biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 70:47-55, 2026 | DOI: 10.32615/bp.2026.006

Photosynthetic dysfunction and antioxidant defense disruption in rice seedlings induced by tire wear microplastics

Zailin ZHANG, Dongyi WANG, Zhangtianxiao LI, Chaoyang WEI, Chuanling ZHANG*, Liyan YIN*
School of Life and Health Sciences, Hainan Province Key Laboratory of One Health, Collaborative Innovation Center of Life and Health, Hainan University, Haikou, Hainan 570228, P.R. China

Background and aims: Tire wear microplastics (TWMs) are emerging environmental contaminants, but their ecological risks to agricultural systems remain poorly understood.

Methods: Rice seedlings were exposed to 0, 10, 100, and 1 000 mg L-1 TWMs for 10 days. Growth parameters, chlorophyll fluorescence, and antioxidant enzyme activities were measured.

Results: TWMs promoted growth; exposure to 1 000 mg L-1 TWMs increased plant height and root length by 8.06% and 57.38%, respectively. Chlorophyll fluorescence analysis revealed that TWMs significantly suppressed rETRmax by 32.53 - 43.62% and altered qP and NPQ. TWMs inhibited Y(NPQ) while enhancing Y(NO) loss, indicating impaired photoprotective dissipation and aggravated photodamage. TWMs also inhibited SOD, POD, CAT, and APX activities in both leaves and roots, with root CAT and APX decreasing by up to 37.35% and 40.34%, reflecting a direct impairment of the antioxidant defense system.

Conclusions: Rice seedlings achieve TWM-induced short-term growth at the expense of compromised photosynthetic efficiency and antioxidant defense, leading to an unsustainable compensatory state. This study provides physiological evidence for assessing TWMs phytotoxicity in agricultural systems.

Keywords: antioxidant system, chlorophyll fluorescence, photosynthesis, rice, tire wear microplastics.

Received: December 15, 2025; Revised: March 6, 2026; Accepted: March 20, 2026; Published online: April 7, 2026  Show citation

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ZHANG, Z., WANG, D., Zhangtianxiao, L., WEI, C., ZHANG, C., & YIN, L. (2026). Photosynthetic dysfunction and antioxidant defense disruption in rice seedlings induced by tire wear microplastics. Biologia plantarum, 70, Article 47-55. https://doi.org/10.32615/bp.2026.006
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