biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 69:88-97, 2025 | DOI: 10.32615/bp.2025.008

Clonal variation of the basic resistance factors (POD, SOD, CAT, MDA, PRO) in Michelia chapensis

Houyin DENG, Jiamin HE, Rong HUANG, Ruping WEI, Runhui WANG, Su YAN, Guandi WU, Huiquan ZHENG*
Guangdong Provincial Key Laboratory of Silviculture, Protection and Utilization, Guangdong Academy of Forestry, Guangzhou 510520, P.R. China

Michelia chapensis Dandy, a rare and endangered evergreen woody species of the genus Michelia (Magnoliaceae), is endemic to China, with only scattered natural communities (Zhou et al., 2023). First reported from Lechang City, Guangdong Province in 1929 (Dandy, 1929), it is mainly distributed across southern China, with smaller populations in Vietnam (Sima et al., 2020). Valued for its fragrant flowers, medicinal properties, timber, and strong adaptability to diverse soil and climatic conditions (Ao, 1986; Chen et al., 2005; Wang et al., 2009; Liu et al., 2018; Zhou et al., 2023), M. chapensis also plays an important role in providing ecosystem services in both natural and urban environments (Cao et al., 2011; Chen, 2020).

Keywords: antioxidant enzymes, breeding, clonal variation, Michelia chapensis, non-enzymatic components.

Received: August 30, 2025; Revised: October 8, 2025; Accepted: November 10, 2025; Published online: December 2, 2025  Show citation

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DENG, H., Jiamin, H., HUANG, R., WEI, R., WANG, R., YAN, S., Guandi, W., & ZHENG, H. (2025). Clonal variation of the basic resistance factors (POD, SOD, CAT, MDA, PRO) in Michelia chapensis. Biologia plantarum, 69, Article 88-97. https://doi.org/10.32615/bp.2025.008
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    References

