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Interaction of morphactin with gibberellie acid in whole plants and by rooting of cuttings.E. KrelleBiologia plantarum 12:256-264, 1970 | DOI: 10.1007/BF02920810 The methyl-ester of 2-chloro-9-hydroxyfluorenole-9-carboxylic acid (Chlorflurenol-Methyl =CFM) enhances the elongation of intact plants ofColeus blumei and induces leaf curvatures and an anormalous abscission of the youngest leaves. In long-time-experiments (3-4 month), CFM induces increased outgrowth of the axillary buds including leaf deformations and abscission of organs on the originating shoots. CFM stimulates the adventitious root formation of cuttings fromHelianthus, Phaseolus, andColeus. It is shown inColeus cuttings that an inhibition of root formation precedes the stimulation of root formation. Gibberellic acid does not influence any one of CFM-induced effects. Therefore morphactins are not GA3-antagonists. TIBA qualitatively causes the same effects as CFM. Therefore it is possible that the mechanisms of actions of both substances are similar. |
Biogenesis of indole compounds from D- and L-tryptophan in segments of etiolated seedlings of cabbage, maize and peaM. Kutáček, V. KefeliBiologia plantarum 12:145-158, 1970 | DOI: 10.1007/BF02920863 The metabolism of D-and L-tryptophan-3-14C (Try-3-14C) was studied and compared for three different plant species, cabbage, maize and pea. Apical segments of the seedlings were incubated for 6 hours in solutions of L- or D-Try-3-14C (1.5 μc/ml) with the addition of chloramphenicol (10-4g/ml) and then allowed to stand for another 20 hours in moist chambers. The methanolic extract of the tissues was analyzed radiochromatographically and by paper electrophoresis in combination with biological tests. Chloramphenicol in a concentration of 10-4 g/ml had little influence on the growth of the segments, though the antibiotic slightly decreased the uptake of L-Try, it did not prevent the formation of IAA from L-Try. In the segments of cabbage the following metabolites were formed from L-Try-3-14C (accounting for 52% of the activity of the chromatographically separated extract): glucobrassicin (26.0%), neoglucobrassicin (3.6%), a spot corresponding according to its Rf to 3-indolylacetamide (IAAmide-10.9%), β-glucoside of 3-indolylacetic acid (IAGluc-3.3%) and traces of 3-indolylacetonitrile (IAN), IAA and indole-3-carboxylic acid (total 5%). In maize segments L-Try-3-14C (53.0%) was transformed to several unidentified hydrophilic substances, one of them possessing auxin activity (total amount 6.9%), IAGlue (9.3%) accompanied by a small amount of tryptamine, a spot corresponding according to its Rf to IAAmide (16.5%), IAA and another unidentified hydrophobic substance (4.1%). In pea segments L-Try-3-14C (66.7%) gave a zone corresponding according to its Rf to IAAmide (20.0%), a substance similar to IAGluc (10.5%) and also hydrophobic substances (3.1%) containing traces of IAA, which could be demonstrated only by bioassay. |
Changes in content of sugars and their exosmose from maize kernels in relation to cold resistanceEliska Vedralová, V. Sege»aBiologia plantarum 12:265-274, 1970 | DOI: 10.1007/BF02920811 Changes in soluble sugar content in individual parts (embryo, endosperm and pericarp) of maize caryopses exposed to the influence of 24°, 10° and 6° were studied. At the same time the eluates from sand in which the seeds were planted and exposed for various periods were analysed by paper chromatography. |
Application of growth analysis to potatoes in field culture and some specific features of potato growthJosef NečasBiologia plantarum 7:180-193, 1965 | DOI: 10.1007/BF02921454 In field experiments, potato varieties with different lengths of the vegetation period were compared. The methods of growth analysis were applied to the evaluation of these experiments. Some important specific features of potato growth had to be taken into account to make the application of the growth analysis successful and to enable valid and reliable results to be obtained. |
Content of phenolic compounds and lignification of the woody parts of apple shoots depending on mineral nutritionH. Miidla, A. Milius, T. VainjärvBiologia plantarum 12:11-18, 1970 | DOI: 10.1007/BF02920874 The paper deals with the effect of mineral nutrition on the content of free flavonoid glycosides (phloridzin, hyperine) free and bound C6-C1 aldehydes (vanillin, syringic aldehyde) in the woody parts of apple shoots of the variety 'Antonovka' and on the content of products oxidized by nitrobenzene (vanillin, syringic aldehyde) in connection with the lignification of cell walls. |
