biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:560-564, 2009 | DOI: 10.1007/s10535-009-0101-4

Abscisic acid and auxin accumulation in Catasetum fimbriatum roots growing in vitro with high sucrose and mannitol content

L. E. P. Peres1,*, A. Zsögön1, G. B. Kerbauy2
1 Department of Biological Sciences (LCB), Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ), Universidade de São Paulo, Piracicaba, SP, Brazil
2 Departament of Botany, Institute of Biosciences, Universidade de São Paulo, São Paulo, SP, Brazil

Endogenous contents of indolyl-3-acetic acid (IAA) and abscisic acid (ABA) were quantified in excised roots of Catasetum fimbriatum (Orchidaceae) cultured in vitro on solidified Vacin and Went medium with 1, 2, 4, 6, 8 and 10 % sucrose, as well as 2 % sucrose plus mannitol. Maximum root growth was observed in media with 4 % sucrose and 2 % sucrose plus 2.2 % mannitol, suggesting that a moderate water or osmotic stress promotes orchid root growth. Contents of both ABA and IAA increased in parallel to increasing sucrose concentration and a correlation between root elongation and the ABA/IAA ratio was observed. Incubating isolated C. fimbriatum roots with radiolabeled tryptophan, we showed an accumulation of IAA and its conjugates.

Keywords: hormone interactions; root elongation; osmotic stress
Subjects: abscisic acid (ABA); auxins; Catasetum fimbriatum; in vitro culture, rooting; mannitol; sucrose; sugars; water content

Received: February 19, 2007; Accepted: May 31, 2008; Published: September 1, 2009  Show citation

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Peres, L.E.P., Zsögön, A., & Kerbauy, G.B. (2009). Abscisic acid and auxin accumulation in Catasetum fimbriatum roots growing in vitro with high sucrose and mannitol content. Biologia plantarum, 53(3), 560-564. doi: 10.1007/s10535-009-0101-4
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