    1. Aebi, H. (1974) Catalase. In: Bergmeyer H.U. (Ed.) Methods of Enzymatic Analysis. Cambridge: Academic Press, pp. 673-684. Go to original source...
    2. Aebi, H. (1984) Catalase in vitro. Methods in Enzymology, 105, 121-126. Go to original source...
    3. Ao, H.X. (1986) [Superior landscaping fragrant-flowered tree: Michelia chapensis Dandy.] Guangdong Landscape Architecture, 1, 30. [In Chinese]
    4. Ashraf, M.F. & Foolad, M.R. (2007) Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany, 59, 206-216. Go to original source...
    5. Bates, L.S., Waldren, R.P. & Teare, I.D. (1973) Rapid determination of free proline for water-stress studies. Plant and Soil, 39, 205-207. Go to original source...
    6. Cao, X.-Y., Li, C.-G., Miao, Q., Zheng, Z.-J. & Jiang, J.-H. (2011) Molecular cloning and expression analysis of a leaf-specific expressing 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase gene from Michelia chapensis Dandy. Journal of Medicinal Plants Research, 5, 3868-3875.
    7. Chance, B. & Maehly, A.C. (1955) Assay of catalases and peroxidases. Methods in Enzymology, 2, 764-775. Go to original source...
    8. Chen, L. (2020) Study on carbon fixation and oxygen release ability of urban greening tree species based on spatial and temporal dynamic analysis. International Journal of Global Energy Issues, 42, 244-258. Go to original source...
    9. Chen, S., Qin, F., Yang, Y. et al. (2024) Extraction, purification, structural characterization, and bioactivities of the genus Schisandra polysaccharides: A review. International Journal of Biological Macromolecules, 262, 130257. Go to original source...
    10. Chen, Z., Liu, X., Wu, Z., Liu, P., Cheng, J. & Xia, N. (2005) Research on Magnoliaceae species geographic distribution and protect measures by using GIS of Guangdong province China. In: Proceedings. 2005 IEEE International Geoscience and Remote Sensing Symposium. IGARSS'05. Seoul: IEEE, pp. 4.
    11. Cosio, C. & Dunand, C. (2009) Specific functions of individual class III peroxidase genes. Journal of Experimental Botany, 60, 391-408. Go to original source...
    12. Dandy, J.E. (1929) Michelia chapensis. Journal of Botany: British and Foreign, 67, 223.
    13. Djerrad, Z., Kadik, L. & Djouahri, A. (2015) Chemical variability and antioxidant activities among Pinus halepensis Mill. essential oils provenances, depending on geographic variation and environmental conditions. Industrial Crops and Products, 74, 440-449. Go to original source...
    14. Engelhardt, K.A.M., Lloyd, M.W. & Neel, M.C. (2014) Effects of genetic diversity on conservation and restoration potential at individual, population, and regional scales. Biological Conservation, 179, 6-16. Go to original source...
    15. Fu, G. & Shen, Z.-X. (2017) Effects of enhanced UV-B radiation on plant physiology and growth on the Tibetan Plateau: a meta-analysis. Acta Physiologiae Plantarum, 39, 85. Go to original source...
    16. Fujita, M. & Hasanuzzaman, M. (2022) Approaches to enhancing antioxidant defense in plants. Antioxidants, 11, 925. Go to original source...
    17. Giannopolitis, C.N. & Ries, S.K. (1977) Superoxide dismutases: I. Occurrence in higher plants. Plant Physiology, 59, 309-314. Go to original source...
    18. Hasanuzzaman, M., Bhuyan, M.H.M.B., Zulfiqar, F. et al. (2020) Reactive oxygen species and antioxidant defense in plants under abiotic stress: revisiting the crucial role of a universal defense regulator. Antioxidants, 9, 681. Go to original source...
    19. Hasanuzzaman, M., Hossain, M.A., da Silva, J.A.T. & Fujita, M. (2011) Plant response and tolerance to abiotic oxidative stress: antioxidant defense is a key factor. In: Venkateswarlu, B., Shanker, A., Shanker, C. & Maheswari, M. (Eds.) Crop Stress and its Management: Perspectives and Strategies. Dordrecht: Springer, pp. 261-315. Go to original source...
    20. Hayat, S., Hayat, Q., Alyemeni, M.N., Wani, A.S., Pichtel, J. & Ahmad, A. (2012) Role of proline under changing environments: a review. Plant Signaling & Behavior, 7, 1456-1466. Go to original source...
    21. He, J.M. (2025) [Variation and transcriptomic studies on leaf traits and resistance-related enzyme activities of Michelia chapensis.] Master Thesis. Kunming: Southwest Forestry University. [In Chinese]
