biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 52:587-591, 2008 | DOI: 10.1007/s10535-008-0117-1

Role of nitric oxide under saline stress: implications on proline metabolism

A. I. López-Carrión1, R. Castellano1, M. A. Rosales1, J. M. Ruiz1, L. Romero1,*
1 Department of Plant Physiology, Faculty of Sciences, University of Granada, Granada, Spain

The present work is focused on the possible relationship between nitric oxide and the induction of proline in response to salt stress. The plants were subjected to 100 mM NaCl and sodium nitroprusside (SNP; the donor of NO) at different concentrations. The plants showed lower NaCl-induced oxidative stress and proline accumulation after application of low concentrations of SNP together with the NaCl treatment. The reduction in the proline content was related to increased activity of proline dehydrogenase. These results suggest that the NO could be capable of mitigating damage associated with salt stress.

Keywords: ornithine-δ-aminotransferase; proline dehydrogenase; 1-pyrroline-5-carboxylate synthetase; sodium nitroprusside
Subjects: Brassica rapa; Chinese cabbage; malondialdehyde; proline dehydrogenase; salinity, salt stress; sodium nitroprusside

Received: July 26, 2007; Accepted: December 20, 2007; Published: September 1, 2008  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
López-Carrión, A.I., Castellano, R., Rosales, M.A., Ruiz, J.M., & Romero, L. (2008). Role of nitric oxide under saline stress: implications on proline metabolism. Biologia plantarum, 52(3), 587-591. doi: 10.1007/s10535-008-0117-1
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Ashraf, M., Harris, P.J.C.: Potential biochemical indicators of salinity tolerance in plants.-Plant Sci. 166: 3-16, 2004. Go to original source...
    2. Beligni, M.V., Lamattina, L.: Nitric oxide in plants: the history is just beginning.-Plant Cell Environ. 24: 267-278, 2001. Go to original source...
    3. Beligni, M.V., Lamattina, L.: Nitric oxide interferes with plant photo-oxidative stress by detoxifying reactive oxygen species.-Plant Cell Environ. 25: 737-748, 2002. Go to original source...
    4. Bradford, M.M.: A rapid and sensitive method for the quantification of micrograms quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
    5. Charest, C., Phan, C.T.: Cold acclimation of wheat: properties of enzymes involved in proline metabolism.-Physiol. Plant. 80: 159-168, 1990. Go to original source...
    6. Delauney, A.J., Verma, D.P.: Proline biosynthesis and osmoregulation in plants.-Plant J. 4: 215-223, 1993. Go to original source...
    7. Delauney, A.J., Hu, C.-A., Kavi Kishor, P.B., Verma, D.P.S.: Cloning of ornithine-δ-aminotransferase cDNA from Vigna aconitifolia by transcomplementation in Escherichia coli and regulation of proline biosynthesis.-J. biol. Chem. 268: 18673-18678, 1993. Go to original source...
    8. Delledonne, M., Xia, Y., Dixon, R.A., Lamb, C.: Nitric oxide functions as a signal in plant disease resistance.-Nature 394: 585-588, 1998. Go to original source...
    9. Demiral, T., Türkan, I.: Comparative lipid peroxidation, antioxidant defense systems and proline content in roots of two rice cultivars differing in salt tolerance.-Environ. exp. Bot. 53: 247-257, 2005. Go to original source...
    10. Diatloff, E., Rengel, Z.: Compilation of simple spectrophotometric techniques for the determination of elements in nutrient solutions.-J. Plant Nutr. 24: 75-86, 2001. Go to original source...
    11. Fu, J., Huang, B.: Involvement of antioxidant and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress.-Environ. exp. Bot. 45: 105-114, 2001. Go to original source...
    12. Hare, P.D., Cress, W.A.: Metabolic implications of stress-induced proline accumulation in plants.-Plant Growth Regul. 21: 79-102, 1997. Go to original source...
    13. Hmida-Sayari, A., Gargouri-Bouzid, R., Bidani, A., Jaoua, L., Savouré, A., Jaoua, S.: Overexpresion of Δ1-pyrroline-5-carboxylate synthetase increases proline production and confers salt tolerance in transgenic potato plants.-Plant Sci. 169: 746-752, 2005. Go to original source...
