biologia plantarum

Biologia plantarum 65:307-315, 2021 | DOI: 10.32615/bp.2021.039

Melatonin alleviates photoinhibition in cucumber seedlings by modulating partitioning of absorbed excitation energy in photosystem Ⅱ

H.L. ZHAO^{1, 2, 3}, Y.P. WANG^{1, *}, K. GAO^{1, 2}, Y. ZHANG^{1, 2, 3}, Y. SHI^{1, 2, 3}, Y.X. MIAO^{1, 2, 3}

¹ College of Horticulture, Shanxi Agricultural University, Taigu, 030801, Shanxi, P.R. China

² Shanxi protected vegetable quality improvement and Synergetic Innovation Center, Taigu, 030801, Shanxi, P.R. China

³ Shanxi Key Laboratory of Facility Horticulture, Taigu, 030801,Shanxi, P.R. China

The aim of this study was to evaluate the effects of melatonin on photoinhibition under chilling stress in cucumber seedlings and to inquire into any mechanisms of mitigation. Under chilling stress, the net photosynthetic rate declined dramatically but the decline was significantly mitigated by irrigation with a melatonin solution. Possible mechanisms for this mitigation are that melatonin accelerates xanthophyll de-epoxidation by upregulating the transcription of the violaxanthin de-epoxidase gene (CsVDE) and down-regulating that of the zeaxanthin cyclase gene (CsZE) during chilling. There was also a rise in non-photochemical quenching (NPQ) if seedlings were pretreated with melatonin before chilling. The efficient operation of the xanthophyll cycle helped consume excessive excitation energy in photosystem (PS) Ⅱ and so protected the photosynthetic system. Melatonin also modulated the partitioning of absorbed excitation energy in PS Ⅱ as evidenced by alleviation of the decrease in quantum yield of photochemical energy conversion in PS Ⅱ under chilling stress, by alleviation of the rise in quantum yield of non-regulated, non-photochemical energy loss in PS II and by increasing the regulated non-photochemical energy loss in PS II. This study presents a new understanding of the mechanisms through which melatonin mitigates photoinhibition by modulating the partitioning of absorption energy in PS Ⅱ based on the xanthophyll cycle.

Keywords: chilling, Cucumis sativus, excitation energy, melatonin, photoinhibition, xanthophyll cycle.

Received: October 11, 2020; Revised: June 7, 2021; Accepted: June 30, 2021; Published online: November 12, 2021  Show citation