biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 66:96-102, 2022 | DOI: 10.32615/bp.2021.065

Regulation of StTCP15 gene expression and tuber dormancy characteristics of potato by gibberellic acid, abscisic acid, and low temperature

Y. CHE1, 2, 3, Y.-Q. LIAO1, X. FU1, 2, Y. YUE4, N. ZHANG1, 2, *, H.-J. SI1, 2
1 College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, P.R. China
2 Gansu Provincial Key Laboratory of Arid Land Crop Science, Gansu Agricultural University, Lanzhou 730070, P.R. China
3 Key Laboratory of Functional Genomic and Molecular Diagnosis of Gansu Province, Lanzhou 730070, P.R. China
4 Gansu Pharmaceutical Investment Group Co., Lanzhou 730030, P.R. China

Potato (Solanum tuberosum L.) cv. Eshu 10 was used to investigate the effects of exogenous gibberellic acid (GA3), abscisic acid (ABA), and low-temperature stress on changes of hormone content, expression patterns of StTCP15 gene, and tuber dormancy characteristics. Under GA3 treatment and low-temperature stress, tuber dormancy was broken in about one week sooner compared with the control group, but ABA treatment did not significantly promote the breaking of tuber dormancy. The results of hormone determination using liquid chromatography-mass spectrometry (LC-MS/MS) showed that the content of ABA in tubers treated with GA3 or low-temperature stress was lower than in the control group, and it was higher than in the control group under ABA treatment. The GA3 content of tubers was higher than in the control group under GA3 treatment and lower under low-temperature stress. During dormancy, the ABA content continued to increase and GA3 content fluctuated, ABA content rapidly decreased and GA3 content rapidly increased when the dormancy was breaking, and both ABA content and GA3 content increased during germination. The results from the assay of real-time quantitative PCR showed that the expression of the StTCP15 gene was continuously increased during the dormant period in all groups, and the expression of the StTCP15 gene was the highest at the time of dormancy release. The expression of the StTCP15 gene was increased about 15 times on the 7th d under low-temperature stress and was restored at room temperature. Thus, the StTCP15 gene can respond to GA3, ABA, and low-temperature stress and may be involved in the release of potato tuber dormancy.

Keywords: abscisic acid, gibberellin, low temperature, potato, Solanum tuberosum, StTCP15 gene, tuber dormancy.

Received: March 17, 2021; Revised: August 25, 2021; Accepted: November 3, 2021; Published online: April 7, 2022  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
CHE, Y., LIAO, Y.-Q., FU, X., YUE, Y., ZHANG, N., & SI, H.-J. (2022). Regulation of StTCP15 gene expression and tuber dormancy characteristics of potato by gibberellic acid, abscisic acid, and low temperature. Biologia plantarum, 66, Article 96-102. https://doi.org/10.32615/bp.2021.065
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    Supplementary files

