biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 54:461-470, 2010 | DOI: 10.1007/s10535-010-0082-3

Effect of abiotic stresses on the activity of antioxidative enzymes and contents of phytohormones in wild type and AtCKX2 transgenic tobacco plants

Z. Mýtinová1,2, V. Motyka3, D. Haisel1,2, A. Gaudinová3, Z. Lubovská1,2, N. Wilhelmová2,*
1 Faculty of Science, Charles University, Praha 2, Czech Republic
2 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic
3 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic

The responses of antioxidant enzymes (AOE) ascorbate peroxidase (APX), glutathione reductase (GR), superoxide dismutase (SOD), and catalase (CAT) in soluble protein extracts from leaves and roots of tobacco (Nicotiana tabacum L. cv. Samsun NN) plants to the drought stress, salinity and enhanced zinc concentration were investigated. The studied tobacco included wild-type (WT) and transgenic plants (AtCKX2) harbouring the cytokinin oxidase/dehydrogenase gene under control of 35S promoter from Arabidopsis thaliana (AtCKX2). The transgenic plants exhibited highly enhanced CKX activity and decreased contents of cytokinins and abscisic acid in both leaves and roots, altered phenotype, retarded growth, and postponed senescence onset. Under control conditions, the AtCKX2 plants exhibited noticeably higher activity of GR in leaves and APX and SOD in roots. CAT activity in leaves always decreased upon stresses in WT while increased in AtCKX2 plants. On the contrary, the SOD activity was enhanced in WT but declined in AtCKX2 leaves. In roots, the APX activity prevailingly increased in WT while mainly decreased in AtCKX2 in response to the stresses. Both WT and AtCKX2 leaves as well as roots exhibited elevated abscisic acid content and increased CKX activity under all stresses while endogenous CKs and IAA contents were not much affected by stress treatments in either WT or transgenic plants.

Keywords: abscisic acid; cytokinin; cytokinin oxidase, dehydrogenase; drought; salinity; zinc
Subjects: abscisic acid (ABA); ascorbate peroxidase; carotenoids; catalase; chlorophyll a,b; cytokinin oxidase/dehydrogenase; glutathione reductase; Nicotiana tabacum; salinity; superoxide dismutase (SOD); tobacco; transgenic plants; water stress; xanthophylls; zinc

Received: January 18, 2009; Accepted: November 25, 2009; Published: September 1, 2010  Show citation

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Mýtinová, Z., Motyka, V., Haisel, D., Gaudinová, A., Lubovská, Z., & Wilhelmová, N. (2010). Effect of abiotic stresses on the activity of antioxidative enzymes and contents of phytohormones in wild type and AtCKX2 transgenic tobacco plants. Biologia plantarum, 54(3), 461-470. doi: 10.1007/s10535-010-0082-3
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