biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

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Results 1771 to 1800 of 1889:

Interaction of morphactin with gibberellie acid in whole plants and by rooting of cuttings.

E. Krelle

Biologia plantarum 12:256-264, 1970 | DOI: 10.1007/BF02920810

The methyl-ester of 2-chloro-9-hydroxyfluorenole-9-carboxylic acid (Chlorflurenol-Methyl =CFM) enhances the elongation of intact plants ofColeus blumei and induces leaf curvatures and an anormalous abscission of the youngest leaves. In long-time-experiments (3-4 month), CFM induces increased outgrowth of the axillary buds including leaf deformations and abscission of organs on the originating shoots. CFM stimulates the adventitious root formation of cuttings fromHelianthus, Phaseolus, andColeus. It is shown inColeus cuttings that an inhibition of root formation precedes the stimulation of root formation. Gibberellic acid does not influence any one of CFM-induced effects. Therefore morphactins are not GA3-antagonists. TIBA qualitatively causes the same effects as CFM. Therefore it is possible that the mechanisms of actions of both substances are similar.

Biogenesis of indole compounds from D- and L-tryptophan in segments of etiolated seedlings of cabbage, maize and pea

M. Kutáček, V. Kefeli

Biologia plantarum 12:145-158, 1970 | DOI: 10.1007/BF02920863

The metabolism of D-and L-tryptophan-3-14C (Try-3-14C) was studied and compared for three different plant species, cabbage, maize and pea. Apical segments of the seedlings were incubated for 6 hours in solutions of L- or D-Try-3-14C (1.5 μc/ml) with the addition of chloramphenicol (10-4g/ml) and then allowed to stand for another 20 hours in moist chambers. The methanolic extract of the tissues was analyzed radiochromatographically and by paper electrophoresis in combination with biological tests. Chloramphenicol in a concentration of 10-4 g/ml had little influence on the growth of the segments, though the antibiotic slightly decreased the uptake of L-Try, it did not prevent the formation of IAA from L-Try. In the segments of cabbage the following metabolites were formed from L-Try-3-14C (accounting for 52% of the activity of the chromatographically separated extract): glucobrassicin (26.0%), neoglucobrassicin (3.6%), a spot corresponding according to its Rf to 3-indolylacetamide (IAAmide-10.9%), β-glucoside of 3-indolylacetic acid (IAGluc-3.3%) and traces of 3-indolylacetonitrile (IAN), IAA and indole-3-carboxylic acid (total 5%). In maize segments L-Try-3-14C (53.0%) was transformed to several unidentified hydrophilic substances, one of them possessing auxin activity (total amount 6.9%), IAGlue (9.3%) accompanied by a small amount of tryptamine, a spot corresponding according to its Rf to IAAmide (16.5%), IAA and another unidentified hydrophobic substance (4.1%). In pea segments L-Try-3-14C (66.7%) gave a zone corresponding according to its Rf to IAAmide (20.0%), a substance similar to IAGluc (10.5%) and also hydrophobic substances (3.1%) containing traces of IAA, which could be demonstrated only by bioassay.
D-Try is metabolised in the three plants by the virtually exclusive formation of malonyltryptophan.

Changes in content of sugars and their exosmose from maize kernels in relation to cold resistance

Eliska Vedralová, V. Sege»a

Biologia plantarum 12:265-274, 1970 | DOI: 10.1007/BF02920811

Changes in soluble sugar content in individual parts (embryo, endosperm and pericarp) of maize caryopses exposed to the influence of 24°, 10° and 6° were studied. At the same time the eluates from sand in which the seeds were planted and exposed for various periods were analysed by paper chromatography.
It was found that the lower was the temperature of exposure the greater were the amounts of sucrose, glucose, raffinose and other sugars diffused from the caryopses into the medium before the start of germination. The exosmose course and the changes in sugar content in kernel tissues proved that the hydrolysis of storage polysaccharides is not inhibited by low temperatures but that under such conditions the use of products of hydrolysis for the growth of the embryo is inhibited.
It is concluded that not only surface parts of endosperm but also the embryo participates in exosmose. The relatively high sugar exosmose found from seeds germinated at low temperatures explains the well-known interaction of pathogenic and parasitic microorganism in cold resistance during emergence.

