biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 56:607-612, 2012 | DOI: 10.1007/s10535-012-0060-z

Effect of N6-benzyladenine and indole-3-butyric acid on photosynthetic apparatus of Orthosiphon stamineus plants grown in vitro

D. Stoyanova-Koleva1,*, M. Stefanova1, M. Zhiponova2, V. Kapchina-Toteva2
1 Department of Botany, Faculty of Biology, Sofia University "St. Kliment Ohridski", Sofia, Bulgaria
2 Department of Plant Physiology, Faculty of Biology, Sofia University "St. Kliment Ohridski", Sofia, Bulgaria

The leaf structure and chloroplast ultrastructure of kidney tea (Orthosiphon stamineus Benth.) was studied in in vitro culture on standard MS medium supplemented with or without plant growth regulators (PGRs). The cytokinin N6-benzyladenine (BA) negatively affected the structure of the palisade parenchyma and chloroplast ultrastructure and increased the stomatal frequency of the adaxial epidermis. The auxin indole-3-butyric acid (IBA) did not modify the morphology of regenerated leaf tissues as well as the chloroplast ultrastructure. The effect of both PGRs applied in combination was manifested in well-differentiated mesophyll parenchyma, typical chloroplast ultrastructure and increased stomatal frequency on both leaf surfaces. This protocol can be suggested for further ex vitro propagation.

Keywords: auxins; chloroplast ultrastructure; cytokinin; leaf anatomy; medicinal plant; micropropagation
Subjects: in vitro culture; benzyladenine; indolebutyric acid; leaf - epidermis; leaf - mesophyll; stomatal density; chloroplast ultrastructure; starch

Received: July 19, 2011; Accepted: November 2, 2011; Published: December 1, 2012  Show citation

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Stoyanova-Koleva, D., Stefanova, M., Zhiponova, M., & Kapchina-Toteva, V. (2012). Effect of N6-benzyladenine and indole-3-butyric acid on photosynthetic apparatus of Orthosiphon stamineus plants grown in vitro. Biologia plantarum, 56(4), 607-612. doi: 10.1007/s10535-012-0060-z
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