biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 64:43-49, 2020 | DOI: 10.32615/bp.2019.133

Changes of lipid metabolism of Arabidopsis thaliana in response to oligochitosan treatment

X.J. WANG1, X.L. SU2, J.M. LI1,*, P. ZHU3
Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation, Taizhou University,
1 Taizhou, Zhejiang 318000, P.R. China
2 College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China
3 Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ningbo, Zhejiang 315211, P.R. China

Lipids are vital cellular constituents in plant, and lipid peroxidation metabolites are critical defence substances in plants. In this study, mass spectrometry along with projections to latent structures discriminant analysis (PLS-DA) was used to detect lipid metabolism changes in Arabidopsis thaliana in response to oligochitosan (an effective resistance elicitor for the control of plant diseases). The PLS-DA showed that lipid metabolites of Arabidopsis thaliana were influenced by oligochitosan treatment. The total content of oxylipin containing monogalactosyldiacylglycerols, oxylipin-containing digalactosyldiacylglycerols, and oxylipin-containing phosphatidylglycerols increased firstly (after 1 h), and then decreased with the increase of oligochitosan treatment duration. In contrast, the total content of monogalactosyldiacylglycerols, phosphatidylcholines, phosphatidyl-ethanolamine, and phosphatidylglycerols decreased firstly, and then increased with the increase of oligochitosan treatment duration. The amounts of free fatty acids (C16:2, C16:3, C18:2, and C18:3) were lower after treatment with oligochitosan for 1, 3, and 6 h than in the control, while the production of volatile organic compounds such as 2-hexenal (except for 3 h) and nonanal was higher than in the control. In conclusion, lipid metabolites of Arabidopsis thaliana were influenced by oligochitosan treatment, and the synthesized lipids and oxylipin-containing lipids were remodelled and free fatty acids was metabolized to volatile organic compounds.

Keywords: free fatty acids, oxylipin-containing lipids, volatile organic compounds.

Received: February 19, 2019; Revised: September 30, 2019; Accepted: October 23, 2019; Published online: January 13, 2020  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
WANG, X.J., SU, X.L., LI, J.M., & ZHU, P. (2020). Changes of lipid metabolism of Arabidopsis thaliana in response to oligochitosan treatment. Biologia plantarum, 64, Article 43-49. https://doi.org/10.32615/bp.2019.133
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    Supplementary files