Application of growth analysis to potatoes in field culture and some specific features of potato growth

Josef Nečas

Biologia plantarum 7:180-193, 1965 | DOI: 10.1007/BF02921454

In field experiments, potato varieties with different lengths of the vegetation period were compared. The methods of growth analysis were applied to the evaluation of these experiments. Some important specific features of potato growth had to be taken into account to make the application of the growth analysis successful and to enable valid and reliable results to be obtained.

Content of phenolic compounds and lignification of the woody parts of apple shoots depending on mineral nutrition

H. Miidla, A. Milius, T. Vainjärv

Biologia plantarum 12:11-18, 1970 | DOI: 10.1007/BF02920874

The paper deals with the effect of mineral nutrition on the content of free flavonoid glycosides (phloridzin, hyperine) free and bound C6-C1 aldehydes (vanillin, syringic aldehyde) in the woody parts of apple shoots of the variety 'Antonovka' and on the content of products oxidized by nitrobenzene (vanillin, syringic aldehyde) in connection with the lignification of cell walls.
It was established that the biosynthesis of phenolic compounds is retarded under the influenco of nitrogenous fertilizer while the biosynthesis of proteins is activated, intensifying growth. Contrary to nitric fertilizer, phosphoric with potassic fertilizers promote formation of phenolic compounds and suppress protein synthesis and growth; at the same time phenylalanine is included in the biosynthesis of lignin, but not in the biosynthesis of proteins.

Effect of β-indoleacetic acid, maleic hydrazide, and 2,3,5-triiodobenzoic acid on N, P, K, and Ca accumulation by pea plants

Alena Raková, J. Minar

Biologia plantarum 12:348-358, 1970 | DOI: 10.1007/BF02922189

A study was performed on the effect of various concentrations of IAA, 2,3,6-triiodobenzoic acid, and maleic hydrazide, supplied to Richter's nutrient solution, on growth of pea plants in water cultures. After a 18-day cultivation growth was evaluated and in the plants gathered the content of total N, P, K, and Ca was estimated.
Growth of experimental plants (as evaluated from fresh and dry weight) was affected by all three regulators in dependence on the concentration used. It was stimulated by lower concentrations and inhibited by higher, the production of both fresh and dry weight of the root system being stimulated by all IAA concentrations used.
The ratio of root dry weight to that of the entire plant was markedly increased after application of IAA and 2,3,5-triiodobenzoic acid, whereas when applying maleic hydrazide it was only slightly increased in comparison with control.
Stimulation or inhibition of growth induced by IAA treatment was accompanied by an accordingly increased or decreased accumulation of N, P, K, and Ca. Thus their utilization did not change in comparison with control. On the other hand, both inhibitory and stimulatory effects of 2,3,5-triiodobenzoic acid and maleic hydrazide on growth were associated with a relatively lower accumulation of the elements in question, resulting in an increased utilization.
The distribution index of N, P, K, and Ca decreased with increasing concentrations of IAA, 2,3,5-triiodobenzoic acid and maleic hydrazide. Only the highest 2,3,5-triiodobenzoic acid and maleic hydrazide concentrations used brought about a more marked increase in the distribution index of potassium, simultaneously with a marked decrease in the distribution index of calcium.

Symmetry in thePisum seedling and growth regulators

Rudolf Dostál

Biologia plantarum 7:308-317, 1965 | DOI: 10.1007/BF02921439

The rather equal development of the two cotyledons determines the bilateral symmetry in the pea seedling, just as their unilateral position results in the dorsiventrality of the epicotyl and root. The morphogenic manifestation of the symmetry relations conditioned, in the first place, by a different distribution of auxin out of the cotyledons may be enhanced by means of exogenous growth regulators, particularly IAA, GA3, and TIBA.

Testing of production properties of algal clones in greater sets

J. Nečas

Biologia plantarum 12:19-30, 1970 | DOI: 10.1007/BF02920875

The author describes the testing method for clonal selections from the original strainScenedesmus quadricaudaGreifswald/15 in biomass production. This method served for testing the clones in greater series. It was applied to clonal cultures selected on a solid medium after treatment of the coenobia with UV-light before their inoculation. The production characteristic of the clones was expressed by the temperature dependence of the constant μ from Simmer's growth equation in the last step of the testing process used. Some significant differences of this kind were found among the evaluated clonal cultures. This way of characterization facilitates a more detailed evaluation of clones in comparison with values obtained by an oneshot testing method under standard conditions.