    Download file6709_Che_Suppl.pdf

    File size: 167.91 kB

    References

    1. Alamar, M.C., Tosetti, R., Landahl, S., Bermejo, A., Terry, L.A.: Assuring potato tuber quality during storage: a future perspective. - Front. Plant Sci. 8: 2034, 2017. Go to original source...
    2. Brady, S.M., McCourt, P.: Hormone cross-talk in seed dormancy. - J. Plant Growth Reg. 22: 25-31, 2003. Go to original source...
    3. Chen, L., Chen, Y.Q., Ding, A.M., Chen, H., Xia, F., Wang, W.F., Sun, Y.H.: Genome-wide analysis of TCP family in tobacco. - Genet. mol. Res. 15: (2), 2016. Go to original source...
    4. Coleman, W.K., King, R.R.: Changes in endogenous abscisic acid, soluble sugars and proline levels during tuber dormancy in Solanum tuberosum L. - Amer. Potato J. 61: 437-449, 1984. Go to original source...
    5. Davière, J., Wild, M., Regnault, T., Baumberger, N., Eisler, H., Genschik, P., Patrick, A.: Class I TCP-DELLA interactions in inflorescence shoot apex determine plant height. - Curr. Biol. 24: 1923-1928, 2014. Go to original source...
    6. Deng, C.L.: Potato dormancy and its breaking methods. - Chin. Potato J. 24: 151-152, 2010.
    7. Ding, J., Shen, Z.D., Fang, Y.X., Feng, X.X., Li, L., Ning, J.S.: Extraction, isolation and biological identification of plant endogenous hormones. - Plant Physiol. Commun. 2: 27-39+50, 1979.
    8. Ding, N., Li, Z.Z., Tian, W., Dang, R.M., Liu, Y.Y., Wen, S.S.: Genome-wide identification of the wheat TCP gene family and its response to heat stress. - J. Triticeae Crops 38: 25-33, 2018.
    9. Doebley, J., Stec, A., Hubbard, L.: The evolution of apical dominance in maize. - Nature 386: 485-488, 1997. Go to original source...
    10. Feng, Z.J., Xu, S.C., Liu, N., Zhang, G.W., Hu, Q.Z., Gong, Y.M.: Molecular mechanisms and applications of TCP transcription factors in plants. - J. Plant Genet. Resour. 19: 112-121, 2018.
    11. Footitt, S., Douterelo-Soler, I., Clay, H., Finch-Savage, W.E.: Dormancy cycling in Arabidopsis seeds is controlled by seasonally distinct hormone-signaling pathways. - Proc. nat. Acad. Sci. USA 108: 20236-20241, 2011. Go to original source...
    12. Guo, Z., Fujioka, S., Blancaflor, E., Miao, S., Gou, X., Li, J.: TCP1 modulates brassinosteroid biosynthesis by regulating the expression of the key biosynthetic gene DWARF4 in Arabidopsis thaliana. - Plant Cell 22: 1161-1173, 2010. Go to original source...
    13. Kang, D.L.: Physiological and Biochemical Changes of Potato Atlantic Tuber During Dormancy, Sprouting and Cold Storage. - Master Degree Dissertation, Hunan Agricultural University, Changsha, Hunan Province, 2007.
    14. Kosugi, S., Ohashi, Y.: PCF1 and PCF2 specifically bind to cis elements in the rice proliferating cell nuclear antigen gene. - Plant Cell 9: 1607-1619, 1997. Go to original source...
    15. Lei, N., Yu, X., Li, S., Zeng, C., Zou, L., Liao, W., Peng, M.: Phylogeny and expression pattern analysis of TCP transcription factors in cassava seedlings exposed to cold and/or drought stress. - Sci. Rep. 7: 10016, 2017. Go to original source...
    16. Li, S., Zachgo, S.: TCP3 interacts with R2R3-MYB proteins, promotes flavonoid biosynthesis and negatively regulates the auxin response in Arabidopsis thaliana. - Plant J. 76: 901-913, 2013. Go to original source...
    17. Li, W.R., Zhang, S.Y., Tang, H., He, L.X.: Effect of exogenous gibberellic acid on Paeonia rockii seeds germination. - Acta bot. boreal.-occident. sin. 39: 1819-1826, 2019.
    18. Liu, Y.S., Li, C., Wang, D.X., Xu, G., Wang, Y.P., Cheng, L.X., Zhang, J.L., Wang, D., Zhang, F.: Correlation among endogenous hormone content, threshold and dormancy periods in processing potato varieties minitubers during storage. - Sci. agr. sin. 48: 262-269, 2015.
    19. Livak, K., Schmittgen, T.: Analysis of relative gene expression data using real-time quantitative PCR and the 2-∆∆CT method. - Methods 25: 402-408, 2001. Go to original source...
    20. Lucero, L.E., Uberti-Manassero, N.G., Arce, A., Colombatti, F., Alemano, S.G., Gonzalez, D.H.: TCP15 modulates cytokinin and auxin responses during gynoecium development in Arabidopsis. - Plant J. 84: 267-282, 2015. Go to original source...
    21. Luo, D., Carpenter, R., Vincent, C., Copsey, L., Coen, E.: Origin of floral asymmetry in antirrhinum. - Nature 383: 794-799, 1996. Go to original source...
