biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 57:465-473, 2013 | DOI: 10.1007/s10535-013-0309-1

Isolation and functional characterization of Salt overly sensitive 1 (SOS1) gene promoter from Salicornia brachiata

E. Goyal1, R. S. Singh1, K. Kanika1,*
1 Biotechnology and Climate Change Laboratory, National Research Center on Plant Biotechnology, New Delhi, India

Soil salinity is a major abiotic stress and salt overly sensitive (SOS) pathway plays an important role in imparting tolerance to salinity by reinstating cellular ionic equilibrium. Salt overly sensitive 1 (SOS1) gene of SOS pathway has been implicated in increasing salt tolerance in plants. In this study, a 734 bp fragment of SOS1 promoter (SbUSOS1) was isolated from a halophyte Salicornia brachiata Roxb. In silico analysis of SbUSOS1 predicted several cis-acting regulatory elements such as DOF motif, GT elements, ABRE-like sequence, and root specific motifs. Functional validation of SbUSOS1 into tobacco stems and leaves using the GUS reporter gene showed that this promoter is induced by salt stress (250 mM NaCl) but not by ABA (500 μM) and cold (4 °C) stresses. This study indicated that SbUSOS1 was functional with predicted cis-acting elements that could be responsible for its salt-inducible nature. It can be used for the development of salt stress tolerant transgenic plants.

Keywords: abscisic acid; Agrobacterium tumefaciens; cold stress; β-glucuronidase; NaCl; salt-inducible promoter
Subjects: gene expression; gene promoter; abscisic acid; temperature - low; β-glucuronidase; salt-inducible promoter; salinity; transgenic plants

Received: October 5, 2012; Accepted: November 7, 2012; Published: September 1, 2013  Show citation

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Goyal, E., Singh, R.S., & Kanika, K. (2013). Isolation and functional characterization of Salt overly sensitive 1 (SOS1) gene promoter from Salicornia brachiata. Biologia plantarum, 57(3), 465-473. doi: 10.1007/s10535-013-0309-1
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