biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 55:541-544, 2011 | DOI: 10.1007/s10535-011-0121-8

Evaluation of the effect of in vitro stress and competition on tissue culture response of flax

M. Yildiz1,*
1 Department of Field Crops, Faculty of Agriculture, University of Ankara, Diskapi, Ankara, Turkey

This study was carried out to investigate the in vitro competition in tissue culture of three flax (Linum usitatissimum L.) cultivars using different distances among hypocotyl explants cultured. Hypocotyl fresh and dry masses, shoot regeneration percentage, shoot number per hypocotyl, regenerated shoot length and total chlorophyll content were examined during shoot regeneration, while plantlet height, number of roots and length of roots were recorded during rooting. With decreasing distance among explants we observed increased shoot regeneration and rooting till a certain point from where stress initiated and significant decreases in all parameters observed. Explants cultured at distance 1.0 cm were found to be at their optimum.

Keywords: competition; Linum usitatissimum; plantlet establishment

Received: October 19, 2009; Accepted: March 8, 2010; Published: September 1, 2011  Show citation

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Yildiz, M. (2011). Evaluation of the effect of in vitro stress and competition on tissue culture response of flax. Biologia plantarum, 55(3), 541-544. doi: 10.1007/s10535-011-0121-8
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