    22. Manassero, N.G.U., Viola, I.L., Welchen, E., Gonzalez, D.H.: TCP transcription factors: architectures of plant form. - BioMolecular Concepts 4: 111-127, 2013. Go to original source...
    23. Martín-Trillo, M., Cubas, P.: TCP genes: a family snapshot ten years later. - Trends Plant Sci. 15: 31-39, 2010. Go to original source...
    24. Navaud, O., Dabos, P., Carnus, E., Tremousaygue, D., Christine, H.: TCP transcription factors predate the emergence of land plants. - J. mol. Evol. 65: 23-33, 2007. Go to original source...
    25. Resentini, F., Felipo-Benavent, A., Colombo, L., Blázquez, M.A., Alabadi, D., Masiero, S.: TCP14 and TCP15 mediate the promotion of seed germination by gibberellins in Arabidopsis thaliana. - Mol. Plant. 8: 482-485, 2015. Go to original source...
    26. Riechmann, J.L., Heard, J., Martin, G., Reuber, L., Jiang, C., Keddie, J., Adam, L., Pineda, O., Ratcliffe, O.J., Samaha, R.R., Creelman, R., Pilgrim, M., Broun, P., Zhang, J.Z., Ghandehari, D., Sherman, B.K., Yu, G.: Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. - Science 290: 2105-2110, 2000. Go to original source...
    27. Rueda-Romero, P., Barrero-Sicilia, C., Gómez-Cadenas, A., Carbonero, P., Oñate-Sánchez, L.: Arabidopsis thaliana DOF6 negatively affects germination in non-after-ripened seeds and interacts with TCP14. - J. exp. Bot. 63: 1937-1949, 2011. Go to original source...
    28. Sonnewald, S., Sonnewald, U.: Regulation of potato tuber sprouting. - Planta 239: 27-38, 2014. Go to original source...
    29. Suzuki, T., Sakurai, K., Ueguchi, C., Mizuno, T.: Two types of putative nuclear factors that physically interact with histidine-containing phosphotransfer (Hpt) domains, signaling mediators in His-to-Asp phosphorelay, in Arabidopsis thaliana. - Plant Cell Physiol. 42: 37-45, 2001. Go to original source...
    30. Tatematsu, K., Nakabayashi, K., Kamiya, Y., Nambara, E.: Transcription factor AtTCP14 regulates embryonic growth potential during seed germination in Arabidopsis thaliana. - Plant J. 53: 42-52, 2008. Go to original source...
    31. Tang, X., Zhang, N., Si, H.J., Calderón-Urrea, A.: Selection and validation of reference genes for RT-qPCR analysis in potato under abiotic stress. - Plant Methods 13: 85, 2017. Go to original source...
    32. Wang, Y., Zhang, N., Li, T., Yang, J.W., Zhu, X., Fang, C.X., Li, S.G., Si, H.J.: Genome-wide identification and expression analysis of StTCP transcription factors of potato (Solanum tuberosum L.). - Comput. Biol. Chem. 78: 53-63, 2018. Go to original source...
    33. Xia, J., Shi, X.J., Hao, X.Z., Li, N.N., Tian, Y., Li, J.H., Luo, H.H.: Effect of low temperature on enzyme activity and hormone content of different genotype cotton species during germination. - Plant Physiol. J. 55: 1291-1305, 2019.
    34. Xiong, Y., Liu, T., Tian, C., Sun, S., Li, J., Chen, M.: Transcription factors in rice: a genome-wide comparative analysis between monocots and eudicots. - Plant mol. Biol. 59: 191-203, 2005. Go to original source...
    35. Yang, W.L., Sui, Q.J.: Study of the dormancy characteristic and controlling of different gene type mini-tuber stuffs. - J. Mountain Agr. Biol. 22: 5-8+12, 2003.
    36. Yao, X., Ma, H., Wang, J.J., Zhang, D.B.: Genome-wide comparative analysis and expression pattern of TCP gene families in Arabidopsis thaliana and Oryza sativa. - J. integr. Plant Biol. 49: 885-897, 2007. Go to original source...
    37. Ye, Y.X., Shen, Q.J., Xu, C.H.: Study on breaking the dormancy of basic seed of virus-eliminating potato by gibberellin. - Plant Physiol. Commun. 36: 123-125, 2000.
    38. Zhang, L.L., Chen, Y.L., Lian, Y.: Advances in the regulation of dormancy and dormancy in potato tubers. - Chin. Potato J. 06: 352-356, 2003.
    39. Zhong, L., Deng, J.C., Wang, L.J., Zheng, S.L., Dou, P., Wang, X.H., Yuan, J.C.: Effect of plant growth regulators on germination and oxidase activity during storage of potato tubers. - Acta pratacult. sin. 26: 147-157, 2017.
    40. Zhou, M., Li, D., Li, Z., Hu, Q., Yang, C., Zhu, L., Luo, H.: Constitutive expression of a mir319 gene alters plant development and enhances salt and drought tolerance in transgenic creeping bentgrass. - Plant Physiol. 161: 1375-1391, 2014. Go to original source...

    Return to the content