biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:565-568, 2009 | DOI: 10.1007/s10535-009-0102-3

Effect of esculetin on activities of pumpkin glutathione S-transferases and growth of pumpkin seedlings

M. D. Hossain1, M. Fujita1,*
1 Department of Plant Sciences, Faculty of Agriculture, Kagawa University, Kagawa, Japan

The effect of esculetin and some related coumarins (coumarin, 7-hydroxycoumarin and scopoletin) on growth of pumpkin (Cucurbita maxima Duch.) seedlings and the activities of pumpkin glutathione S-transferases (GSTs) were investigated. Coumarin and esculetin affected the growth of seedlings. The hypocotyls of affected seedlings became weak and bent at the mid region, roots became very soft with brownish discoloration, and finally seedlings died. Among the compounds tested, only esculetin inhibited CmGSTU3 and CmGSTU2 activities measured with 1-chloro-2,4-dinitrobenzene (CDNB) and at a concentration of 22 µM, it inhibited the activity of CmGSTU3 by 50 %.

Keywords: coumarins; Cucurbita maxima; detoxification; fluorescent substance; inhibitor
Subjects: coumarins; Cucurbita; esculetin; glutathione-S-transferase; pumpkin

Received: July 26, 2007; Accepted: April 25, 2008; Published: September 1, 2009  Show citation

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Hossain, M.D., & Fujita, M. (2009). Effect of esculetin on activities of pumpkin glutathione S-transferases and growth of pumpkin seedlings. Biologia plantarum, 53(3), 565-568. doi: 10.1007/s10535-009-0102-3
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