Influence of natural substances of phenolic character and diethyldithiocarbamate on the metabolism of L-tryptophan in cabbage, maize and pea

V. Kefeli, M. Kutáček, Květa Vacková

Biologia plantarum 12:81-90, 1970 | DOI: 10.1007/BF02920854

The effect of phenolic substances isolated earlier from cabbage, maize and pea on L-tryptophan-3-14G (L-Try-14C) metabolism in those plants was investigated. For the sake of comparison the effect of diethyldithiocarbamate (DIECA) on cabbage was also observed. A phenolic substance of unknown structure isolated from cabbage was utilised in a 0.5 mg/ml concentration, p-coumarie acid (PCA) from maize in 0.7 mg/ml concentration and quercetin-glucosyl-coumarate (QGC) found in pea in 8 mg/ml concentration were used. The chosen concentrations were on the limit of their inhibitory effect on the growth of the respective plant apical segments. The effect of DIECA was investigated at 0.1 mg/ml concentration. Using non-labelled L-Try as substrate (5 × 10-3 M) biological tests have shown that QGC and DIECA have a weaker but distinct effect on the increase in auxin level (+ 17% and + 15%, respectively). All phenolic compounds, as well as DIECA increase the intake of L-Try-14C from the incubation solutions. Phenolic substances decrease the conversion of L-Try-14C to its metabolites. The changes were studied after a 20 hour period of L-Try-14C metabolism. On chromatograms changes in the proportion of L-Try-14C metabolites took place, especially in the areas of substances of complex character from which IAA splits off easily,i.e. in the areas of 3-indolylacetylglucose and 3-indolylaeetamide. The radioactivity of these areas is generally decreased (e.g. the decrease of radioactivity in the 3-indolylacetylglucose area under the influence of the phenolic substance ofBrassica plants is -7,4%, under the influence of PCA in maize it is -8.9% and under the influence of QGC in pea it is -17.1% DIECA also decreased the radioactivity of this zone, by -10,5%. In cabbage a large part of L-Try-14C was transformed to glucobrassicin; its biosynthesis increases in the presence of the phenolic substance of cabbage by +3%, in the presence of DIECA by +27%. The results are discussed in a working hypothesis based on the key formation of IAA, accompanied by its oxidation and detoxication.

Water balance of plants during root application of high concentrations of growth substances

V. Kozinka

Biologia plantarum 10:398, 1968 | DOI: 10.1007/BF02920983

Using hydroponic cultures, the effect of high concentrations (10-3 m) of 2-methyl-4-chlorophenoxyacetic acid (MCPA) in the root medium on the water balance of 8-9 week old plants ofPisum sativum L. and of 9-10 week-old plants ofSinapis alba L. was studied. The water balance was determined in the light and in the dark gravimetrically by measuring the intensity of water uptake and transpiration in plants cultivated by the method of root bridges according to Werner. MCPA present in the root medium in illuminated plants decreased rapidly the intensity of both the uptake and the loss components of the water balance. In permanent darkness, MCPA brought about an increase in the intensity of uptake of water and of transpiration. Simultaneous determination of water uptake and transpiration showed that the intensity of transpiration remained higher than the intensity of water uptake. This indicates that in the presence of MCPA in the root medium the relationship between the uptake and the loss components of water balance is not quantitatively equal, enboth in the light and in the dark. The existing disproportion results in the formation of a passive water balance of plants.

Spiral feedback oscillations of growing hypocotyl with radicle inPisum sativum L.

M. Spurný

Biologia plantarum 8:381, 1966 | DOI: 10.1007/BF02930674

Cinematographic records of longitudinal growth showed that hypocotyl with radicle inPisum sativum L. undergoes spiral oscillations during growth. This phenomenon can be characterized by the following time-space limits:
(1) Curvature of the hypocotyl with radicle takes place always in the zone of most rapid elongation (Fig. 2, 8).
(2) Maximum deflections of the root tip from a straight positive geotropic direction of growth correspond to the time when the maximum growth rates were demonstrated (Table 4, Fig. 8).
(3) Shift of zones of maximum elongation proceeds in a spiral path, acropetally to the region of the root tip; as may be seen from the results obtained by studying the growth of roots of perennial (woody) plants this mechanism is preserved throughout ontogeny (Head 1965).
When studying these self-regulating mechanisms the author suggests to use the principles valid for control systems with feedback as a mechanism eliminating errors.