    Download fileWang6189 Suppl.pdf

    File size: 303.97 kB

    References

    1. Bejaoui, F., Salas, J.J., Nouairi, I., Smaoui, A., Abdelly, C., Martínez-Force, E., Youssef, N.B.: Changes in chloroplast lipid contents and chloroplast ultrastructure in Sulla carnosa and Sulla coronaria leaves under salt stress. - J. Plant Physiol. 198:32-38, 2016. Go to original source...
    2. Bligh, E.G., Dyer, W.J.: A rapid method of total lipid extraction and purification. - Can. J. Biochem. Physiol. 37: 91-917, 1959. Go to original source...
    3. Buseman, C.M., Tamura, P., Sparks, A.A., Baughman, E.J., Maatta, S., Zhao, J., Roth, M.R., Esch, S.W., Shah, J., Williams, T.D., Welti, R.: Wounding stimulates the accumulation of glycerolipids containing oxophytodienoic acid and dinor-oxophytodienoic acid in Arabidopsis leaves. - Plant Physiol. 142: 28-39, 2006. Go to original source...
    4. Chechetkin, I.R., Blufard, A.S., Khairutdinov, B.I., Mukhitova, F.K., Gorina, S.S., Yarin, A.Y., Antsygina, L.L., Grechkin, A.N.: Isolation and structure elucidation of linolipins C and D, complex oxylipins from flax leaves. - Phytochemistry 96:110-116, 2013. Go to original source...
    5. Chen, Y.F., Zhan, Y., Zhao, X.M., Guo, P., An, H.L,, Du, Y.G,, Han,Y.R., Liu, H., Zhang, Y.H.: Functions of oligochitosan induced protein kinase in tobacco mosaic virus resistance and pathogenesis related proteins in tobacco. - Plant Physiol. Biochem. 47: 724-731, 2009. Go to original source...
    6. Croisier, E., Rempt, M., Pohnert, G.: Survey of volatile oxylipins and their biosynthetic precursors in bryophytes. - Phytochemistry 71: 574-580, 2010. Go to original source...
    7. Elkahoui, S., Smaoui, A., Zarrouk, M., Ghrir, R., Limam, F.: Salt-induced lipid changes in Catharanthus roseus cultured cell suspensions. - Phytochemistry 65: 1911-1917, 2004. Go to original source...
    8. Feussner, I., Wasternack, C.: The lipoxygenase pathway. - Annu. Rev. Plant Biol. 53: 275-297, 2002. Go to original source...
    9. Genva, M., Akong, F.O., Andersson, M.X., Deleu, M., Lins, L., Fauconnier, M.L.: New insights into the biosynthesis of esterified oxylipins and their involvement in plant defense and developmental mechanisms. - Phytochem. Rev. ??: 1-16, 2018. Go to original source...
    10. Gigon, A., Matos, A.R., Laffray, D., Zuilyfodil, Y., Phamthi, A.T.: Effect of drought stress on lipid metabolism in the leaves of Arabidopsis thaliana (ecotype Columbia). - Ann. Bot. 94: 345-351, 2004. Go to original source...
    11. Gigot, C., Ongena, M., Fauconnier, M.L., Wathelet, J.P., Jardin, P. D., Thonart, P.: The lipoxygenase metabolic pathway in plants: potential for industrial production of natural green leaf volatiles. - Biotechnol. Agron. Soc. Environ. 14: 451-460, 2010.
    12. Kishimoto, K., Matsui, K., Ozawa, R., Takabayashi, J.: Components of C6-aldehyde-induced resistance in Arabidopsis thaliana against a necrotrophic fungal pathogen, Botrytis cinerea. - Plant Sci. 170: 715-723, 2006. Go to original source...
    13. Kupper, F.C., Gaquerel, E., Boneberg, E.M., Morath, S., Salaun, J.P., Potin, P.: Early events in the perception of lipopolysaccharides in the brown alga Laminaria digitata include an oxidative burst and activation of fatty acid oxidation cascades. -J. exp. Bot. 57: 1991-1999, 2006. Go to original source...
    14. Li, S., Xu, J.L., Chen J, Chen, J.J., Zhou, C.X., Yan, X.J.: The major lipid changes of some important diet microalgae during the entire growth phase. - Aquaculture 428-429: 104-110, 2014. Go to original source...
    15. Liaqata, F., Eltem, R.: Chitooligosaccharides and their biological activities: a comprehensive review. - Carbohydr. Polymers 184: 243-259, 2018. Go to original source...
    16. Matsui, K., Minami, A., Hornung, E., Shibata, H., Kishimoto, K., Ahnert, V., Kindl, H,, Kajiwara, T., Feussner, I.: Biosynthesis of fatty acid derived aldehydes is induced upon mechanical wounding and its products show fungicidal activities in cucumber. - Phytochemistry 67: 649-657, 2006. Go to original source...