    14. Ignarro, L.J., Barry, B.K., Gruetter, D.Y., Edwards, J.C., Ohlstein, E.H., Gruetter, C.A., Baricos, W.H.: Guanylate-cyclase activation by nitroprusside and nitrosoguanidine is related to formation of s-nitrosothiol intermediates.-Biochem. biophys. Res. Commun. 94: 93-100, 1980. Go to original source...
    15. Irigoyen, J.J., Emerich, D.W., Sánchez-Díaz, M.: Water stress induced changes in the concentrations of proline and total soluble sugars in nodulated alfafa (Medicago sativa) plants.-Physiol. Plant. 84: 55-60, 1992. Go to original source...
    16. Juan, M., Rivero, R.M., Romero, L., Ruiz, J.M.: Evaluation of some nutritional and biochemical indicators in selecting salt-resistant tomato cultivars.-Environ. exp. Bot. 54: 193-201, 2005. Go to original source...
    17. Kavi Kishor, P.B., Sangam, S., Amrutha, R.N., Sri Laxmi, P., Naidu, K.R., Rao, K.R.S.S., Rao, S., Reddy, K.J., Theriappan, P., Sreenivasulu, N.: Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: its implications in plant growth and abiotic stress tolerance.-Curr. Sci. 88: 424-438, 2005.
    18. LaRosa, P.C., Rhodes, D., Rhodes, J.C., Bressan, R.A. Csonka, L.N.: Elevated accumulation of proline in NaCl-adapted tobacco cells is not due to altered Δ1-pyrroline-5-carboxylate reductase.-Plant Physiol. 96: 245-250, 1991. Go to original source...
    19. Lutts, S., Majerus, V., Kinet, J.M.: NaCl effects on proline metabolism in rice (Oryza sativa) seedlings.-Physiol. Plant. 105: 450-458, 1999. Go to original source...
    20. Matysik, J., Bhalu, B.A, Mohanty, P.: Molecular mechanisms of quenching of reactive oxygen species by proline under stress in plants.-Curr. Sci. 82: 525-532, 2002.
    21. Minguez-Mosquera, M.I., Jaren-Galen, M., Garrido-Fernández, J.: Lipoxygenase activity during pepper ripening and processing of paprika.-Phytochemistry 32: 1103-1108, 1993. Go to original source...
    22. Mukherje, S.P., Choudhuri, M.A.: Implications of water stress-induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings.-Physiol. Plant. 58: 166-170, 1983. Go to original source...
    23. Paquin, R., Lechasseur, P.: Observations sur une méthode de dosage de la proline libre dans les extraits de plantes.-Can. J. Bot. 57: 1851-1854, 1979. Go to original source...
    24. Parida, A.K., Das, A.B.: Salt tolerance and salinity effects on plants: a review.-Ecotoxicol. Environ. Safe 60: 324-349, 2005. Go to original source...
    25. Rosales, M.A., Ríos, J.J., Castellano, R., López-Carrión, A.I., Romero, L., Ruiz, J.M.: Proline metabolism in cherry tomato exocarp in relation to temperatura and solar radiation.-J. hort. Sci. Biotechnol. (in press).
    26. Sumithra, K., Jutur, P.P., Carmel, B.D., Reddy, A.R.: Salinity-induced changes in two cultivars of Vigna radiata: responses of antioxidative and proline metabolism.-Plant Growth Regul. 50: 11-22, 2006. Go to original source...
    27. Tian, X., Lei, Y.: Nitric oxide treatment alleviates drought stress in wheat seedlings.-Biol. Plant. 50: 775-778, 2006. Go to original source...
    28. Tripathi, S.B., Gurumurthi, K., Panigrahi, A.K., Shaw, B.P.: Salinity induced changes in proline and betaine contents and synthesis in two aquatic macrophytes differing in salt tolerance.-Biol. Plant. 51: 110-115, 2007. Go to original source...
    29. Víteček, J., Wünschová, A., Petřek, J., Adam, V., Kizek, R., Havel, L.: Cell death induced by sodium nitropruside and hydrogen peroxide in tobacco BY-2 cell suspension.-Biol. Plant. 51: 472-749, 2007. Go to original source...
    30. Wolf, B.: A comprehensive system of leaf analysis and its use for diagnosing crop nutrients status.-Commun. Soil Sci. Plant Anal. 13: 1035-1059, 1982. Go to original source...
    31. Zhang, Y., Wang, L., Liu, Y., Zhang, Q., Wei, Q., Zhang, W.: Nitric oxide enhances salt tolerance in maize seedlings through increasing activities of proton-pump and Na+/H+ antiport in the tonoplast.-Planta 224: 545-555, 2006. Go to original source...
    32. Zhao, L., Zhang, F., Guo, J., Yang, Y., Li, B., Zhang, L.: Nitric oxide functions as a signal in salt resistance in the calluses from two ectotypes of reed.-Plant Physiol. 134: 849-857, 2004. Go to original source...

    Return to the content