biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 56:729-734, 2012 | DOI: 10.1007/s10535-012-0056-8

Cadmium exclusion a key factor in differential Cd-resistance in Thlaspi arvense ecotypes

S. R. Martin1, M. Llugany1, J. Barceló1, C. Poschenrieder1,*
1 Lab. Fisiología Vegetal, Facultad de Biociencias, Universidad Autónoma de Barcelona, Bellaterra, Spain

Differences in Cd accumulation and Cd tolerance between Thlaspi arvense ecotype Aigues Vives (AV) from a commercial grower in South France and ecotype Jena collected in the polluted urban area of Jena (Germany) were reported here. Ecotype Jena exhibited considerable Cd-tolerance. Shoot and root masses were unaffected and root elongation was even enhanced by exposure to 50 μM Cd. In contrast, growth of ecotype AV was severely affected by this Cd treatment. Ecotype Jena was much more efficient in excluding Cd from both roots and shoots than ecotype AV. Despite the efficient restriction of Cd transport from roots to shoots in Jena, this ecotype maintained high root to shoot transport of Zn and Fe under Cd exposure. Cd supply strongly decreased the activities of antioxidant enzymes in AV, while in the Cd resistant Jena these activities either remained unaffected (SOD, APX) or were increased (CAT) by Cd supply. In conclusion, naturally selected Cd-tolerance in Thlaspi arvense is due to efficient Cd exclusion. The mechanisms underlying exclusion of Cd from the shoots seem Cd-specific yet they did not affect the homeostasis of Fe and Zn in the shoots.

Keywords: ascorbate peroxidase; catalase; iron; superoxide dismutase; zinc
Subjects: cadmium; ascorbate peroxidase; catalase; iron; superoxide dismutase; zinc; potassium; magnesium; calcium; manganese