    17. Mauch-Mani, B., Slusarenko, A.J.: Arabidopsis as a model host for studying plant-pathogen interactions. -Trends Microbiol. 1: 265-270, 1993. Go to original source...
    18. Meinke, D.W., Cherry, J.M., Dean, C., Rounsley S.D., Koornneef, M.: Arabidopsis thaliana: a model plant for genome analysis. - Science 282: 662-682, 1998. Go to original source...
    19. Meyerowitz, E.M., Bowman, J.L., Brockman, L.L., Drews, G.N., Jack, T., Sieburth, L.E., Weigel, D.: A genetic and molecular model for flower development in Arabidopsis thaliana. - Develompment 1 (Suppl.): 157-167, 1991. Go to original source...
    20. Pohnert, G.: Phospholipase A2 activity triggers the wound-activated chemical defense in the diatom Thalassiosira rotula. - Plant Physiol. 129: 103-111, 2002. Go to original source...
    21. Roberts, L.D., McCombie, G., Titman, C.M., Griffin, J.L.: A matter of fat: an introduction to lipidomic profiling methods. - J. Chromatogr. B. 871: 174-181, 2008. Go to original source...
    22. Scala, A., Allmann, S., Mirabella, R., Haring, M. A., Schuurink, R.C.: Green leaf volatiles: a plant's multifunctional weapon against herbivores and pathogens. - Int. J. mol. Sci. 14: 17781-17811, 2013. Go to original source...
    23. Stumpe, M., Bode, J., Göbel, C., Wichard, T., Schaaf, A., Frank, W., Frank, M., Reski, R., Pohnert, G., Feussner, I.: Biosynthesis of C9-aldehydes in the moss Physcomitrella patens. - Biochim. Bbophys. Acta. 1761: 301-312, 2006. Go to original source...
    24. Vaughn, S.F., Gardner. H.W.: Lipoxygenase-derived aldehydes inhibit fungi pathogenic on soybean. -J. chem. Ecol. 19: 2337-2345, 1993. Go to original source...
    25. Vu, H.S., Shiva, S., Roth, M.R., Tamura, P., Zheng, L., Li, M., Sarowar, S., Honey, S., McEllhiney, D., Hinkes, P., Seib, L., Williams, T.D., Gadbury, G., Wang, X., Shah, J., Welti, R.: Lipid changes after leaf wounding in Arabidopsis thaliana: expanded lipidomic data form the basis for lipid co-occurrence analysis. - Plant J. 80: 728-743, 2014. Go to original source...
    26. Wang, X.J., Li, J.M., Zhu, P.: Effect of Sclerotinia sclerotiorum on lipid metabolism in Arabidopsis thaliana. - J. Plant Dis. Protect. 124: 421-426, 2017. Go to original source...
    27. Wang, S.Y., Lin, H.S.: Effect of plant growth temperature on membrane lipids in strawberry (Fragaria × ananassa Duch.). - Sci. Hort. 108: 35-42, 2006. Go to original source...
    28. Wang, X.J., Su, X.L., Luo, Q.J., Xu, J.L., Chen, J.J., Yan, X.J., Chen, H.M.: Profiles of glycerolipids in Pyropia haitanensis and their changes responding to agaro-oligosaccharides. -J. appl. Phycol. 26: 2397-2404, 2014. Go to original source...
    29. Wang, X.M.: Lipid signaling. - Curr. Opin. Plant Biol. 7: 329-336, 2004. Go to original source...
    30. Wang, X.M., Li, W.Q., Li, M.Y., Welti, R.: Profiling lipid changes in plant response to low temperatures. - Physiol. Plant. 126: 90-96, 2006. Go to original source...
    31. Welti, R., Li, W., Li, M., Sang, Y., Biesiada, H., Zhou, H.E., Rajashekar, C.B., Williams, T.D., Wang, X.: Profiling membrane lipids in plant stress responses. Role of phospholipase D alpha in freezing-induced lipid changes in Arabidopsis. - J. biol. Chem. 277: 31994-32002, 2002. Go to original source...
    32. Yin, H., Li, S., Zhao, X., Du, Y., Ma, X.: cDNA microarray analysis of gene expression in Brassica napus treated with oligochitosan elicitor. - Plant Physiol. Biochem. 44: 910-916, 2006. Go to original source...
    33. Yin, H., Zhao, X., Du, Y.: Oligochitosan: a plant diseases vaccine. A review. - Carbohydr. Polymers 82: 1-8, 2010. Go to original source...
    34. Zhao, X.M., She, X.P., Du, Y.G., Liang, X.M.: Induction of antiviral resistance and stimulary effect by oligochitosan in tobacco. - Pestic. Biochem. Physiol. 87: 78-84, 2007a. Go to original source...
    35. Zhao, X.M., She, X.P., Liang, X.M., Du, Y.G.: Effects of oligochitosans on tobacco cells and role of endogenous nitric oxide burst in the resistance of tobacco to Tobacco mosaic virus. - J. Plant Pathol. 89: 69-79, 2007b.

    Return to the content