biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 56:560-565, 2012 | DOI: 10.1007/s10535-012-0070-x

A positive regulatory role of the watermelon ClWRKY70 gene for disease resistance in transgenic Arabidopsis thaliana

S. M. Cho2, E. Y. Kang1, K. H. Min1, Y. K. Lee1, Y. C. Kim1, K. Y. Yang1, K. S. Kim3, Y. S. Choi2, B. H. Cho1,*
1 Department of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea
2 Department of Floriculture, Chunnam Techno College, Jeonnam, Republic of Korea
3 Jangheunggun Mushroom Research Institute, Jeonnam, Republic of Korea

A pathogen-inducible WRKY cDNA was cloned from the leaves of watermelon (Citrullus lanatus) seedlings 24 h after inoculation with Cladosporium cucumerinum. The deduced protein of the gene, designated as ClWRKY70, was classified as a group III WRKY protein based on its single WRKY domain containing a Cys2HisCys zinc-finger motif. Its Arabidopsis thaliana sequence homologue (AtWRKY70) has been described as playing an important role in the plant defense response. ClWRKY70 gene transcripts were highly accumulated in watermelon by salicylic acid treatment, but not by jasmonic acid. By evaluating target gene expression in transgenic Arabidopsis overexpressing the ClWRKY70 gene, it is suggested that the watermelon WRKY gene may play a positive regulatory role in plant resistance against pathogen attack.

Keywords: Botrytis cinerea; Citrullus lanatus; Cladosporium cucumerinum; Erwinia carotovora; jasmonic acid; salicylic acid
Subjects: salicylic acid; jasmonates; WRKY genes; disease resistance; amino acid sequence; gene expression

Received: June 29, 2010; Accepted: May 15, 2011; Published: September 1, 2012  Show citation

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Cho, S.M., Kang, E.Y., Min, K.H., Lee, Y.K., Kim, Y.C., Yang, K.Y., ... Cho, B.H. (2012). A positive regulatory role of the watermelon ClWRKY70 gene for disease resistance in transgenic Arabidopsis thaliana. Biologia plantarum, 56(3), 560-565. doi: 10.1007/s10535-012-0070-x
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