Course of growth and development of Spring Barley

P. Strebeyko, B. Góra

Biologia plantarum 6:152, 1964 | DOI: 10.1007/BF02926670

The barley variety "Browarny P.Z.H.R." was grown in pots. Experiments on the course of development were conducted for two years. The increase in the dry weight of roots, leaves, stems and grain was recorded. The assimilation area of the plants was measured periodically and the rate of respiration in detached leaves was determined. Photographic records were kept of growing point and ear development. The dynamics of growth and development were studied during the whole vegetation period. The present results are similar to those previously obtained for spring wheat. The short period of leaf activity is the chief factor limiting barley growth.

The participation of flavine enzymes in the respiration of root zones of the horse bean (Vicia faba L.).

Věra Hadačová, M. Dvořák

Biologia plantarum 11:450, 1969 | DOI: 10.1007/BF02920709

As part of our studies on respiration systems in root zones of the horse bean (Vicia faba L., cv. Chlumecký) the influence of quininehydrochloride and potassium cyanide on respiration was followed. The inhibition of the oxygen uptake by quininehydrochloride was highest in the elongation zone and lowest in the meristematic zone. Thes shows that the flavine enzyme content is lowest in the meristem, highest in the elongation zone. The inhibition of Fe- and Cu-enzymes by KCN increases from the root tip towards the older parts of the root, but is lower than the inhibition by quininehydrochloride. The difference between the intensity of action of both, applied separately, and of the combined effect of both if compared with the action of cyanide alone, is highest in the elongation zone; this shows that relatively higher amounts of non-metallic flavine enzymes participate in this zone. Their relation to the pentose phosphate cycle is discussed.

Influence of light and darkness on the concentration of lactic, glycolic, succinic, malic and citric acid in pea plants

Jana Barthová, Sylva Leblová

Biologia plantarum 11:97-109, 1969 | DOI: 10.1007/BF02921728

Chromatographic separation of an extract of organic acids on a Dowex-l column in the formiate cycle was used to study the content of several organic acids in pea plants, cultivated either in light or in darkness. Concentration changes of the individual acids in the course of growth indicate that the citrate cycle is blocked in the cotyledons of plants grown in light in the period around the 15th day of growth, probably at the site of succinic dehydrogenase (succinic and lactic acids accumulate and the content of citric and malic acids is exhausted). There is no inhibition in the cotyledons of etiolated plants. In vegetative organs, the concentration of the majority of the acids studied is lower than in cotyledons, probably because synthetic processes prevail over degradation processes in these organs. It seems that other processes besides the citrate cycle participate in malate synthesis in pea plants.

Effect of carbohydrates on the growth of Rhizoctonia solani Kühn

O. P. Israel, Md. Shah Ali

Biologia plantarum 6:84-87, 1964 | DOI: 10.1007/BF02926661

The growth OfRhizoctonia solani in different carbohydrates was studied. The rate of growth of the fungus was traced by taking the dry weights of mycelia obtained from the carbohydrate medium at regular intervals and shifts in the pH were recorded. Different carbohydrate sources had different effects on the growth of the organism. The exoenzymes from the organism were capable of cleaving carbohydrates irrespective of whether the fungus grew in them or not.

Mycoplasma-like bodies in plants infected with potato witches' broom disease and the response of plants to tetracycline treatment

J. Brčák, O. Králík, J. Limberk, Marie Ulrychová

Biologia plantarum 11:470, 1969 | DOI: 10.1007/BF02920712

The virus origin of a Czechoslovak isolate of potato witches' broom disease is discounted: electron micrographs of ultrathin section of phloem tissues from plants infected with potato witches' broom disease demonstratedMycoplasma-like bodies, spherical or elongated showing binary fission and fragmentation. The minute corpuscles have a diameter of about 50-60 nm, the largest bodies of 1000 nm. The width of elongated filamentous structures is about 200 nm, most oval bodies have a diameter of 250 nm. A weak tetracycline treatment of diseased plants causes a delay of symptom development; a strong dose of tetracycline (applied by means of the wick method into the stem) inhibits symptom appearance completely. Tetracycline produces a phytotoxic effect inhibiting the growth of tomato plants and causing (at higher concentrations) necrosis and death of these plants. There is a note in the paper dealing with the term "mycoplasma". The word mycoplasma in the sense ofEriksson (1897) or ofMereschkowsky (1910) does not correspond to the genus nameMycoplasma Nowak (1929).

