biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 52:267-274, 2008 | DOI: 10.1007/s10535-008-0057-9

In vivo activity of recombinant human lewis fucosyltransferase III in leaves of Nicotiana tabacum L.

S. Lhernould1, P. Labrousse1, D. Lejolly1, R. Léonard2, M. Carlué1, G. Costa1,*
1 Laboratoire de Chimie des Substances Naturelles, Faculté des Sciences et Techniques, Limoges Cedex, France
2 Institut für Chemie, Universität für Bodenkultur, Vienna, Austria

Fucosylation in plants occurs in glycoproteins and polysaccharides but the function of fucosylation is largely unknown. We aimed to analyze the effects of over-expression of human fucosyltransferase III (hFucT III) on in vivo N-glycan accumulation in tobacco plant leaves and focused on comparing the amount of Lewis a (Lea)-epitope accumulation in transgenic and in wild-type plants. Fucosyltransferase assays, Western blot and mass spectrometry were used to identify, quantify and analyse Lea N-glycans. We found that constitutive overexpression of hFucT III activity had no effect on Lea complex type N-glycans accumulation. Our results suggest that tobacco recombinant hFucT III acts more as a hydrolase than as a transferase.

Keywords: fucose; N-glycans; polysaccharides
Subjects: Agrobacterium strains; N-glycans; Nicotiana tabacum; polysaccharides; proteins; sugars

Received: April 10, 2006; Accepted: January 10, 2007; Published: June 1, 2008  Show citation

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Lhernould, S., Labrousse, P., Lejolly, D., Léonard, R., Carlué, M., & Costa, G. (2008). In vivo activity of recombinant human lewis fucosyltransferase III in leaves of Nicotiana tabacum L. Biologia plantarum, 52(2), 267-274. doi: 10.1007/s10535-008-0057-9
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