biologia plantarum

Biologia plantarum 66:188-200, 2022 | DOI: 10.32615/bp.2022.016

LC-MS/MS shotgun proteomics reveals biochemical mechanisms of Paspalum fasciculatum tolerance to Pb-stress

M. SALAS-MORENO^{1, 2}, M.Á. CASTILLEJO⁴, C. LÓPEZ-HIDALGO⁴, J. MARRUGO-NEGRETE³, E. RODRÍGUEZ-CAVALLO², D. MENDEZ-CUADRO^{2, *}, J. JORRÍN-NOVO^{4, *}

¹ Faculty of Basic Sciences, Biology Department, Biosystematic Research Group, Technological University of Chocó, Quibdó, 270002, Colombia

² Department of Biology, Analytical Chemistry and Biomedicine Group, University of Cartagena, Cartagena, 130015, Colombia

³ Chemistry Department, Water, Applied and Environmental Chemistry Group, University of Córdoba, Faculty of Basic Sciences, Montería, 230002, Colombia

⁴ Agroforestry and Plant Biochemistry, Proteomics, and Systems Biology Research Group, Department of Biochemistry and Molecular Biology-ETSIAM, University of Cordoba, UCO-CeiA3, 14071, Spain

Paspalum fasciculatum Willd. ex Flüggé grows in mining soils which are Cd- and Pb-contaminated where it exhibits tolerance to Pb and the ability to extract Pb from these soils. To elucidate tolerance mechanisms to Pb-stress, liquid chromatography with tandem mass spectrometry (LC-MS/MS) was used to quantify changes in the accumulation of proteins in leaves. We identified 323 proteins involved in primary metabolism and response to biotic or abiotic stresses. Although proteins involved in the processes of photosynthesis and saccharide and energy metabolism presented the greatest amount of down-regulated proteins, the plant was able to maintain photosynthetic functions and obtain energy to sustain the vital balance. P. fasciculatum based their tolerance on increased antioxidant defenses, improving the protection and repair of proteins and transduction signals to coordinate physiological response to Pb-stress. Our results provide important information to understand the tolerance mechanisms in P. fasciculatum and could be important in future molecular studies on the resistance and accumulation of Pb in plants.

Keywords: Paspalum fasciculatum, Pb-stress, shotgun proteomics, protein accumulation, tolerance mechanism.

Received: August 25, 2021; Revised: February 8, 2022; Accepted: April 1, 2022; Published online: August 8, 2022  Show citation