Effect of trophic conditions on asparagine transamination in wheat plants

Alena Činčerová

Biologia plantarum 11:139, 1969 | DOI: 10.1007/BF02921732

In dialyzed extracts from winter wheat plants transamination reactions occurred between asparagine and α-ketoglutaric acid (L-asparagine+2-oxoacid=2-oxosuccinamate+ +amino acid; 2. 6. 1. 14). Reactions with pyruvate exhibited a very low activity. Besides transamination products,i. e. glutamate and alanine, aspartic acid was formed in both reactions. Deamidation was more intensive in the weak reaction asparagine-alanine and less intensive in the asparagine-glutamate reaction.
When calculated per dry weight unit the activity was the same in plants of all variants (three experimental variants-Knop, potassium humate, water). A higher, activity was found in root dialysates; however, a highly significant difference could be observed only between shoots and roots of Knop variant. When evaluating results in terms of protein content we found a significant difference between mineral variant (Knop-the lowest activity) and both deficient variants (potassium humate, water-the highest activity). Thus the highest growth activity was in connection with the lowest transamination activity and vice versa, which was the same as in transaminations of aspartic acid. In the case of asparagine, too, one can consider the possibility of its utilization via transamination for biosynthesis of glutamic acid in plants which have, for reasons of nutrition, a low level of this metabolically important amino acid.

Glucobrassicin a potential inhibitor of unusual type affecting the Germination and growth of plants; mechanism of its action

Milan Kutáček

Biologia plantarum 6:88-98, 1964 | DOI: 10.1007/BF02926662

A water-soluble germination- and growth-inhibitor present in plants of Brassica genus was studied. Aqueous eluates from chromatograms of methanolic extracts of Savoy cabbage, cabbage and Brussels' sprouts leaves, and of Brussels' sprouts roots, inhibited the growth of wheat; the chromatographic behaviour of this inhibitor was similar to that of the thioglycoside glucobrassicin (GLUBR). GLUBRat 10-3 m, and the supposed product of its enzymatic degradation, 3-indolylacetonitrile (IAN) at 10-4 m, inhibited the growth of wheat and clover in a similar manner. At lower concentrations, both substances gradually enhanced growth. In contrast to IAN, GLUBR did not appreciably influence the growth of Savoy cabbage. Other products of GLUBR degradation, such as thiocyanate ion and ascorbigen, influenced the growth to a lesser extent and in a different way. At higher concentrations GLUBR and IAN also inhibited the germination of clover seeds. The biological effect of GLUBR is dependent on the presence of the indole nucleus in its molecule; the thioglycoside glucoiberin, which is not of indolic character, does not influence growth. The presence of free myrosinase was shown in intact wheat seedlings. Hence, GLUBR taken in by the germinating plants must undergo enzymatic cleavage. The in vitro activity of myrosinase in wheat and clover was lower than in Savoy cabbage, but the quantity of IAN formed at a pH corresponding to the natural pH was higher in wheat and clover than in Savoy cabbage, where the formation of the growth-inactive ascorbigen prevailed. On the basis of these results the hypothesis was formulated that GLUBR, the indolic complex from the generaBrassica, Raphanus, Cochleria, Nasturtium, Barbarea andSisymbrium, is a representative of a class of potential inhibitors of growth and germination, whose activity can be explained by the liberation of large amounts of growth-affecting substances during their specific enzymatic cleavage.

Endogenous auxins in apical buds ofChenopodium rubrum L. after application of (2-chloroethyl) trimethylammonium chloride (CCC)

Lola Teltscherová

Biologia plantarum 11:158, 1969 | DOI: 10.1007/BF02921734

Apical buds ofChenopodium rubrum from plants treated with CCC contain more endogenous auxins than buds from control plants, the level of these compounds increasing with the application of rising concentrations of the retardant. An especially marked increase was observed in the level of substance "X" which on chromatographic separation runs in the zone of tryptamine or its derivative. Since it has been shown in previous experiments that the inhibitory effect of CCC on flowering ofChenopodium rubrum may be reversed by indole-3-acetic acid (IAA) it is believed that the increase in auxins after application of CCC does not concern biologically active substances immediately available to the plant. It seems more likely that inactive precursors are involved which cannot be converted to the active substance in the presence of CCC, possibly due to blocking of the pertinent enzyme. If we assume that the wheat coleoptile used in the auxin bioassay in our experiments contains the pertinent enzyme it might convert the inactive precursors to active substances and, therefore, exhibit a growth stimulation even though the substances concerned would not necessarily be active in the buds from which they were extracted.