    22. He, J.M., Wei, R.P., Wang, R.H. et al. (2025) [Study on the leaf trait variation of Michelia chapensis clones.] Journal of Southwest Forestry University, 45, 32-37. [In Chinese]
    23. Heath, R.L. & Packer, L. (1968) Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125, 189-198. Go to original source...
    24. Huang, W.L., Zhou, T.T., Xue, L., Li, J. & Lie, Z.Y. (2016) Density effect on physiological characteristics in Michelia chapensis seedlings. In: Ding, H.B. (Ed.) Food Hygiene, Agriculture and Animal Science. Proceedings of the 2015 International Conference on Food Hygiene, Agriculture and Animal Science. Singapore: World Scientific, pp. 270-276. Go to original source...
    25. Huchzermeyer, B., Menghani, E., Khardia, P. & Shilu, A. (2022) Metabolic pathway of natural antioxidants, antioxidant enzymes and ROS providence. Antioxidants, 11, 761. Go to original source...
    26. Huyen, P.V., Hien, N.T.T., Hanh, T.T.N., Duyen, N.H.H., Thuan, N.T.D. & Phan, N.H.T. (2025) Phytochemistry and biological activities of Magnolia genus: from 2003 to 2023. Phytochemistry Reviews. Go to original source...
    27. Irato, P. & Santovito, G. (2021) Enzymatic and non-enzymatic molecules with antioxidant function. Antioxidants, 10, 579. Go to original source...
    28. Jiang, J.M. (2006) [Genetic resource evaluation of the Magnoliaceae species and cultivar breeding of Michelia chapensis Dandy.] PhD Thesis. Beijing: Chinese Academy of Forestry. [In Chinese]
    29. Jiao, L., Yang, R., Zhang, Y., Yin, J. & Huang, J. (2022) The evolution and determinants of ecosystem services in Guizhou - a typical karst mountainous area in southwest China. Land, 11, 1164. Go to original source...
    30. Latocha, P., Wo³osiak, R., Worobiej, E. & Krupa, T. (2013) Clonal differences in antioxidant activity and bioactive constituents of hardy kiwifruit (Actinidia arguta) and its year-to-year variability. Journal of the Science of Food and Agriculture, 93, 1412-1419. Go to original source...
    31. Li, Y., Zhang, S., Jiang, W. & Liu, D. (2013) Cadmium accumulation, activities of antioxidant enzymes, and malondialdehyde (MDA) content in Pistia stratiotes L. Environmental Science and Pollution Research, 20, 1117-1123. Go to original source...
    32. Liao, T., Cao, J., Yang, Z., Cheng, J. & Lu, J. (2022) Leaf and flower extracts of six Michelia L.: polyphenolic composition, antioxidant, antibacterial activities and in vitro inhibition of α-amylase and α-glucosidase. Chemistry & Biodiversity, 19, e202100894. Go to original source...
    33. Liu, H., Zhu, L., Xu, Q. et al. (2018) Ecophysiological responses of two closely related Magnoliaceae genera to seasonal changes in subtropical China. Journal of Plant Ecology, 11, 434-444. Go to original source...
    34. Llauradó Maury, G., Méndez Rodríguez, D., Hendrix, S. et al. (2020) Antioxidants in plants: a valorization potential emphasizing the need for the conservation of plant biodiversity in Cuba. Antioxidants, 9, 1048. Go to original source...
    35. McCord, J.M. & Fridovich, I. (1969) Superoxide dismutase: an enzymic function for erythrocuprein (hemocuprein). Journal of Biological Chemistry, 244, 6049-6055. Go to original source...
    36. Melgarejo-Sánchez, P., Martínez, J.J., Legua, P., Martínez, R., Hernández, F. & Melgarejo P. (2015) Quality, antioxidant activity and total phenols of six Spanish pomegranates clones. Scientia Horticulturae, 182, 65-72. Go to original source...
    37. Mnich, E., Bjarnholt, N., Eudes, A. et al. (2020) Phenolic cross-links: building and de-constructing the plant cell wall. Natural Product Reports, 37, 919-961. Go to original source...
    38. Morales, M. & Munné-Bosch, S. (2019) Malondialdehyde: facts and artifacts. Plant physiology, 180, 1246-1250. Go to original source...
    39. O'Dell, R.E. & Rajakaruna, N. (2011) Intraspecific variation, adaptation, and evolution. In: Harrison, S. (Ed.) Serpentine: The Evolution and Ecology of a Model System. Los Angeles: University of California Press, pp. 96-137. Go to original source...