Effect of β-indoleacetic acid, maleic hydrazide, and 2,3,5-triiodobenzoic acid on N, P, K, and Ca accumulation by pea plantsAlena Raková, J. MinarBiologia plantarum 12:348-358, 1970 | DOI: 10.1007/BF02922189 A study was performed on the effect of various concentrations of IAA, 2,3,6-triiodobenzoic acid, and maleic hydrazide, supplied to Richter's nutrient solution, on growth of pea plants in water cultures. After a 18-day cultivation growth was evaluated and in the plants gathered the content of total N, P, K, and Ca was estimated. |
Symmetry in thePisum seedling and growth regulatorsRudolf DostálBiologia plantarum 7:308-317, 1965 | DOI: 10.1007/BF02921439 The rather equal development of the two cotyledons determines the bilateral symmetry in the pea seedling, just as their unilateral position results in the dorsiventrality of the epicotyl and root. The morphogenic manifestation of the symmetry relations conditioned, in the first place, by a different distribution of auxin out of the cotyledons may be enhanced by means of exogenous growth regulators, particularly IAA, GA3, and TIBA. |
Testing of production properties of algal clones in greater setsJ. NečasBiologia plantarum 12:19-30, 1970 | DOI: 10.1007/BF02920875 The author describes the testing method for clonal selections from the original strainScenedesmus quadricaudaGreifswald/15 in biomass production. This method served for testing the clones in greater series. It was applied to clonal cultures selected on a solid medium after treatment of the coenobia with UV-light before their inoculation. The production characteristic of the clones was expressed by the temperature dependence of the constant μ from Simmer's growth equation in the last step of the testing process used. Some significant differences of this kind were found among the evaluated clonal cultures. This way of characterization facilitates a more detailed evaluation of clones in comparison with values obtained by an oneshot testing method under standard conditions. |
Influence of natural substances of phenolic character and diethyldithiocarbamate on the metabolism of L-tryptophan in cabbage, maize and peaV. Kefeli, M. Kutáček, Květa VackováBiologia plantarum 12:81-90, 1970 | DOI: 10.1007/BF02920854 The effect of phenolic substances isolated earlier from cabbage, maize and pea on L-tryptophan-3-14G (L-Try-14C) metabolism in those plants was investigated. For the sake of comparison the effect of diethyldithiocarbamate (DIECA) on cabbage was also observed. A phenolic substance of unknown structure isolated from cabbage was utilised in a 0.5 mg/ml concentration, p-coumarie acid (PCA) from maize in 0.7 mg/ml concentration and quercetin-glucosyl-coumarate (QGC) found in pea in 8 mg/ml concentration were used. The chosen concentrations were on the limit of their inhibitory effect on the growth of the respective plant apical segments. The effect of DIECA was investigated at 0.1 mg/ml concentration. Using non-labelled L-Try as substrate (5 × 10-3 M) biological tests have shown that QGC and DIECA have a weaker but distinct effect on the increase in auxin level (+ 17% and + 15%, respectively). All phenolic compounds, as well as DIECA increase the intake of L-Try-14C from the incubation solutions. Phenolic substances decrease the conversion of L-Try-14C to its metabolites. The changes were studied after a 20 hour period of L-Try-14C metabolism. On chromatograms changes in the proportion of L-Try-14C metabolites took place, especially in the areas of substances of complex character from which IAA splits off easily,i.e. in the areas of 3-indolylacetylglucose and 3-indolylaeetamide. The radioactivity of these areas is generally decreased (e.g. the decrease of radioactivity in the 3-indolylacetylglucose area under the influence of the phenolic substance ofBrassica plants is -7,4%, under the influence of PCA in maize it is -8.9% and under the influence of QGC in pea it is -17.1% DIECA also decreased the radioactivity of this zone, by -10,5%. In cabbage a large part of L-Try-14C was transformed to glucobrassicin; its biosynthesis increases in the presence of the phenolic substance of cabbage by +3%, in the presence of DIECA by +27%. The results are discussed in a working hypothesis based on the key formation of IAA, accompanied by its oxidation and detoxication. |
