biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 65:203-211, 2021 | DOI: 10.32615/bp.2021.023

Physiological and biochemical changes during graft union formation in Carya illinoinensis

W.C. SU, H.Y. HE, Z.Z. LIU, Z.H. MO, F. CAO, F.R. PENG*
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, P.R. China

To reveal the physiological and biochemical mechanism underlying graft union formation in pecan (Carya illinoinensis), dynamic changes in content of nutrients, tannin, and phytohormones together with key enzyme activities were investigated 0, 3, 5, 7, 10, 14, 18, 22, 31, and 40 d after grafting (DAG), in homograft unions. During graft union formation, peroxidase (POD) activity increased at 7 - 10 DAG compared with that at 0 DAG. Polyphenol oxidase (PPO) activity was higher in grafted than ungrafted pecan seedlings (control) at 22 DAG, which was similar to POD activity. The tannin content exhibited a decreasing trend with grafting relative to the control. Indole-3-acetic acid (IAA) and zeatin riboside (ZR) content increased from 7 - 10 DAG, with higher average content than in the control at 14 - 31 DAG. Abscisic acid (ABA), soluble sugar, starch, and soluble protein content was generally lower in grafted plants than in the control. Combined with our previous anatomical observations, these results suggested that, during graft development, some enzymes and growth promoting hormones might be required for callus proliferation at early stage and for vascular reconnection at the later stage. Nutrients provided energy for the whole graft development process. In contrast, some polyphenols and growth inhibiting hormones seemed to have negative effects on this process.

Keywords: ABA, grafting, IAA, nutrients, pecan, peroxidase, polyphenol oxidase, tannin, zeatin riboside.

Received: December 21, 2020; Revised: March 22, 2021; Accepted: April 8, 2021; Published online: July 28, 2021  Show citation

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SU, W.C., HE, H.Y., LIU, Z.Z., MO, Z.H., CAO, F., & PENG, F.R. (2021). Physiological and biochemical changes during graft union formation in Carya illinoinensis. Biologia plantarum, 65, Article 203-211. https://doi.org/10.32615/bp.2021.023
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