Received: June 15, 2011; Accepted: October 26, 2011; Published: December 1, 2012  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Martin, S.R., Llugany, M., Barceló, J., & Poschenrieder, C. (2012). Cadmium exclusion a key factor in differential Cd-resistance in Thlaspi arvense ecotypes. Biologia plantarum, 56(4), 729-734. doi: 10.1007/s10535-012-0056-8
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Baker, A.J.M., Reeves, R.D., Hajar, A.S.M.: Heavy metal accumulation and tolerance in British populations of the metallophyte Thlaspi caerulescens J.& C. Presl (Brassicaceae). - New Phytol. 127: 61-68, 1994. Go to original source...
    2. Barceló, J., Poschenrieder, C., Cabot, C.: [Cadmium, manganese, iron, zinc and magnesium content of bean plants (Phaseolus vulgaris L.) in relation to the duration and the amount of cadmium supply]. - Z. Pflanzenernähr. Bdk. 148: 278-288, 1985. [In German]. Go to original source...
    3. Barceló, J., Vázquez, M.D., Poschenrieder, C.: Structural and ultrastructural disorders of cadmium-treated bush bean plants (Phaseolus vulgaris L.). - New Phytol. 108: 37-49, 1989. Go to original source...
    4. Bergmann, W. (ed.): Ernährungsstörungen bei Kullturpflanzen - Spektrum Akademischer Verlag, Heidelberg 1993.
    5. Bonet, A., Poschenrieder, C., Barceló, J.: Chromium III-iron interaction in Fe-deficient and Fe-sufficient bean plants. I. Growth and nutrient content. - J. Plant Nutr. 14: 403-414, 1991. Go to original source...
    6. Cakmak, I., Öztürk, L., Eker, S., Torun, B., Kalfa, HI., Yilmaz, A.: Concentration of zinc and activity of copper/zincsuperoxide dismutase in leaves of rye and wheat cultivars differing in sensitivity to zinc deficiency. - J. Plant Physiol. 151: 91-95, 1997. Go to original source...
    7. Cakmak, I.: Enrichment of fertilizers with zinc: an excellent investment for humanity and crop production in India. - J. Trace Element Med. Biol. 23: 281-289, 2009. Go to original source...
    8. Clemens, S.: Toxic metal accumulation, responses to exposure and mechanisms of tolerance in plants. - Biochimie 88: 1707-1719, 2006. Go to original source...
    9. Epstein, E.: Mineral Nutrition of Plants: Principles and Perspectives. - John Wiley and Sons, New York 1972.
    10. Grant, C.A., Clarke, J.M., Duguid, S., Chaney, R.L.: Selection and breeding of plant cultivars to minimize cadmium accumulation. - Sci. Total Environ. 390: 301-310, 2008. Go to original source...
    11. Hammond, J.P., Bowen, H.C., White, P.J. et al.: A comparison of the Thlaspi caerulescens and Thlaspi arvense transcriptomes. - New Phytol. 170: 239-260, 2006. Go to original source...
    12. Hart, J.J., Welch, R.M., Norvell, W.A., Kochian, LV.: Characterization of cadmium uptake, translocation and storage in near-isogenic lines of durum wheat that differ in grain cadmium concentration. - New Phytol. 172: 261-271, 2006. Go to original source...
    13. Kinraide, T.B.: Improved scales for metal ion softness and toxicity. Environ. - Toxicol. Chem. 28: 525-533, 2009. Go to original source...
    14. Lombi, E., Zhao, F.J., Dunham, S.J., McGrath, S.P.: Cadmium accumulation in populations of Thlaspi caerulescens and Thlaspi goesingense. - New Phytol. 145: 11-20, 2000. Go to original source...
    15. Lux, A., Martinka, M., Vaculík, M., White, P.J.: Root responses to cadmium in the rhizosphere: a review. - J. exp. Bot. 62: 21-37, 2011. Go to original source...
    16. Marschner, H.: Mineral Nutrition of Higher Plants. 2nd Ed. - Academic Press, London - San Diego - New York - Boston - Sydney - Tokyo - Toronto 1995.
    17. Milner, M.J., Kochian, L.V.: Investigating heavy-metal hyperaccumulation using Thlaspi caerulescens as a model system. - Ann. Bot. 102: 3-13, 2008. Go to original source...
    18. Ozturk L., Karanlik, S., Ozkutlu, F., Cakmak, I., Kochian, LV.: Shoot biomass and zinc/cadmium uptake for hyperaccumulator and non-accumulator Thlaspi species in response to growth on a zinc-deficient calcareous soil. - Plant Sci. 164: 1095-1101, 2003. Go to original source...
    19. Poschenrieder, C., Gunsé, B., Barceló, J.: Influence of cadmium on water relations, stomatal resistance, and abscisic acid content in expanding bean leaves. - Plant Physiol. 90: 1365-1371, 1989. Go to original source...