Water balance of plants during root application of high concentrations of growth substancesV. KozinkaBiologia plantarum 10:398, 1968 | DOI: 10.1007/BF02920983 Using hydroponic cultures, the effect of high concentrations (10-3 m) of 2-methyl-4-chlorophenoxyacetic acid (MCPA) in the root medium on the water balance of 8-9 week old plants ofPisum sativum L. and of 9-10 week-old plants ofSinapis alba L. was studied. The water balance was determined in the light and in the dark gravimetrically by measuring the intensity of water uptake and transpiration in plants cultivated by the method of root bridges according to Werner. MCPA present in the root medium in illuminated plants decreased rapidly the intensity of both the uptake and the loss components of the water balance. In permanent darkness, MCPA brought about an increase in the intensity of uptake of water and of transpiration. Simultaneous determination of water uptake and transpiration showed that the intensity of transpiration remained higher than the intensity of water uptake. This indicates that in the presence of MCPA in the root medium the relationship between the uptake and the loss components of water balance is not quantitatively equal, enboth in the light and in the dark. The existing disproportion results in the formation of a passive water balance of plants. |
Spiral feedback oscillations of growing hypocotyl with radicle inPisum sativum L.M. SpurnýBiologia plantarum 8:381, 1966 | DOI: 10.1007/BF02930674 Cinematographic records of longitudinal growth showed that hypocotyl with radicle inPisum sativum L. undergoes spiral oscillations during growth. This phenomenon can be characterized by the following time-space limits: |
Course of growth and development of Spring BarleyP. Strebeyko, B. GóraBiologia plantarum 6:152, 1964 | DOI: 10.1007/BF02926670 The barley variety "Browarny P.Z.H.R." was grown in pots. Experiments on the course of development were conducted for two years. The increase in the dry weight of roots, leaves, stems and grain was recorded. The assimilation area of the plants was measured periodically and the rate of respiration in detached leaves was determined. Photographic records were kept of growing point and ear development. The dynamics of growth and development were studied during the whole vegetation period. The present results are similar to those previously obtained for spring wheat. The short period of leaf activity is the chief factor limiting barley growth. |
The participation of flavine enzymes in the respiration of root zones of the horse bean (Vicia faba L.).Věra Hadačová, M. DvořákBiologia plantarum 11:450, 1969 | DOI: 10.1007/BF02920709 As part of our studies on respiration systems in root zones of the horse bean (Vicia faba L., cv. Chlumecký) the influence of quininehydrochloride and potassium cyanide on respiration was followed. The inhibition of the oxygen uptake by quininehydrochloride was highest in the elongation zone and lowest in the meristematic zone. Thes shows that the flavine enzyme content is lowest in the meristem, highest in the elongation zone. The inhibition of Fe- and Cu-enzymes by KCN increases from the root tip towards the older parts of the root, but is lower than the inhibition by quininehydrochloride. The difference between the intensity of action of both, applied separately, and of the combined effect of both if compared with the action of cyanide alone, is highest in the elongation zone; this shows that relatively higher amounts of non-metallic flavine enzymes participate in this zone. Their relation to the pentose phosphate cycle is discussed. |
