biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 57:385-389, 2013 | DOI: 10.1007/s10535-012-0288-7

The effect of arsenic on pigment composition and photosynthesis in Hydrilla verticillata

S. Srivastava1,*, A. K. Srivastava1, B. Singh2, P. Suprasanna1, S. F. D'souza1
1 Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai, India
2 Nuclear Research Laboratory, Indian Agricultural Research Institute, New Delhi, India

The present study evaluated the effects of 100 and 500 μM arsenate (Na2HAsO4) on pigment composition and photosynthesis in Hydrilla verticillata (L.f.) Royle. Arsenic accumulation increased in concentration and duration dependent manner. The maximum accumulation [568 μg(As) g-1(d.m.)] was observed at 500 μM concentration and 96-h exposure. This concentration led to a significant decline in chlorophyll a content and PS II efficiency during the whole experiment, and in chlorophyll b and carotenoids after 96 h, but no significant changes in photosynthetic pigments were noticed at 100 μM arsenate. Net photosynthetic rate, electron transport rate, and water use efficiency declined whereas transpiration rate increased, and stomatal conductance and photochemical quenching did not show any effect or increased. The content of reactive oxygen species increased and content of reduced ascorbate declined at 500 μM arsenate in comparison to the control.

Keywords: ascorbate; chlorophyll; electron transport rate; photosynthetic efficiency; stomatal conductance; superoxide radical; transpiration rate; water use efficiency
Subjects: arsenic; ascorbic acid; chlorophyll; electron transport rate; photosynthetic rate; stomatal conductance; transpiration rate; water use efficiency; carotenoids; chlorophyll fluorescence; oxidative stress

Received: July 10, 2012; Accepted: August 24, 2012; Published: June 1, 2013  Show citation

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Srivastava, S., Srivastava, A.K., Singh, B., Suprasanna, P., & D'souza, S.F. (2013). The effect of arsenic on pigment composition and photosynthesis in Hydrilla verticillata. Biologia plantarum, 57(2), 385-389. doi: 10.1007/s10535-012-0288-7
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