biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

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Results 571 to 600 of 608:

A study of the embryo dormancy of Taxus Baccata L. by Embryo Culture

Marie-Theeèse Le Page-Degivey

Biologia plantarum 15:264-269, 1973 | DOI: 10.1007/BF02922707

Mature seeds ofTaxus baccata L. andTaxus baccata L.fastigiata Loudon contain embryos which are both immature and dormant. Embryo cultures were carried out in order to test the relative effciency of gibberellins (GA3 and GA4), chilling and leaching. The increase of germination percentages depends on the treatment applied and the tested variety. WithTaxus baccata, the germination of which is very difficult, a double treatment either leaching and gibberellins or leaching and chilling are necessary to obtain a high percentage of germination. Our results are compared with facts already known on mature dormant embryos of other plants.

Physiology of the cold-resistance of maize during Germination. the reaction of maize (Zea mays L.) to low temperature during germination and its cold-resistance

Vladimír Sege»a

Biologia plantarum 6:189, 1964 | DOI: 10.1007/BF02926677

Attempts to elucidate the physiological basis of cold-resistance during germination led to the investigation of the germination of seeds at low temperature, the effect of pathogenic and parasitic microorganisms being excluded.
At the various experimental temperatures (4°, 6°, 8°, 10° and 14°C) it was found that seed samples of different varieties of maize respond to conditions close to the temperature minimum for germination in three quantitatively different ways. Firstly, caryopses germinate considerably later than at higher temperatures, secondly, the kernels will not germinate even after a prolonged period, without losing their viability and, thirdly, in some of the caryopses the embryos perish.
The lower the temperature used the greater the inhibition of germination and the higher the mortality of the embryos. The increase in mortality with decreasing temperature indicates that the mortality is primarily due to the actual effect of the low temperature. Samples of seeds displayed quantitative difference in the above mentioned response.
Only when fungicide-treated seed material was used for the cold-resistance experiments was a significant relationship found to exist between this property and the general germinating capacity or mortality at the same low temperature.

Changes in the composition of winter rape oil during seed maturation

Ivo Zeman, Vladimír Kratochvíl

Biologia plantarum 9:1-14, 1967 | DOI: 10.1007/BF02930729

The course of biosynthesis of fatty acids in the seeds of winter rape (Brassica napus L. ssp.oleifera, f.biennis cv. Tøebíèská) was investigated. After the termination of flowering seed samples were taken at five intervals, the seeds were divided into 4 fractions according to size, and their weight, water content, oil content and fatty acid composition were determined. The oil content was found to increase in all size categories with time, with the exception of a minute drop when complete maturity is reached. Larger seeds contained more oil. The fatty acid composition changes with time in the individual size fractions almost continuously. The same holds for differences between seed sizes of the same sample. The main change in oil composition consists in the decrease of C18 acids in favour of C22 acids. Greatest decrements during maturation were found with oleic acid, less with linoleic acid. In absolute amounts the quantity of all synthesized acids rises, the greatest rise being observed with C22 acids (i.e. predominantly erucic acid). It follows from the mean rates of synthesis of the individual groups (C16, C18, C20, C22) of fatty acids that the fraction of C22 rate of synthesis increases, while that of the C18 acids decreases with the same speed. The results indicate that the fatty acid synthesis is most intense during the second half of seed maturation, the main role being played by accelerating the synthesis of higher acids, especially of erucic acid.

Distribution of the protein "phaseolin" in some representatives ofViciaceae

Eva Klozová, J. Kloz

Biologia plantarum 14:379-384, 1972 | DOI: 10.1007/BF02933194

Using immunochemical methods the authors investigated the evolutionary taxonomic distribution of the reserve seed protein "phaseolin" in cultivars ofPhaseolus vulgaris, in a series of species ofPhaseolus, and in representatives of some additional genera ofViciaceae.
"Phaseolin" is typical of the seed ofPhaseolus vulgaris L.: it was detected in all 658 investigated cultivars - and also in species related toPhaseolus vulgaris L.(Ph. vulgaris L. ssp.aborigineus Burk.,Ph. polyanthus Green,Ph. dumosus Macf.,Ph. coccineus L., and in an undescribed species from the group ofPh. vulgaris L. -Ph. coccineus L.). A protein immunochemically somewhat similar to "phaseolin" occurs inPh. acufifolius A. Gray. In all other taxa"phaseolin" is absent.

