biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 53:415-421, 2009 | DOI: 10.1007/s10535-009-0080-5

Effect of sucrose application, minerals, and irradiance on the in vitro growth of Cistus incanus seedlings and plantlets

D. Mills1
1 The Jacob Blaustein Institutes for Desert Research, Bergmann Campus, Ben-Gurion University of the Negev, Beer-Sheva, Israel

To study the effect of sucrose on the sink-source relationship in in vitro-grown plants, Cistus incanus seedlings and plantlets were grown horizontally in a two-compartment Petri dish (split dish), with the root system in one compartment and the shoot in the other. Shoots and roots were exposed to different sucrose concentrations (0-30 g dm-3), two irradiance levels (25 and 160 µmol m-2s-1) and the presence or absence of a minimum medium containing minerals and vitamins (M medium). Root and shoot biomass of the seedlings was enhanced by an increase in irradiance when the growth medium was not supplemented with sucrose indicating the role of photosynthesis in biomass production. When sucrose was added to either organ growth was enhanced as well. In the presence of sucrose in the root compartment, sucrose applied to the shoot compartment enhanced growth of both organs under low irradiance, while under high irradiance, sucrose had no further additive effect. In the absence of sucrose in the root compartment, the enhancement of root biomass by sucrose added to the shoot compartment was lower under high irradiance than under low irradiance. The response of Cistus plantlets to sucrose and irradiance differed from that of seedlings, probably reflecting a greater susceptibility of the plantlets to sucrose feedback inhibition on photosynthesis and biomass accumulation. The decrease in root and shoot growth when M medium was added to the shoot compartment and the relatively better growth of these organs when the roots were supplied with minerals and the shoot with sucrose, indicate that growth of the two organs in our experimental set-up was regulated by opposing fluxes of C and nutrients.

Keywords: sink-source relationship; two-compartment Petri dish
Subjects: Cistus incanus; growth and growth analysis; photon flux density; sink - source; sucrose

Received: August 2, 2007; Accepted: February 6, 2008; Published: September 1, 2009  Show citation

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Mills, D. (2009). Effect of sucrose application, minerals, and irradiance on the in vitro growth of Cistus incanus seedlings and plantlets. Biologia plantarum, 53(3), 415-421. doi: 10.1007/s10535-009-0080-5
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