biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 70:56-64, 2026 | DOI: 10.32615/bp.2026.007

Rapid in vitro propagation of elite female plants of Idesia polycarpa var. vestita Diels

Feng Ying CAO1, Ting XIE1, Jie LI1, *, Yong Bin OU1, Fu Rong LIU2, Jian Xun LUO2
1 Engineering Research Center of Biomass Materials, Ministry of Education, College of Life Sciences and Agri-Forestry, Southwest University of Science and Technology, Mianyang, Sichuan 621010, P.R. China
2 Sichuan Academy of Forestry, Chengdu, Sichuan 610084, P.R. China

Background: Idesia polycarpa var. vestita Diels is an oil-bearing woody plant of significant economic value.

Aims: To accelerate the propagation of its elite germplasm, this study aimed to establish an efficient and stable in vitro rapid propagation system using one-year-old stem segments from elite plants.

Methods: Young stem explants were disinfected with 75% ethanol for 40 s, followed by 0.1% HgCl2 for 6 min. MS or 1/2 MS served as basal media, and various combinations of plant growth regulators were tested for axillary bud induction, proliferation, and rooting.

Results: The optimal disinfection protocol yielded a 38.10% survival rate. The best axillary bud induction medium was MS + 1.5 mg/L 6-BA + 0.05 mg/L NAA + 0.08 mg/L TDZ, achieving 100% induction. For proliferation, MS + 1.5 mg/L 6-BA + 0.06 mg/L NAA + 0.01 mg/L TDZ (proliferation coefficient 5.81) was optimal. Rooting was best with 1/2 MS + 0.3 mg/L IAA + 0.1 mg/L NAA (100% rooting, 14 roots/plant).

Conclusions: This study established a reliable micropropagation system for Idesia polycarpa var. vestita, providing technical support for the efficient production of high-quality seedlings.

Keywords: Idesia polycarpa var. vestita Diels, plant regulator, tissue culture.

Received: January 19, 2026; Revised: March 6, 2026; Accepted: March 31, 2026; Published online: April 21, 2026  Show citation

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Ying CAO, F., XIE, T., Jie, L., Bin OU, Y., Rong LIU, F., & LUO, J.X. (2026). Rapid in vitro propagation of elite female plants of Idesia polycarpa var. vestita Diels. Biologia plantarum, 70, Article 56-64. https://doi.org/10.32615/bp.2026.007
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