Storage of ethyl methanesulphonate-treated barley seeds with 15 per cent moisture. Influence of treatment and washing conditions

T. Gichner, T. Omura

Biologia plantarum 14:155-163, 1972 | DOI: 10.1007/BF02920960

Barley seeds were treated with ethyl methanesulphonate (EMS), washed for 24 h, redried to 15 per cent moisture and stored at 25°C. The criteria used for expressing the effect of storage were chromosomal aberrations in root tips, M1 germination, M1 seedling height, M1 survival and the frequency of M2 chlorophyll mutants. The increase of the M1 biological injury due to storage was not influenced:
a) by applying EMS solutions at pH 2, pH 7 and pH 10,
b) by lowering the EMS concentration and increasing the treatment time,
c) by different variations of washing with water and by washings with 0.005 N NaOH, 200 mM cysteine or 200 mM thiourea. The rate of the increase of the M1 injury due to storage depends on the EMS dose. With a decrease in the EMS dose the storage effect is more delayed.

On diurnal mitotic activity in root tip meristem ofPicea abies (L.)Karst

T. Terasmaa

Biologia plantarum 14:219-222, 1972 | DOI: 10.1007/BF02921253

The determination of mitotic frequency was made in two variants: one part of seeds was germinated in darkness, the other-under periodical artificial lighting. The diurnal mitotic activity varied from 6.2 to 13.5 per cent. After germination under periodical lighting two peaks of mitotic activity [at 6°c a.m. and 4°c p.m.] were found whereas after germination in darkness there was only one [at 12°c a.m. or 6°c p.m. in different repeats].

Photosensibility of pinus banksiana LAMB. Seeds

Monique Orlandini, Camille Bulard

Biologia plantarum 14:260-268, 1972 | DOI: 10.1007/BF02935850

Germination ofPinus banksiana seeds is controlled by the photoreversible phytochrome reaction. The seeds, even unimbibed, are sensitive to red light. At 660 nm, the energy required to promote germination to the same order of magnitude is much higher for unimbibed seeds than for the imbibed ones. In both cases it is possible to reverse the effect of a single red light irradiation by applying far red light (730 nm).

Pea growth and ion accumulation at varying seed density

Z. La¹tùvka, J. Mináø

Biologia plantarum 12:167-179, 1970 | DOI: 10.1007/BF02920865

Decreased accumulation of elements, particularly of nitrogen, had in dense stand a negative influence on the plants, in spite of the supply of fresh solution and the control of the concentration of nutrients in the pots. The fresh weight and the dry matter of one plant were reduced substantially with rising stand density (from 5 to 10, 20, 40 and 80 plants per 450 sq. cm); the RGR value, the relative absorption rate IM, the content of all tested elements calculated per one plant, the chlorophyll content in the overground parts of one plant, and the distribution index decrease. Qualitatively the same influence is caused by deficiency of nutrients. Plant dying and self-thinning of the culture occurred in denser stand in the course of cultivation. The root-weight ratio rose with stand densification, particularly at the end of the experiment. The net assimilation rate (NAR) related to chlorophyll dropped with stand density; NAR at density "80" rose slightly from the 22nd day of cultivation and did so at further lower densities. Almost all NAR values lie over the control value at the last measurement. The chlorophyll content in mg g-1 dry matter of the overground parts rose with stand density to density "40" (41% more than at density "5"); afterwards it dropped. The accumulation (in mg g-1 d.m.) of phosphorus ions was higher in the plants from dense cultures compared with density "5", particularly in the roots. The accumulation of potassium was near to the control value ("5"), while that of nitrogen was lower. The utilization quotients of phosphorus and potassium in denser stands were the same or negligibly lower than at density "5". They were a little higher in nitrogen than in the controls. Also the ratio RGR/IM was a little higher than in denser stands. The root-weight ratio, the accumulation of elements in mg g-1 d.m., the chlorophyll content in mg g-1 d.m., NARch, the utilization quotient, and the ratio RGR/IM differed qualitatively by the densification of the culture (with complete mineral nutrition) from the influence of element deficiency (at the same stand density).

Effect of root tip amputation on spiral oscillations of the growing hypocotyl with radicle of the pea (Pisum sativum L.)