Influence of light and darkness on the concentration of lactic, glycolic, succinic, malic and citric acid in pea plantsJana Barthová, Sylva LeblováBiologia plantarum 11:97-109, 1969 | DOI: 10.1007/BF02921728 Chromatographic separation of an extract of organic acids on a Dowex-l column in the formiate cycle was used to study the content of several organic acids in pea plants, cultivated either in light or in darkness. Concentration changes of the individual acids in the course of growth indicate that the citrate cycle is blocked in the cotyledons of plants grown in light in the period around the 15th day of growth, probably at the site of succinic dehydrogenase (succinic and lactic acids accumulate and the content of citric and malic acids is exhausted). There is no inhibition in the cotyledons of etiolated plants. In vegetative organs, the concentration of the majority of the acids studied is lower than in cotyledons, probably because synthetic processes prevail over degradation processes in these organs. It seems that other processes besides the citrate cycle participate in malate synthesis in pea plants. |
Effect of carbohydrates on the growth of Rhizoctonia solani KühnO. P. Israel, Md. Shah AliBiologia plantarum 6:84-87, 1964 | DOI: 10.1007/BF02926661 The growth OfRhizoctonia solani in different carbohydrates was studied. The rate of growth of the fungus was traced by taking the dry weights of mycelia obtained from the carbohydrate medium at regular intervals and shifts in the pH were recorded. Different carbohydrate sources had different effects on the growth of the organism. The exoenzymes from the organism were capable of cleaving carbohydrates irrespective of whether the fungus grew in them or not. |
Mycoplasma-like bodies in plants infected with potato witches' broom disease and the response of plants to tetracycline treatmentJ. Brčák, O. Králík, J. Limberk, Marie UlrychováBiologia plantarum 11:470, 1969 | DOI: 10.1007/BF02920712 The virus origin of a Czechoslovak isolate of potato witches' broom disease is discounted: electron micrographs of ultrathin section of phloem tissues from plants infected with potato witches' broom disease demonstratedMycoplasma-like bodies, spherical or elongated showing binary fission and fragmentation. The minute corpuscles have a diameter of about 50-60 nm, the largest bodies of 1000 nm. The width of elongated filamentous structures is about 200 nm, most oval bodies have a diameter of 250 nm. A weak tetracycline treatment of diseased plants causes a delay of symptom development; a strong dose of tetracycline (applied by means of the wick method into the stem) inhibits symptom appearance completely. Tetracycline produces a phytotoxic effect inhibiting the growth of tomato plants and causing (at higher concentrations) necrosis and death of these plants. There is a note in the paper dealing with the term "mycoplasma". The word mycoplasma in the sense ofEriksson (1897) or ofMereschkowsky (1910) does not correspond to the genus nameMycoplasma Nowak (1929). |
Effect of trophic conditions on asparagine transamination in wheat plantsAlena ČinčerováBiologia plantarum 11:139, 1969 | DOI: 10.1007/BF02921732 In dialyzed extracts from winter wheat plants transamination reactions occurred between asparagine and α-ketoglutaric acid (L-asparagine+2-oxoacid=2-oxosuccinamate+ +amino acid; 2. 6. 1. 14). Reactions with pyruvate exhibited a very low activity. Besides transamination products,i. e. glutamate and alanine, aspartic acid was formed in both reactions. Deamidation was more intensive in the weak reaction asparagine-alanine and less intensive in the asparagine-glutamate reaction. |
Glucobrassicin a potential inhibitor of unusual type affecting the Germination and growth of plants; mechanism of its actionMilan KutáčekBiologia plantarum 6:88-98, 1964 | DOI: 10.1007/BF02926662 A water-soluble germination- and growth-inhibitor present in plants of Brassica genus was studied. Aqueous eluates from chromatograms of methanolic extracts of Savoy cabbage, cabbage and Brussels' sprouts leaves, and of Brussels' sprouts roots, inhibited the growth of wheat; the chromatographic behaviour of this inhibitor was similar to that of the thioglycoside glucobrassicin (GLUBR). GLUBRat 10-3 m, and the supposed product of its enzymatic degradation, 3-indolylacetonitrile (IAN) at 10-4 m, inhibited the growth of wheat and clover in a similar manner. At lower concentrations, both substances gradually enhanced growth. In contrast to IAN, GLUBR did not appreciably influence the growth of Savoy cabbage. Other products of GLUBR degradation, such as thiocyanate ion and ascorbigen, influenced the growth to a lesser extent and in a different way. At higher concentrations GLUBR and IAN also inhibited the germination of clover seeds. The biological effect of GLUBR