biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 68:128-137, 2024 | DOI: 10.32615/bp.2024.005

Insights into some key parameters involved in the variability of tolerance to phosphorus deficiency in the legume model Medicago truncatula

W. M'SEHLI1, *, H. HOUMANI2, N. KALLALA1, 3, G. ABID1, I. HAMMAMI4, H. MHADHBI1
1 Laboratory of Legumes and Sustainable Agrosystems, Centre of Biotechnology of Borj-Cedria, 2050 Hammam-Lif, Tunisia
2 Laboratory of Extremophile Plants, Centre of Biotechnology of Borj-Cedria, 2050 Hammam-Lif, Tunisia
3 University Tunis El Manar, Faculty of Sciences of Tunis, 2092 Tunis, Tunisia
4 Biodiversity, Environment and Biotechnology-ISSBAT, Rue Zouheir Essaf, 1006 Tunis, Tunisia

Phosphorus is a key limiting factor for plant growth. Several approaches are developed to mitigate the impact of P shortage on plants and to the selection of crops with high P mobilizing capacity from P-deficient soils. In this work, four Medicago truncatula genotypes (A17, TN8.20, TN1.11, and TN6.18) were compared for their efficiency to cope with P limiting conditions using several criteria. Significant differences between genotypes, P deficiency treatments, and the interaction of genotypes with P deficiency treatments were found. P limitation resulted in an important decrease in shoot biomass, P content, P use efficiency, and photosynthetic parameters. A significant variability was found between the four genotypes, with A17 and TN8.20 being the most tolerant genotypes to P deficiency. This was consistent with the better ability of these genotypes to acidify rhizosphere and stimulate the activity of acid phosphatase and its relative gene (MtPAP1). The expression of P transporter genes (MtPT1, MtPT3, and MtPT5) was induced by P deficiency, however, the overexpression of those genes was more pronounced in tolerant genotypes. Overall, our data indicate that A17 and TN8.20 are more efficient in mobilizing P under limiting conditions and could be cultivated in P-deficient soils as forage crops.

Keywords: legumes, Medicago truncatula genotypes, P deficiency, P uptake.

Received: June 7, 2023; Revised: March 30, 2024; Accepted: April 3, 2024; Published online: August 16, 2024  Show citation

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M'SEHLI, W., HOUMANI, H., KALLALA, N., ABID, G., HAMMAMI, I., & MHADHBI, H. (2024). Insights into some key parameters involved in the variability of tolerance to phosphorus deficiency in the legume model Medicago truncatula. Biologia plantarum, 68, Article 128-137. https://doi.org/10.32615/bp.2024.005
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