M. Spurný

Biologia plantarum 10:98, 1968 | DOI: 10.1007/BF02921024

In order to interpret the mechanism of elongation growth of the hypocotyl with radicle in the first stages of germination of the pea seed (Pisum sativum L.) a cybernetic model utilizing feed-back as a mechanism of correcting the wrong direction of growth was proposed (Spurný 1966, 1967). In the present study, the effect of amputation of the root tip as the control centre on the trajectory of the growing radicle was investigated. The results have shown that the hypocotyl grows, elongates-the rate of growth being slightly lower than that of the standard, but that no spiral oscillations at all are executed by the organ after amputation of the root tip. This finding appears to confirm the applicability of the proposed cybernetic model, for amputation of the root tip means that not only the control block is eliminated, but also that the channel of feed-back impulses to zone of elongation is interrupted.

Heat-treatment dependent dormancy in seeds of Psoralea corylifolia Linn.

S. P. Shukla

Biologia plantarum 13:88-99, 1971 | DOI: 10.1007/BF02936825

The intact seeds ofPsoralea corylifolia which are actieually one-seeded fruit, presents a unique problem of dormancy. Germinability of these dormant seeds is not enhanced as a result of a number of treatments: 1) Dry-storage of 44 months under moderate conditions of the laboratory. 2) Periodic germination in different months of the year. 3) Immersion in ethylalcohol (70% and 100%), hydrogen-peroxide, nitric acid, acetone, ether solvent and hydrochloric acid. 4) Removal of pericarp.
Four distinct methods have also been evolved which effectively overcome the dormancy of the seeds: 1) Mechanical puncturing of seedcoverings. 2) Sulphuric acid treatment. 3) High temperature treatment. 4) Weathering of seeds.
Germination-behaviour of the latter 4 lots of seeds is compared and ecological significance of the reported dormancy mechanism is discussed.

Storage of ethyl methanesulphonate-treated barley seeds with low moisture contents

T. Gichner, T. Omura

Biologia plantarum 13:383-390, 1971 | DOI: 10.1007/BF02930928

Barley seeds were treated with ethyl methanesulphonate (EMS) for 3 h at 25° C, washed with tap water for 24 h at 25° C, redried at 40° C to different moisture contents below 15% and stored at 25° C in desiccators or in sealed plastic bags. The criteria used for expressing the effect of storage were the M1 seedling height and the frequency of chromosomal aberrations. With 14.9% seed moisture a strong increase of biological injury occurred in the course of a 2-week storage, while storage of seeds having an initial moisture content of 11.7% led to a significant increase of injury only after 6 weeks. Superdry EMS-treated seeds with 5% or less moisture can be stored at 25° C without any changes in the biological effects. A method is recommended to avoid the EMS-storage effects.

Some growth responses of apple seedlings to abscisic acid and growth stimulators

W. Kamiñski, R. Rudnicki, Janina Pieni±¿ek

Biologia plantarum 13:128-132, 1971 | DOI: 10.1007/BF02936829

Antonovka seedlings were obtained from the embryos soaked in abscisic acid (ABA) alone, gibberellin A4+7, benzyladenine or the mixtures of these regulators. The inhibitory effect of ABA on the growth of the seedlings was of temporary duration. The height of the 12 week-old seedlings was dependent only on the degree of seed after-ripening and not on the ABA treatment.
The growth stimulators studied were unable to overcome the inhibitory effect of ABA observed in the early stages of seedling growth. The growth stimulators did not substitute, for cold treatment of apple seeds which is the only treatment so far known to overcome the dwarf conditions of the seedlings obtained from non-after-ripened embryos. ABA greatly suppressed the early stages of apple seedling growth (up to 6 weeks) during which they resembled physiological dwarfs.
The highest concentrations of ABA (2.0 μg ml-1) greatly modified the root system of the seedlings, and produced a larger percentage of seedlings with unbranched roots.

Heat-treatment dependent dormancy in seeds of Psoralea corylifolia Linn.

S. P. Shukla

Biologia plantarum 13:88-99, 1971 | DOI: 10.1007/BF02930752

The intact seeds ofPsoralea corylifolia which are actieually one-seeded fruit, presents a unique problem of dormancy. Germinability of these dormant seeds is not enhanced as a result of a number of treatments: 1) Dry-storage of 44 months under moderate conditions of the laboratory. 2) Periodic germination in different months of the year. 3) Immersion in ethylalcohol (70% and 100%), hydrogen-peroxide, nitric acid, acetone, ether solvent and hydrochloric acid. 4) Removal of pericarp.
Four distinct methods have also been evolved which effectively overcome the dormancy of the seeds: 1) Mechanical puncturing of seedcoverings. 2) Sulphuric acid treatment. 3) High temperature treatment. 4) Weathering of seeds.
Germination-behaviour of the latter 4 lots of seeds is compared and ecological significance of the reported dormancy mechanism is discussed.