is dependent on the presence of the indole nucleus in its molecule; the thioglycoside glucoiberin, which is not of indolic character, does not influence growth. The presence of free myrosinase was shown in intact wheat seedlings. Hence, GLUBR taken in by the germinating plants must undergo enzymatic cleavage. The in vitro activity of myrosinase in wheat and clover was lower than in Savoy cabbage, but the quantity of IAN formed at a pH corresponding to the natural pH was higher in wheat and clover than in Savoy cabbage, where the formation of the growth-inactive ascorbigen prevailed. On the basis of these results the hypothesis was formulated that GLUBR, the indolic complex from the generaBrassica, Raphanus, Cochleria, Nasturtium, Barbarea andSisymbrium, is a representative of a class of potential inhibitors of growth and germination, whose activity can be explained by the liberation of large amounts of growth-affecting substances during their specific enzymatic cleavage. |
Endogenous auxins in apical buds ofChenopodium rubrum L. after application of (2-chloroethyl) trimethylammonium chloride (CCC)Lola TeltscherováBiologia plantarum 11:158, 1969 | DOI: 10.1007/BF02921734 Apical buds ofChenopodium rubrum from plants treated with CCC contain more endogenous auxins than buds from control plants, the level of these compounds increasing with the application of rising concentrations of the retardant. An especially marked increase was observed in the level of substance "X" which on chromatographic separation runs in the zone of tryptamine or its derivative. Since it has been shown in previous experiments that the inhibitory effect of CCC on flowering ofChenopodium rubrum may be reversed by indole-3-acetic acid (IAA) it is believed that the increase in auxins after application of CCC does not concern biologically active substances immediately available to the plant. It seems more likely that inactive precursors are involved which cannot be converted to the active substance in the presence of CCC, possibly due to blocking of the pertinent enzyme. If we assume that the wheat coleoptile used in the auxin bioassay in our experiments contains the pertinent enzyme it might convert the inactive precursors to active substances and, therefore, exhibit a growth stimulation even though the substances concerned would not necessarily be active in the buds from which they were extracted. |
Influence of sodium humate and nutritive conditions on the content of nucleic acids, particularly on the ribosomal ribonucleic acid in wheat rootsSvatava FialováBiologia plantarum 11:8, 1969 | DOI: 10.1007/BF02920726 Changes in the nucleic acid (NA) content were studied in roots of young wheat plants cultivated under various nutritive conditions, namely in a nutrient solution, in distilled water and in a solution of sodium humate in distilled water. Changes in the ribosomal ribonucleic acid (RNA) in particular and their connection with growth rate were investigated. The amount of the NA fraction investigated (more than 90 per cent of which is represented by the ribosomal RNA) changed substantially under the cultivation conditions studied. In roots of one plant cultivated in water the content of the NA fraction investigated was at the most about 25 μg and it began to decrease as early as from the second day of cultivation. After 12 days of cultivation it decreased to 15 μg. When cultivated in Na-humate the roots contained at the most 33 μg NA, between the 5th-7th day, followed by an intensive decrease after 12 days, to 17 μg. The content of the fraction investigated in wheat plants cultivated in a nutrient solution was about double the value in comparison to these two cases with the maximal value about 60 μg between the 7th-9th day. After 12 days this amount decreased to 45 μg. |