Influence of sodium humate and nutritive conditions on the content of nucleic acids, particularly on the ribosomal ribonucleic acid in wheat roots

Svatava Fialová

Biologia plantarum 11:8, 1969 | DOI: 10.1007/BF02920726

Changes in the nucleic acid (NA) content were studied in roots of young wheat plants cultivated under various nutritive conditions, namely in a nutrient solution, in distilled water and in a solution of sodium humate in distilled water. Changes in the ribosomal ribonucleic acid (RNA) in particular and their connection with growth rate were investigated. The amount of the NA fraction investigated (more than 90 per cent of which is represented by the ribosomal RNA) changed substantially under the cultivation conditions studied. In roots of one plant cultivated in water the content of the NA fraction investigated was at the most about 25 μg and it began to decrease as early as from the second day of cultivation. After 12 days of cultivation it decreased to 15 μg. When cultivated in Na-humate the roots contained at the most 33 μg NA, between the 5th-7th day, followed by an intensive decrease after 12 days, to 17 μg. The content of the fraction investigated in wheat plants cultivated in a nutrient solution was about double the value in comparison to these two cases with the maximal value about 60 μg between the 7th-9th day. After 12 days this amount decreased to 45 μg.
The ratio between the growth rate and the NA content presented positive values only when plants were cultivated in a nutrient solution and in Na-humate until the day when the NA content ceased to increase. In the case of Na-humate this took place on the 6th day and in the case of a nutrient solution on the 9th day. Under conditions favourable for growth (in a nutrient solution and in field conditions) the precultivation of wheat plants in Na-humate resulted in a more intensive growth of roots in comparison with the root growth of plants precultivated in distilled water. In plants precultivated in distilled water for 4 days the growth rate continuously increased under favourable nutrition conditions in contrast to plants transferred from distilled water as late as on the 6th day. Their growth stopped after the transplantation and was restored only after a 2 day lag phase. On the other hand, in plants precultivated in Na-humate the transfer to favourable nutritive conditions resulted in both cases in a short term cessation of growth.

Effects of shoot bending of apple trees on accumulation and translocation of14C-labelled assimilates

A. Mika

Biologia plantarum 11:175, 1969 | DOI: 10.1007/BF02921736

The translocation of14C-labelled assimilates from a single leaf in bent and intact apple shoots was studied in varying stages of shoot development. In actively growing shoots14C-labelled assimilates translocated from the treated leaf and accumulated mainly in the shoot apex. In moderately growing apple shoots radioactive assimilates were translocated from the treated leaf in both directions towards and down the shoot. In apple shoots showing only slight growth activity the14C-labelled assimilates were transported from the treated leaf mainly to the base of the shoot, stem and roots. Bending shoots changed the pattern of distribution of radioactive assimilates. Bending actively and moderately growing shoots resulted in higher concentration of 14-carbon in the shoot apex than in controls. In slowly growing and non-growing apple shoots bending caused a higher accumulation of radioactive assimilates in the bent section than in an equal section of control shoots.

Metabolism of nucleic acids in wheat roots in dependence on nutritive conditions

Svatava Fialová

Biologia plantarum 11:424, 1969 | DOI: 10.1007/BF02920706

Influence on individual types (fractions) of nucleic acids (NA) was studied in roots of wheat plants grown in various cultivation media, namely in distilled water, in sodium humate and in a nutrient solution. NA's were prepared by means of the phenol technique. Using separation on a methylalbumine column (MAK) five fractions were obtained, namely: fraction I.- low molecular weight substances, fraction II.-soluble RNA, fraction III.-DNA-RNA, and the ribosomal RNA in two fractions, IV.-(l r-RNA) and V.-(h r-RNA). Of the NA fractions investigated, the r-RNA fraction was noticeably influenced by the kind of nutrition, its amount varying in a certain proportion to the growth intensity affected by the cultivation medium. The other NA fractions were not apparently affected.
The metabolical turnover of the individual NA types (as observed from the specific32P activity) was considerably influenced by the kind of nutrition as well. The specific32P activity in all NA fractions of wheat roots cultivated in a nutrient solution was approximately double that in roots of wheat plants cultivated in distilled water and Na-humate.
Changes in the specific32P activity of r-RNA were again considerably evident. With regard to root growth their relative values appeared in an inverse proportion to the changes in the r-RNA content. The specific32P activity decreased with increasing growth intensity.
Besides changes in the r-RNA fraction, changes in fraction I. were apparent. An especially striking decrease in the specific32P activity was found in roots of plants grown in H2O, namely by about one order in comparison with its specific activity in fraction I. from roots of plants grown in Na-humate and in a nutrient solution.