Changes in content of sugars and their exosmose from maize kernels in relation to cold resistance

Eliska Vedralová, V. Sege»a

Biologia plantarum 12:265-274, 1970 | DOI: 10.1007/BF02920811

Changes in soluble sugar content in individual parts (embryo, endosperm and pericarp) of maize caryopses exposed to the influence of 24°, 10° and 6° were studied. At the same time the eluates from sand in which the seeds were planted and exposed for various periods were analysed by paper chromatography.
It was found that the lower was the temperature of exposure the greater were the amounts of sucrose, glucose, raffinose and other sugars diffused from the caryopses into the medium before the start of germination. The exosmose course and the changes in sugar content in kernel tissues proved that the hydrolysis of storage polysaccharides is not inhibited by low temperatures but that under such conditions the use of products of hydrolysis for the growth of the embryo is inhibited.
It is concluded that not only surface parts of endosperm but also the embryo participates in exosmose. The relatively high sugar exosmose found from seeds germinated at low temperatures explains the well-known interaction of pathogenic and parasitic microorganism in cold resistance during emergence.

Content of free amino acids and their exosmose from maize kernels in relation to cold resistance

V. Sege»a, Eli¹ka Vedralová

Biologia plantarum 12:315-326, 1970 | DOI: 10.1007/BF02922185

Maize caryopses were exposed in moist, sterile sand to temperatures of 24°, 10° and 6°. The amino acid content of embryo, endosperm and pericarp tissues of kernels and amino acid occurence in sand eluates was analysed by paper chromatography.
The lower the temperature to which the seeds were exposed and the greater the delay in start of germination, the greater were the amounts of amino acids exosmosed from the kernel to the surrounding media. The exudation of aspartic acid, proline, alanine, γ-aminobutyric and glutamic acids was especially high.
The time-course of amino acid content in various kernel parts proves that cold does not check the enzymic hydrolysis of reserve nitrogen compounds. There is, however, an inhibition of translocation and use of amino acids in the embryo. Therefore, amino acids diffuse out from the caryopse into the media. Although mainly the peripheral parts of endosperm take part in exosmose, participation of the embryo in the exudation of certain amino acids (proline) can be supposed as well.
In cold resistance of emerging maize kernels the remarkable interaction of microorganisms is causally related to the high amino acid exosmose found in these experiments. The mechanism of amino acid exosmose and its relationship to cold resistance are discussed in detail.

Phosphorus metabolism during ripening of Glycine max. (L.)Merril

S. K. Arora, B. M. Gandhi

Biologia plantarum 12:139-143, 1970 | DOI: 10.1007/BF02920862

The seeds, seed covers and leaves, taken after 17, 27, 37, and 47 days after tagging of flowers of soybean, were analysed quantitatively for their contents of different phosphorus fractions. Total phosphorus content increased in seed cover and leaves, there was a gradual decrease during ripening. All the phosphorus fractions i.e. acid soluble-P, lipid-P, nucleic acid-P, and protein-P were found to increase with maturity in seeds whereas in case of seed covers the content of acid soluble-P, nucleic acid-P and protein-P decreased but a marked increase was observed in lipid-P. In leaves during ripening, all the phosphorus fractions decreased except protein-P which was found to be almost constant. In lipid-P an increase was observed during later stages of maturity.

The effect of dinitrophenol, sodium arsenate, sodium phosphate and indole-3-acetic acid on the heat of respiration of germinating wheat seeds

S. J. Maleszewski

Biologia plantarum 12:58-63, 1970 | DOI: 10.1007/BF02920879

The rate of carbon dioxide evolution, respiration quotient, and the rate of liberation of heat were determined on the 3rd day of germination of wheat seeds. The seeds were germinated with pure water (control) or they were transferred for a 5-hour period to a solution of dinitrophenol, sodium arsenate, sodium phosphate or indole-3-acetic acid. The heat liberated was compared with the total energy released in respiratory processes of the seedlings, calculated from the gas exchange measurements. The amount of heat liberated for 1mm of carbon dioxide evolved and for 1mm of oxygen absorbed was definitely changed in the presence of the compounds used. However, the ratio of heat liberated to the total energy released in respiration of the seedlings changed only slightly.