Effects of shoot bending of apple trees on accumulation and translocation of14C-labelled assimilatesA. MikaBiologia plantarum 11:175, 1969 | DOI: 10.1007/BF02921736 The translocation of14C-labelled assimilates from a single leaf in bent and intact apple shoots was studied in varying stages of shoot development. In actively growing shoots14C-labelled assimilates translocated from the treated leaf and accumulated mainly in the shoot apex. In moderately growing apple shoots radioactive assimilates were translocated from the treated leaf in both directions towards and down the shoot. In apple shoots showing only slight growth activity the14C-labelled assimilates were transported from the treated leaf mainly to the base of the shoot, stem and roots. Bending shoots changed the pattern of distribution of radioactive assimilates. Bending actively and moderately growing shoots resulted in higher concentration of 14-carbon in the shoot apex than in controls. In slowly growing and non-growing apple shoots bending caused a higher accumulation of radioactive assimilates in the bent section than in an equal section of control shoots. |
Metabolism of nucleic acids in wheat roots in dependence on nutritive conditionsSvatava FialováBiologia plantarum 11:424, 1969 | DOI: 10.1007/BF02920706 Influence on individual types (fractions) of nucleic acids (NA) was studied in roots of wheat plants grown in various cultivation media, namely in distilled water, in sodium humate and in a nutrient solution. NA's were prepared by means of the phenol technique. Using separation on a methylalbumine column (MAK) five fractions were obtained, namely: fraction I.- low molecular weight substances, fraction II.-soluble RNA, fraction III.-DNA-RNA, and the ribosomal RNA in two fractions, IV.-(l r-RNA) and V.-(h r-RNA). Of the NA fractions investigated, the r-RNA fraction was noticeably influenced by the kind of nutrition, its amount varying in a certain proportion to the growth intensity affected by the cultivation medium. The other NA fractions were not apparently affected. |
Integrant characteristics in the deflexion of biserial buds in pea seedlingsR. DostálBiologia plantarum 11:193, 1969 | DOI: 10.1007/BF02920797 The main epicotyledonary axis inPisum sativum exhibits a very fluctuating either left- or right-handed deflexion of its first-order laterals, but the biserial buds on the laterals of various further order reveal in this respect certain regularities according to their position on the seedling, their deflexion being very often adaxial in the vicinity of cotyledons, however, in more distant and therefore later produced laterals, as a rule, abaxial. The integrant characteristics of these relationships may become clear from their experimental shift with the aid of exogenous auxin increasing inhibitions as well as of triiodobenzoic acid blocking the transport of endogenous auxin participant also in these embryonic-growth correlations. |
Effect of different constant soil moisture levels on foliage development in maizeJ. VáclavíkBiologia plantarum 11:68, 1969 | DOI: 10.1007/BF02920732 The effects of constant soil moisture levels of 90, 60 and 40% of the maximum capillary capacity, applied beginning from the planting of the germinated kernels, on the dynamics of the foliage development of maize was studied in relation to ontogenical changes in leaf area of individual leaves. There were two maxima in the growth of total leaf area (characterized by leaf area duration and loaf area relative growth rate) unrelated to the soil mositure. The first maximum which was less marked, appeared in the phase of 5-8 leaves, the second and main increase being observed before flowering in the phase of 10-12 leaves. The effects of continued decrease in soil moisture were a correspondingly progressive reduction in leaf area and delayed development of the whole plants. Plants with 60% soil moisture attained the same leaf area as those with 90% but only after the end of the vegetative phase. The greatest differences in rate of development between watering treatments appeared at the time of greatest increase in leaf area of control plants. Continued decrease in soil moisture markedly affected the development of the leaves at different insertion levels (especially in elder leaves). Clear-cut prolongation of ontogenesis took place under dry conditions. In conditions of higher soil moisture growth was rather limited or stopped after reaching a certain maximum. |
Cytogenetic studies on the effect of chronic gamma irradiation onVicia fabaSoheir M. Amer, I. Y. Mostafa, Evon MikhaelBiologia plantarum 11:432-441, 1969 | DOI: 10.1007/BF02920707 Vicia faba seeds (cv. Giza 1) were planted in the Inshas gamma radiation field where they were chronically irradiated during the whole life of the plant. The percentage of the induced abnormal P.M.Cs, as well as the frequency of abnormal P.M.Cs in the different meiotic stages were proportional with the given doses. The main types of chromosome aberrations were anaphase and telophase bridges, fragmentation and lagging chromosomes. The nearest plants to the source showed an inhibition of shoot growth, flower and seed sterility and irregular branching. At the dosage levels used irradiation had no effect on pollen fertility. |
