biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 67:184-188, 2023 | DOI: 10.32615/bp.2023.019

The accumulation of chlorophyll and changes of ATP during greening of etiolated Arabidopsis seedlings are mediated by nitric oxide

X.W. Da, L. Li, X. Wang, H.L. Pang, L.Y. Jia, K.Sun, J.Zhang, H.Q. Feng*
School of Life Science, Northwest Normal University, Lanzhou 730070, P.R. China

Seedling greening upon irradiance is essential for the survival of plant after germination. Here, we studied the role of nitric oxide (NO) in regulating the accumulation of chlorophyll during greening of Arabidopsis seedlings, and we also investigated the changes of ATP in the chloroplast and cytoplasm during greening by using a fluorescent protein sensor (Ateam 1.03-nD/nA) based on fluorescence resonance energy transfer (FRET) and the effects of NO on the changes of ATP content. The results showed that the content of NO, chlorophyll, and ATP in the chloroplast and cytoplasm increased with the increase of greening time. L-NAME, an inhibitor of NO, not only decreased the accumulation of chlorophyll content but also reduced the ATP content in the chloroplast and cytoplasm during the greening of Arabidopsis seedlings. Therefore, these experiments indicate that the accumulation of chlorophyll and changes of ATP during greening of etiolated Arabidopsis seedlings are mediated by nitric oxide.

Keywords: Arabidopsis thaliana, ATeam 1.03-nD/nA, ATP, chlorophyll, etiolation, greening, nitric oxide.

Received: August 4, 2022; Revised: May 10, 2023; Accepted: May 11, 2023; Published online: July 27, 2023  Show citation

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Da, X.W., Li, L., Wang, X., Pang, H.L., Jia, L.Y., Sun, K., Zhang, J., & Feng, H.Q. (2023). The accumulation of chlorophyll and changes of ATP during greening of etiolated Arabidopsis seedlings are mediated by nitric oxide. Biologia plantarum, 67, Article 184-188. https://doi.org/10.32615/bp.2023.019
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