biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 51:517-520, 2007 | DOI: 10.1007/s10535-007-0110-0

Post-transcriptional gene silencing is involved in resistance of transgenic papayas to papaya ringspot virus

P. Ruanjan1, S. Kertbundit2, M. Juøíèek3,*
1 Institute of Molecular Biology and Genetics, Mahidol University, Nakhon Pathom, Thailand
2 Department of Plant Science, Faculty of Science, Mahidol University, Payathai, Bangkok, Thailand
3 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague 6, Czech Republic

Transgenic papayas carrying the papaya ringspot virus coat protein gene were inoculated with Papaya ringspot virus. Infection was monitored by evaluating symptoms and by reverse transcription polymerase chain reaction (RT-PCR). Among eight tested transgenic lines, clone G2 was found highly resistant to virus infection during 3 years of testing. Further analysis of this clone revealed complex multicopy transgene insertion with aberrant copies. The suspected post transcriptional gene silencing was confirmed by siRNA detection. While the R0 generation of G2 transgenic papaya was found to be fully resistant to the infection, Papaya ringspot virus was able to break this resistance in subsequent generations by suppressing post-transcriptional gene silencing (PTGS).

Keywords: Carica papaya; reverse transcription PCR
Subjects: Carica papaya; papaya ringspot virus; polymerase chain reaction (PCR); transgenic plants

Received: April 19, 2005; Accepted: February 17, 2006; Published: September 1, 2007  Show citation

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Ruanjan, P., Kertbundit, S., & Juøíèek, M. (2007). Post-transcriptional gene silencing is involved in resistance of transgenic papayas to papaya ringspot virus. Biologia plantarum, 51(3), 517-520. doi: 10.1007/s10535-007-0110-0
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    References

    1. Anandalakshmi, R., Pruss, G.J., Ge, X., Marathe, R., Mallory, A.C., Smith, T.H., Vance, V.B.: A viral suppressor of gene silencing in plants.-Proc. nat. Acad. Sci. USA 95: 13079-13084, 1998. Go to original source...
    2. Bau, H.-J., Cheng, Y.-H., Yu, T.-A., Yang, J.-S., Yeh S.-D.: Broad-spectrum resistance to different geographic strains of Papaya ringspot virus in coat protein gene transgenic papaya.-Phytopathology 93: 112-120, 2003. Go to original source...
    3. Chiang, C.H., Wang, J.J., Jan, F.J., Yeh, S.D., Gonsalves, D.: Comparative reactions of recombinant papaya ringspot viruses with chimeric coat protein (CP) genes and wild-type viruses on CP-transgenic papaya.-J. gen. Virol. 82: 2827-2836, 2001. Go to original source...
    4. Dehio, C., Schell, J.: Identification of plant genetic loci involved in a posttranscriptional mechanism for meiotically reversible transgene silencing.-Proc. nat. Acad. Sci. USA 91: 5538-5542, 1994. Go to original source...
    5. Dougherty, W.G., Lindbo, J.A., Smith, H.A., Parks, T.D., Swaney, S., Proebsting, W.M.: RNA-mediated virus resistance in transgenic plants: exploitation of a cellular pathway possibly involved in RNA degradation.-Mol. Plant-Microbe Interact. 7: 544-552, 1994. Go to original source...
    6. Guo, H.S., Cervera, M.T., García, J.A.: Plum pox potyvirus resistance associated to transgene silencing that can be stabilized after different number of plant generations.-Gene 206: 263-272, 1998. Go to original source...
    7. Hamilton, A.J., Baulcombe, D.C.: A species of small antisense RNA in posttranscriptional gene silencing in plants.-Science 286: 950-952, 1999. Go to original source...
    8. Kertbundit, S., Pongtanom, N., Ruanjan, P., Chantasingh, D., Tanwanchai, A., Panyim, S., Juøíèek, M.: Resistance of transgenic papaya plants to Papaya ringspot virus, Thai isolate.-Biol. Plant. 51: 333-339, 2007. Go to original source...
    9. Lindbo, J.A., Silva-Rosales, L., Proebsting, W.M., Dougherty, W.G.: Induction of a Highly specific antiviral state in transgenic plants: Implications for regulation of gene expression and virus resistance.-Plant Cell 5: 1749-1759, 1993. Go to original source...
    10. Ling, K., Namba, S., Gonsalves, C., Slightom, J.L., Gonsalves, D.: Protection against detrimental effects of potyvirus infection in transgenic tobacco plants expressing the papaya ringspot virus coat protein gene.-Biotechnology 9: 752-758, 1991. Go to original source...
    11. Lius, S., Manshardt, R.M., Fitch, M.M.M., Slightom, J.L., Sanford, J.C., Gonsalves, D.: Pathogen-derived resistance provides papaya with effective protection against papaya ringspot virus.-Mol. Breed. 3: 161-168, 1997. Go to original source...
    12. Marathe, R., Smith, T.H., Anandalakshmi, R., Bowman, L.H., Fagard, M., Mourrain, P., Vaucheret, H., Vance, V.B.: Plant viral suppressors of post-transcriptional silencing do not suppress transcriptional silencing.-Plant J. 22: 51-59, 2000. Go to original source...
    13. Moreno, M., Bernal, J.J., Jimenez, I., Rodriguez-Cerezo, E.: Resistance in plants transformed with the P1 or P3 gene of tobacco vein mottling potyvirus.-J. gen. Virol. 79: 2819-2827, 1998. Go to original source...
    14. Pang, S.Z., Jan, F.J., Gonsalves, D.: Nontarget DNA sequences reduce the transgene length necessary for RNA-mediated tospovirus resistance in transgenic plants.-Proc. nat. Acad. Sci. USA 94: 8261-8266, 1997. Go to original source...
    15. Rogers, S.G., Bendich, A.J.: Extraction of total cellular DNA from plants, algae and fungi.-In.: Gelvin, S.B., Schilperoort, R.A. (ed.): Plant Molecular Biology Manual, 2nd Ed. Pp. 1-8. Kluwer Academic Press, Dordrecht 1994. Go to original source...
    16. Sanford, J.C., Johnson, S.A.: The concept of parasite-derived resistance: deriving resistance genes from the parasites own genome.-J. theor. Biol. 115: 395-405, 1985. Go to original source...
    17. Szittya, G., Molnar, A., Silhavy, D., Hornyik, C., Burgyan, J.: Short defective interfering RNAs of tombusviruses are not targeted but trigger post-transcriptional gene silencing against their helper virus.-Plant Cell 14: 359-372, 2002. Go to original source...
    18. Tanzer, M.M., Thompson, W.F., Law, M.B., Wernsman, E.A., Uknes, S.: Characterization of post-transcriptionally suppressed transgene expression that confers resistance to tobacco etch virus infection in tobacco.-Plant Cell 9: 1411-1423, 1997. Go to original source...
    19. Tennant, P.F., Gonsalves, C., Ling, K.S., Fitch, M.M.M., Manshardt, R.M., Slightom, J.L., Gonsalves, D.: Differential protection against papaya ringspot virus isolates in coat protein gene transgenic papaya and classically cross protected papaya.-Phytopathology 84: 1359-1366, 1994. Go to original source...
    20. Tripathi, S., Bau, H.-J., Chen, L.-F., Yeh S.-D.: The ability of Papaya ringspot virus strains overcoming the transgenic resistance of papaya conferred by the coat protein gene is not correlated with higher degrees of sequence divergence from the transgene.-Eur. J. Plant Pathol. 110: 871-882, 2004. Go to original source...

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