing 2.2 µM BA and 4.3 µM GA3. Effect of several concentrations (3.0 to 6.0 %) of sucrose or maltose was also observed on germination of embryos. MS medium enriched with maltose supported high frequency of embryo germination.

Keywords: abscisic acid; auxins; cytokinins; gibberellic acid; gynogenic haploid; maltose; sucrose
Subjects: abscisic acid (ABA); auxins; cytokinins; gibberellic acid, gibberellin; in vitro culture, embryogenesis, somatic embryogenesis; in vitro culture, regeneration, proliferation, differentiation; maltose; nutrient medium, Murashige and Skoog (MS); ploidy; Psoralea corylifolia; sucrose

Received: June 6, 2005; Accepted: March 25, 2006; Published: June 1, 2007  Show citation

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Chand, S., & Sahrawat, A.K. (2007). Embryogenesis and plant regeneration from unpollinated ovary culture of Psoralea corylifolia. Biologia plantarum, 51(2), 223-228. doi: 10.1007/s10535-007-0045-5
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    References

    1. Alan, A.R., Mutschler, M.A., Brants, A., Cobb, E.D., Earle, E.D.: Production of gynogenic plants from hybrids of Allium cepa L. and A. roylei Stearn.-Plant Sci. 165: 1201-1211, 2003. Go to original source...
    2. Attree, S.M., Fowke, L.C.: Embryogeny of gymnosperms: advances in synthetic seed technology of conifers.-Plant Cell Tissue Organ Cult. 35: 1-35, 1993. Go to original source...
    3. Bajaj, Y.P.S.: In vitro production of haploids and their use in cell genetics and plant breeding.-In: Bajaj, Y.P.S. (ed.): Biotechnology in Agriculture and Forestry: Haploids in Crop Improvement I. Vol. 12. Pp. 3-44. Springer-Verlag, Berlin 1990. Go to original source...
    4. Cappadocia, M., Vieth, J.: Gerbera jamesonii H. Bolus ex Hook: In vitro production of haploids.-In: Bajaj, Y.P.S. (ed.): Biotechnology in Agriculture and Forestry: Haploids in Crop Improvement I. Vol. 12. Pp. 417-427. Springer-Verlag, Berlin 1990. Go to original source...
    5. Castillo, A.M., Cistué, L.: Production of gynogenic haploids of Hordeum vulgare L.-Plant Cell Rep. 12: 139-143, 1993. Go to original source...
    6. Chaleff, R.S., Stolarz, A.: Factors influencing the frequency of callus formation among cultured rice (Oryza sativa L.) anthers.-Physiol. Plant. 51: 201-206, 1981, 1981. Go to original source...
    7. Chand, S., Basu, P.: Embryogenesis and plant regeneration from callus cultures derived from unpollinated ovaries of Hyoscyamus muticus L.-Plant Cell Rep. 17: 302-305, 1998. Go to original source...
    8. Fehér, A., Pasternak, T.P., Dudits, D.: Transition of somatic plant cells to an embryogenic state.-Plant Cell Tissue Organ Cult. 74: 201-228, 2003. Go to original source...
    9. Gu, Z.P., Cheng, K.C.: In vitro induction of haploid plantlets from unpollinated young ovaries of lily and its embryological observations.-Acta bot. sin. 25: 24-28, 1983.
    10. Hu, H., Yang, H.Y.: Haploids of Higher Plants in Vitro.-China Academic Publishers, Beijing/Springer-Verlag, Berlin 1986.
    11. Lakshmi Sita, G.: Gynogenic haploids in vitro.-In: Jain, S.M., Sopory, S.K., Veilleux, R.E. (ed.): In vitro Haploid Production in Higher Plants Vol. 5. Pp. 175-193. Kluwer Academic Publishers, Dordrecht 1997. Go to original source...
    12. Lecouteux, C.G., Lai, F.-M., McKersie, B.D.: Maturation of alfalfa (Medicago sativa L.) somatic embryos by abscisic acid, sucrose and chilling stress.-Plant Sci. 94: 207-213, 1993. Go to original source...
    13. Lu, C., Vasil, I.K.: Somatic embryogenesis and plant regeneration from leaf tissues of Panicum maximum Jacq.-Theor. appl. Genet. 59: 275-280, 1981. Go to original source...
    14. Murashige, S., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    15. Nishiwaki, M., Fujino, K., Kodo, Y., Masuda, K., Kikuta, Y.: Somatic embryogenesis induced by the simple application of abscisic acid to carrot (Daucus carota L.) seedlings in culture.-Planta 211: 756-759, 2000. Go to original source...
    16. Nørgaard, J.V. Somatic embryo maturation and plant regeneration in Abies nordmanniana LK.-Plant Sci. 124: 211-221, 1997. Go to original source...
    17. Rangan, T.S.: Ovary, ovule and nucellus culture.-In: Johri, B.M. (ed.): Experimental Embryology of Vascular Plants. Pp. 105-129. Springer-Verlag, Berlin 1982. Go to original source...
    18. San, L.H., Gelebart, P.: Production of gynogenic haploids.-In: Vasil, I.K. (ed.): Cell Culture and Somatic Cell Genetics of Plants. Vol. 3. Pp. 305-322. Academic Press, Orlando 1986. Go to original source...
    19. San Noeum, L.H.: Haploids d'Hordeum vulgare L. par culture in vitro d'ovaires non fécondés.-Ann. Amélior. Plantes 26: 751-754, 1976.
    20. Satyavati, G.V., Gupta, A.K., Tandon, N.: Psoralea Linn. (Fabaceae; Papilionaceae).-In: Satyavati, G.V., Gupta, A.K., Tandon, N. (ed.): Medicinal Plants of India. Vol. 2. P. 600. Indian Council of Medical Research, New Delhi 1987.
    21. Sauer, U., Wilhelm, E.: Somatic embryogenesis from ovaries, developing ovules and immature zygotic embryos, and improved embryo development of Castanea sativa.-Biol. Plant. 49: 1-6, 2005. Go to original source...
    22. Sibi, M.L., Kobaissi, A., Shekafandeh A.: Green haploid plants from unpollinated ovary culture in tetraploid wheat (Triticum durum Defi.).-Euphytica 122: 351-359, 2001. Go to original source...
    23. Strickland, S.G., Nichol, J.W., McCall, C.M., Stuart, D.A.: Effect of carbohydrate source on alfalfa somatic embryogenesis.-Plant Sci. 48: 113-121, 1987. Go to original source...
    24. Thomas, T.D., Bhatnagar, A.K., Razdan, M.K., Bhojwani, S.S.: A reproducible protocol for the production of gynogenic haploids of mulberry, Morus alba L.-Euphytica 110: 169-173, 1999. Go to original source...
    25. Yang, H.Y., Zhou, C.: In vitro induction of haploid plants from unpollinated ovaries and ovules.-Theor. appl. Genet. 63: 97-104, 1982. Go to original source...
    26. Yang, H.Y., Zhou, C. In vitro gynogenesis.-In: Bhojwani, S.S. (ed.): Plant Tissue Culture: Application and Limitations. Pp. 242-258. Elsevier, Amsterdam 1990. Go to original source...

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