biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 66:201-206, 2022 | DOI: 10.32615/bp.2022.021

Okadaic acid did not change the nitrate reductase activation state in tomato seedlings

A. KOŁTON1, V. VA©TAKAITĖ-KAIRIENĖ2, *
1 Department of Botany, Physiology and Plant Protection, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, 31-120, Krakow, Poland
2 Lithuanian Research Centre for Agriculture and Forestry, Institute of Horticulture, LT-54333, Babtai, Lithuania

In this study, the total and actual nitrate reductase (NR) activity, and NR activation state, in tomato seedlings (Solanum lycopersicum cvs. Kmicic and Faworyt) treated with okadaic acid (OA) was evaluated. Seedlings were grown in a half-strength Murashige and Skoog (MS) medium in a growth chamber at day/night temperatures of 22/20 °C, a photon flux density of 150 µmol m-2 s-1, and a 16-h photoperiod. After 10 days, plants were transferred into MS medium with 0 (control), 0.01, 0.05, 0.1, 0.5, 1.0 µM OA. It was found that the total and actual NR activity increased in Kmicic leaves treated with 0.1, 0.5, and 1.0 µM OA compared to control. However, the NR activation state did not change in both roots and leaves of OA-treated tomato seedlings.

Keywords: nitrate, okadaic acid, protein phosphorylation, protein phosphatase inhibitor, Solanum lycopersicum.

Received: June 14, 2021; Revised: March 27, 2022; Accepted: April 21, 2022; Published online: August 26, 2022  Show citation

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KOŁTON, A., & VA©TAKAITĖ-KAIRIENĖ, V. (2022). Okadaic acid did not change the nitrate reductase activation state in tomato seedlings. Biologia plantarum, 66, Article 201-206. https://doi.org/10.32615/bp.2022.021
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