    40. Pan, L., Ye, L., Chen, H. & Lie, G. (2021) Visible symptoms and changes in physiological parameters of Cinnamomum camphora and Michelia chapensis under ozone stress. International Journal of Environment and Pollution, 70, 10-21. Go to original source...
    41. Pan, L., Zou, X.-J., Lie, G.-W., Xue, L. & Chen, H.-Y. (2020) Ozone-induced changes in physiological and biochemical traits in Elaeocarpus sylvestris and Michelia chapensis in South China. Atmospheric Pollution Research, 11, 973-980. Go to original source...
    42. Rajput, V.D., Harish, Singh, R.K. et al. (2021) Recent developments in enzymatic antioxidant defence mechanism in plants with special reference to abiotic stress. Biology, 10, 267. Go to original source...
    43. Saed-Moucheshi, A., Sohrabi, F., Fasihfar, E., Baniasadi, F., Riasat, M. & Mozafari, A.A. (2021) Superoxide dismutase (SOD) as a selection criterion for triticale grain yield under drought stress: a comprehensive study on genomics and expression profiling, bioinformatics, heritability, and phenotypic variability. BMC Plant Biology, 21, 148. Go to original source...
    44. Shen, C., Chen, X., Zhou, C. et al. (2025) Predicting suitable spatial distribution areas for urban trees under climate change scenarios using species distribution models: a case study of Michelia chapensis. Land, 14, 638. Go to original source...
    45. Shen, X.Y. (2020) [Comprehensive evaluation on salt tolerance of Magnolia denudata, Magnolia biondi, and Michelia chapensis seedlings.] Master Thesis. Hangzhou: Zhejiang A&F University. [In Chinese]
    46. Shen, X.Y., Jin, H.X., Chen, R.R., Shen, Y.M. & Zhang, L. (2020) [Effect of NaCl stress on some physiological indexes of leaves of seedlings of three species in Magnoliaceae.] Journal of Plant Resources and Environment, 29, 75-77. [In Chinese]
    47. Sima, Y., Li, Y., Yuan, X. & Wang, Y. (2020) The complete chloroplast genome sequence of Michelia chapensis Dandy: an endangered species in China. Mitochondrial DNA Part B, 5, 1594-1595. Go to original source...
    48. Smirnoff, N. & Arnaud, D. (2019) Hydrogen peroxide metabolism and functions in plants. New Phytologist, 221, 1197-1214. Go to original source...
    49. Song, M., Fan, S., Pang, C., Wei, H. & Yu, S. (2014) Genetic analysis of the antioxidant enzymes, methane dicarboxylic aldehyde (MDA) and chlorophyll content in leaves of the short season cotton (Gossypium hirsutum L.). Euphytica, 198, 153-162. Go to original source...
    50. Szabados, L. & Savouré, A. (2010) Proline: a multifunctional amino acid. Trends in Plant Science, 15, 89-97. Go to original source...
    51. Vuksanoviæ, V., Kovaèeviæ, B., Kebert, M. et al. (2023) In vitro selection of drought-tolerant white poplar clones based on antioxidant activities and osmoprotectant content. Frontiers in Plant Science, 14, 1280794. Go to original source...
    52. Vuleta, A., Jovanoviæ, S.M. & Tuciæ, B. (2016) Adaptive flexibility of enzymatic antioxidants SOD, APX and CAT to high light stress: The clonal perennial monocot Iris pumila as a study case. Plant Physiology and Biochemistry, 100, 166-173. Go to original source...
    53. Wang, J., Ren, H., Yang, L. & Duan, W. (2009) Establishment and early growth of introduced indigenous tree species in typical plantations and shrubland in South China. Forest Ecology and Management, 258, 1293-1300. Go to original source...
    54. Wang, Z.-L., Wu, D., Hui, M. et al. (2022) Screening of cold hardiness-related indexes and establishment of a comprehensive evaluation method for grapevines (V. vinifera). Frontiers in Plant Science, 13, 1014330. Go to original source...
    55. Wei R.P., Yan S., Liang S.M. et al. (2025) [Division of suitable distribution of Michelia chapensis in China based on current climate conditions.] Chinese Wild Plant Resources, 44, 97-105. [In Chinese]
    56. Yoshida, K., Kaothien, P., Matsui, T., Kawaoka, A. & Shinmyo, A. (2003) Molecular biology and application of plant peroxidase genes. Applied Microbiology and Biotechnology, 60, 665-670. Go to original source...
    57. Zhou, W., Duan, Y., Jiang, X. et al. (2023) Transcriptome and metabolome analyses reveal novel insights into the seed germination of Michelia chapensis, an endangered species in China. Plant Science, 328, 111568. Go to original source...

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