Integrant characteristics in the deflexion of biserial buds in pea seedlings

R. Dostál

Biologia plantarum 11:193, 1969 | DOI: 10.1007/BF02920797

The main epicotyledonary axis inPisum sativum exhibits a very fluctuating either left- or right-handed deflexion of its first-order laterals, but the biserial buds on the laterals of various further order reveal in this respect certain regularities according to their position on the seedling, their deflexion being very often adaxial in the vicinity of cotyledons, however, in more distant and therefore later produced laterals, as a rule, abaxial. The integrant characteristics of these relationships may become clear from their experimental shift with the aid of exogenous auxin increasing inhibitions as well as of triiodobenzoic acid blocking the transport of endogenous auxin participant also in these embryonic-growth correlations.

Effect of different constant soil moisture levels on foliage development in maize

J. Václavík

Biologia plantarum 11:68, 1969 | DOI: 10.1007/BF02920732

The effects of constant soil moisture levels of 90, 60 and 40% of the maximum capillary capacity, applied beginning from the planting of the germinated kernels, on the dynamics of the foliage development of maize was studied in relation to ontogenical changes in leaf area of individual leaves. There were two maxima in the growth of total leaf area (characterized by leaf area duration and loaf area relative growth rate) unrelated to the soil mositure. The first maximum which was less marked, appeared in the phase of 5-8 leaves, the second and main increase being observed before flowering in the phase of 10-12 leaves. The effects of continued decrease in soil moisture were a correspondingly progressive reduction in leaf area and delayed development of the whole plants. Plants with 60% soil moisture attained the same leaf area as those with 90% but only after the end of the vegetative phase. The greatest differences in rate of development between watering treatments appeared at the time of greatest increase in leaf area of control plants. Continued decrease in soil moisture markedly affected the development of the leaves at different insertion levels (especially in elder leaves). Clear-cut prolongation of ontogenesis took place under dry conditions. In conditions of higher soil moisture growth was rather limited or stopped after reaching a certain maximum.

Cytogenetic studies on the effect of chronic gamma irradiation onVicia faba

Soheir M. Amer, I. Y. Mostafa, Evon Mikhael

Biologia plantarum 11:432-441, 1969 | DOI: 10.1007/BF02920707

Vicia faba seeds (cv. Giza 1) were planted in the Inshas gamma radiation field where they were chronically irradiated during the whole life of the plant. The percentage of the induced abnormal P.M.Cs, as well as the frequency of abnormal P.M.Cs in the different meiotic stages were proportional with the given doses. The main types of chromosome aberrations were anaphase and telophase bridges, fragmentation and lagging chromosomes. The nearest plants to the source showed an inhibition of shoot growth, flower and seed sterility and irregular branching. At the dosage levels used irradiation had no effect on pollen fertility.
Seeds of the 1st filial generation were used for both mitotic and meiotic studies. The percentage of the mitotic abnormalities was proportional with the doses. The most dominant type of anomaly was the presence of micronuclei in the different stages of mitosis and in the resting cells. Irradiation affected also other types of anomaliese.g. lagging chromosomes, fragments, bridges...etc.
Meiosis, and pollen fertility (2nd generation) were normal.

Study of the effect of volatile substances from cereal roots

F. Plhák, Věra Urbánková

Biologia plantarum 11:226, 1969 | DOI: 10.1007/BF02920801

We traced the liberation and biological effect of volatile substances released from the roots of cereals,i. e. barley, wheat, rye and oats, on seedlings of the same and other plant species. Experiments were carried out in a closed glass apparatus with a static or circulating atmosphere in which the CO2 and O2 were permanently absorbed and supplemented, respectively. In some experiments the air was bubbled through water or through solutions of boric acid, barium hydroxide and potassium permanganate.
The roots of all four cereals tested released volatile substances with a biological activity which appeared to be non-specific with respect to plant species. The effect of volatile substances was partially decreased by bubbling through water, barium hydroxide and boric acid and was completely removed after passing through the solution of potassium permanganate.
Volatile substances liberated from roots of barley inhibited elongation of roots and coleoptile, decreased SH-group content and caused excessive formation of root hairs as well as inhibition of both dry matter production and respiration of roots of rye seedlings.
Ethylene was found in the atmosphere of experimental vessels.