    20. Qiu, R-L, Zhao, X., Tang, Y-T., Yu, F-M., Hu, P-J.: Antioxidative response to Cd in a newly discovered cadmium hyperaccumulator, Arabis paniculata F. - Chemosphere 74: 6-12, 2008. Go to original source...
    21. Rodríguez-Serrano, M., Romero-Puertas, M.C., Pazmino, D.M., Testillano, PS., Risueno, MC., del Rio, LA., Sandalio, LM.: Cellular response of pea plants to cadmium toxicity: cross talk between reactive oxygen species, nitric oxide, and calcium. - Plant Physiol. 150: 229-243, 2009. Go to original source...
    22. Romero-Puertas, M.C., McCarthy, I., Sandalio, L.M., Palma, J.M., Gómez, M., Del Río, L.A.: Cadmium toxicity and oxidative metabolism of pea leaf peroxisomes. - Free Radical Res. 31(Suppl.): S25-S31, 1999. Go to original source...
    23. Sandalio, L.M., Dalurzo, H.C., Gómez, M., Romero-Puertas, MC., Del Rio, LA. Cadmium-induced changes in the growth and oxidative metabolism of pea plants. - J. exp. Bot. 52: 2115-2126, 2001. Go to original source...
    24. Sanità di Toppi L., Gabbrielli, R.: Response to cadmium in higher plants. - Environ. exp. Bot. 41: 105-130, 1999.
    25. Schat, H., Vooijs, R., Kuiper, E.: Identical major gene loci for heavy metal tolerances have independently evolved in different local populations and subspecies of Silene vulgaris. - Evolution 50: 1888-1895, 1996. Go to original source...
    26. Schat, H., Llugany, M., Bernhard, R.: Metal-specific patterns of tolerance, uptake, and transport of heavy metals in hyperaccumulating and non-hyperaccumulating metallophytes. - In: Terry, N., Bañuelos, G. (ed.): Phytoremediation of Contaminated Soil and Water. Pp 171-188. Lewis Publishers, Boca Raton 2000. Go to original source...
    27. Shanmugam, V., Lo, J.-C., Wu, C.-L., Wang, SL., Lai, CC., Connolly, EL., Huang, JL., Yeh, KC.: Differential expression and regulation of iron-regulated metal transporters in Arabidopsis halleri and Arabidopsis thaliana - the role in zinc tolerance. - New Phytol. 190: 125-137, 2011. Go to original source...
    28. Siedlecka, A., Krupa, Z.: Cd/Fe interactions in higher plants-its consequences for the photosynthetic apparatus. - Photosynthetica 36: 321-331, 1999. Go to original source...
    29. Singh, B.R., Gupta, S.K., Azaizeh, H., Shilev, S., Sudre, D., Song, WY., Martinoia, E., Mench, M.: Safety of food crops on land contaminated with trace elements. - J. Sci. Food Agr. 91: 1349-1366, 2009. Go to original source...
    30. Tolrà, R., Pongrac, P., Poschenrieder, C., Vogel-Mikus, K., Regvar, M., Barcelo, J.: Distinctive effects of cadmium on glucosinolate profiles in Cd hyperaccumulator Thlaspi praecox and non-hyperaccumulator Thlaspi arvense. - Plant Soil 288: 333-341, 2006. Go to original source...
    31. Verbruggen, N., Hermans, C., Schat, H.: Mechanisms to cope with arsenic or cadmium excess in plants. - Curr. Opin. Plant Biol. 12: 364-372, 2009. Go to original source...
    32. Verkleij, J.A.C., Prast, J.E.: Cadmium tolerance and cotolerance in Silene vulgaris (Moench.) Garcke [S. cucubalus (L.) Wib]. - New Phytol. 111: 637-645, 1989. Go to original source...
    33. Watanabe, A., Ito, H., Chiba, M., Ito, A., Shimizu, H., Fuji, S., Nakamura, S., Hattori, H., Chino, M., Satoh-Nagasawa, N., Takahashi, H., Sakurai, K., Akagi, H.: Isolation of novel types of Arabidopsis mutants with altered reactions to cadmium: cadmium-gradient agar plates are an effective screen for the heavy metal-related mutants. - Planta 232: 825-836, 2010. Go to original source...
    34. Wójcik, M., Tukiendorf, A..: Glutathione in adaptation of Arabidopsis thaliana to cadmium stress. - Biol. Plant. 55: 125-132, 2011. Go to original source...
    35. Zacchini, M., Iori, V., Scarascia Mugnozza, G., Pietrini, F., Massacci, A.: Cadmium accumulation and tolerance in Populus nigra and Salix alba. - Biol. Plant. 55: 383-386, 2011. Go to original source...
    36. Zelko, I., Lux, A., Czibulka, K.: Difference in the root structure of hyperaccumulator Thlaspi caerulescens and nonhyperaccumulator Thlaspi arvense. - Int. J. Environ Pollut 33: 123-132, 2008. Go to original source...

    Return to the content