biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 54:579-582, 2010 | DOI: 10.1007/s10535-010-0104-1

The influence of sulfur limitation on expression levels of an o-acetylserine (thiol) lyase gene cloned from Vicia sativa

A. U. Novero1,*, R. Ford1
1 Melbourne School of Land and Environment, University of Melbourne, Parkville, Australia

The o-acetylserine (thiol) lyase (OAS-TL) gene is part of the plant sulfate assimilation pathway. The synthesis of cysteine, the first product of the pathway containing organic sulfur, has been previously observed to be dependent on the availability of sulfur and activity of OAS-TL. In this study, the ability of Vicia sativa L. to alter its metabolism to compensate for deficiencies brought about by sulfur stress was utilized to elucidate the functionality of Voas-tl5 (GenBank Accession No. DQ456491), a gene cloned from Vicia sativa. The transcription levels of Voas-tl5 increased in response to sulfur deficiency, indicating that the gene was active in the cysteine biosynthetic pathway.

Keywords: cysteine biosynthesis; sulfate assimilation; vetch
Subjects: o-acetylserine (thiol) lyase; cysteine; proteins; sulphur assimilation; vetch; Vicia sativa

Received: November 26, 2008; Accepted: May 5, 2009; Published: September 1, 2010  Show citation

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Novero, A.U., & Ford, R. (2010). The influence of sulfur limitation on expression levels of an o-acetylserine (thiol) lyase gene cloned from Vicia sativa. Biologia plantarum, 54(3), 579-582. doi: 10.1007/s10535-010-0104-1
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