Study of the effect of volatile substances from cereal rootsF. Plhák, Věra UrbánkováBiologia plantarum 11:226, 1969 | DOI: 10.1007/BF02920801 We traced the liberation and biological effect of volatile substances released from the roots of cereals,i. e. barley, wheat, rye and oats, on seedlings of the same and other plant species. Experiments were carried out in a closed glass apparatus with a static or circulating atmosphere in which the CO2 and O2 were permanently absorbed and supplemented, respectively. In some experiments the air was bubbled through water or through solutions of boric acid, barium hydroxide and potassium permanganate. |
Study of the interactions between cereals with respect to the soil nitrate nitrogenHana Hruąková, F. PlhákBiologia plantarum 11:347-355, 1969 | DOI: 10.1007/BF02921399 The interactions between cereals-wheat, barley, rye and oats-in combined cultivation in mixtures of always two species in relation 1:1 in the initial phase of growth were studied. During thirty days' cultivation in pots the growth of the experimental plants in pure cultures and in mixtures and the changes of the nitrate nitrogen content of the soil in the experimental vessels were followed. |
Growth of radish (Raphanus sativus) seedlings in magnetic fieldsM. H. SimmersBiologia plantarum 9:377-382, 1967 | DOI: 10.1007/BF02929781 Radish seedlings were grown in asymmetric magnetic fields. The number of seeds germinated and the dry weight of the plants were the two criteria by which possible effects were examined. Two experiments were done; in the first the plants were grown for an average of 7.7 days and in the second for 14.1 days. A statistical analysis of the results failed to reveal any significant difference between control plants grown in dummy magnets and those subjected to the magnetic influence. |
The effect of 5-azacytidine on the root meristem ofVicia fabaV. Fučík, Z. ©ormová, F. ©ormBiologia plantarum 7:58-64, 1965 | DOI: 10.1007/BF02920680 The primary roots ofVicia faba seedlings were placed in a solution of 5-azacytidine and their further growth was observed after being replaced in running tap water. No inhibition of elongation occurred during the action of the 10-5 M solution of 5-azacytidine for 24 hours, but during subsequent cultivation in water in the absence of inhibitor, further growth was blocked. This inhibition could be overcome by cytidine, uridine, sodium azide, 5-azidomethyluracil and simultaneously with the 5-azacytidine solution. Inhibition was accompanied by a high incidence of chromosome stickiness and to a less extent by an incidence of chromosome aberrations. The occurrence of stickiness and chromosome aberrations was prevented by adding excess cytidine to the 5-azacytidine solution. |
The influence of light, darkness and changes in co2 and o2 concentration in die atmosphere on growth and gas exchange in pea (Pisum sativum)Jana Barthová, Sylva Leblová, J. KoątířBiologia plantarum 9:173, 1967 | DOI: 10.1007/BF02929735 When compared with the control plants, the increase in dry weight in the growing parts of germinating plants ofPisum sativum L., cultivated in a closed atmosphere in darkness or light is heavily inhibited. The concentration of carbon dioxide in the closed atmosphere increased in 60 mg CO2/per 1 g of the final dry weight of plants after 10 days. Similar results were obtained with older plants under similar conditions. The Knop solution caused a slight increase of dry weight in the growing parts of plants, but did not change considerably the relations in gas exchange. The results show that even plants which are able to photosynthetize, are only transpiring under the above conditions; first, when the plants were devoid of cotyledons, as storage organs, the changes in their dry weight (but not the changes in the CO2 and O2 exchange in a closed atmosphere) seemed to show photosynthetic income of CO2 (while its concentration increased to 3-6 volume %) when compared with the control. |