Study of the interactions between cereals with respect to the soil nitrate nitrogen

Hana Hruąková, F. Plhák

Biologia plantarum 11:347-355, 1969 | DOI: 10.1007/BF02921399

The interactions between cereals-wheat, barley, rye and oats-in combined cultivation in mixtures of always two species in relation 1:1 in the initial phase of growth were studied. During thirty days' cultivation in pots the growth of the experimental plants in pure cultures and in mixtures and the changes of the nitrate nitrogen content of the soil in the experimental vessels were followed.
The experiments showed that all tested species of cereals interacted with each other during the growth in mixture. The growth changes began soon after sowing, were of a stimulating and inhibiting character and increased during the growth. An exception was the small reaction of oats to rye.
Decrease of the nitrate nitrogen content was determined in the soil of the experimental vessels during the cultivation of the plants. The changes of the nitrate nitrogen in the soil corresponded on the whole with the exception of the last phases of the experiments to the growth intensity of the experimental plants. No essential differences, either in the content or in the rate of the decrease of nitrate nitrogen, were found in the soil of control plants and in the soil of plant mixtures. Small differences which were manifested cannot be considered the primary cause of the mutual relations of cereals. The cause of the mutual relations may be attributed to allelopathic factors.

Growth of radish (Raphanus sativus) seedlings in magnetic fields

M. H. Simmers

Biologia plantarum 9:377-382, 1967 | DOI: 10.1007/BF02929781

Radish seedlings were grown in asymmetric magnetic fields. The number of seeds germinated and the dry weight of the plants were the two criteria by which possible effects were examined. Two experiments were done; in the first the plants were grown for an average of 7.7 days and in the second for 14.1 days. A statistical analysis of the results failed to reveal any significant difference between control plants grown in dummy magnets and those subjected to the magnetic influence.

The effect of 5-azacytidine on the root meristem ofVicia faba

V. Fučík, Z. ©ormová, F. ©orm

Biologia plantarum 7:58-64, 1965 | DOI: 10.1007/BF02920680

The primary roots ofVicia faba seedlings were placed in a solution of 5-azacytidine and their further growth was observed after being replaced in running tap water. No inhibition of elongation occurred during the action of the 10-5 M solution of 5-azacytidine for 24 hours, but during subsequent cultivation in water in the absence of inhibitor, further growth was blocked. This inhibition could be overcome by cytidine, uridine, sodium azide, 5-azidomethyluracil and simultaneously with the 5-azacytidine solution. Inhibition was accompanied by a high incidence of chromosome stickiness and to a less extent by an incidence of chromosome aberrations. The occurrence of stickiness and chromosome aberrations was prevented by adding excess cytidine to the 5-azacytidine solution.

The influence of light, darkness and changes in co2 and o2 concentration in die atmosphere on growth and gas exchange in pea (Pisum sativum)

Jana Barthová, Sylva Leblová, J. Koątíř

Biologia plantarum 9:173, 1967 | DOI: 10.1007/BF02929735

When compared with the control plants, the increase in dry weight in the growing parts of germinating plants ofPisum sativum L., cultivated in a closed atmosphere in darkness or light is heavily inhibited. The concentration of carbon dioxide in the closed atmosphere increased in 60 mg CO2/per 1 g of the final dry weight of plants after 10 days. Similar results were obtained with older plants under similar conditions. The Knop solution caused a slight increase of dry weight in the growing parts of plants, but did not change considerably the relations in gas exchange. The results show that even plants which are able to photosynthetize, are only transpiring under the above conditions; first, when the plants were devoid of cotyledons, as storage organs, the changes in their dry weight (but not the changes in the CO2 and O2 exchange in a closed atmosphere) seemed to show photosynthetic income of CO2 (while its concentration increased to 3-6 volume %) when compared with the control.
From the above results follows that quantative relations between photosynthesis respiration cannot be solved by analysing the gas exchange in a closed atmosphere.

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