Glucobrassicin a potential inhibitor of unusual type affecting the Germination and growth of plants; mechanism of its action

Milan Kutáèek

Biologia plantarum 6:88-98, 1964 | DOI: 10.1007/BF02926662

A water-soluble germination- and growth-inhibitor present in plants of Brassica genus was studied. Aqueous eluates from chromatograms of methanolic extracts of Savoy cabbage, cabbage and Brussels' sprouts leaves, and of Brussels' sprouts roots, inhibited the growth of wheat; the chromatographic behaviour of this inhibitor was similar to that of the thioglycoside glucobrassicin (GLUBR). GLUBRat 10-3 m, and the supposed product of its enzymatic degradation, 3-indolylacetonitrile (IAN) at 10-4 m, inhibited the growth of wheat and clover in a similar manner. At lower concentrations, both substances gradually enhanced growth. In contrast to IAN, GLUBR did not appreciably influence the growth of Savoy cabbage. Other products of GLUBR degradation, such as thiocyanate ion and ascorbigen, influenced the growth to a lesser extent and in a different way. At higher concentrations GLUBR and IAN also inhibited the germination of clover seeds. The biological effect of GLUBR is dependent on the presence of the indole nucleus in its molecule; the thioglycoside glucoiberin, which is not of indolic character, does not influence growth. The presence of free myrosinase was shown in intact wheat seedlings. Hence, GLUBR taken in by the germinating plants must undergo enzymatic cleavage. The in vitro activity of myrosinase in wheat and clover was lower than in Savoy cabbage, but the quantity of IAN formed at a pH corresponding to the natural pH was higher in wheat and clover than in Savoy cabbage, where the formation of the growth-inactive ascorbigen prevailed. On the basis of these results the hypothesis was formulated that GLUBR, the indolic complex from the generaBrassica, Raphanus, Cochleria, Nasturtium, Barbarea andSisymbrium, is a representative of a class of potential inhibitors of growth and germination, whose activity can be explained by the liberation of large amounts of growth-affecting substances during their specific enzymatic cleavage.

Qualitative differences of pumpkin (Cucurbita pepo L.) proteins influenced by Ca2+ nutrition

M. Dvoøák, Jana Èernohorská

Biologia plantarum 11:388, 1969 | DOI: 10.1007/BF02921406

The effect of Ca-deficiency on changes in the protein content of cotyledons and roots of germinating pumpkin (Cucurbita pepo L.) plants was studied by means of immunoelectrophoresis. Antiserum against seed proteins gave 11 clear precipitation zones in homological reactions and 7 zones with proteins from cotyledons of 10 day-old plants. Several less mobile zones in the area of start appeared in variant "-Ca". Using antiserum against root proteins of 10 day-old control plants, 8 precipitation zones in the homological reaction and 6 zones (two cathodic zones are missing) in the heterological reaction were obtained. The patterns of proteins from the root and those arising from the protein of seed are both changed under Ca-deficiency.

Occurrence of ethanol in pea plants in the course of growth under normal and anaerobic conditions

Sylva Leblová, Ilona Zimáková, Dana Sofrová, Jana Barthová

Biologia plantarum 11:417, 1969 | DOI: 10.1007/BF02920705

1) At a so-called natural anaerobiosis during the first 48 hours of germination the concentration of ethanol in pea tissues increases (according to the cultivation conditions) up to 40 μmol per gram fresh weight.
2) In a nitrogen atmosphere the content of ethanol in pea seedlings increases as well, and after a 90 hour incubation in N2 it can reach even 100 μmol ethanol per gram fresh weight. In older plants the content increases the most markedly in cotyledons, but considerable amounts were revealed also in stems and roots. Its increase in vegetative organs of plants cultivated both in light and darkness is more or less identical. Ethylalcohol can be formed by the vegetative organs themselves, as proved by the increase of this metabolite in plants deprived of reserve organs; in addition, however, it is evidently transported into them from reserve parts. Ethanol formed under anaerobiosis is catabolyzed after transferring plants to the air.

Cytogenetic studies on the effect of chronic gamma irradiation onVicia faba

Soheir M. Amer, I. Y. Mostafa, Evon Mikhael

Biologia plantarum 11:432-441, 1969 | DOI: 10.1007/BF02920707

Vicia faba seeds (cv. Giza 1) were planted in the Inshas gamma radiation field where they were chronically irradiated during the whole life of the plant. The percentage of the induced abnormal P.M.Cs, as well as the frequency of abnormal P.M.Cs in the different meiotic stages were proportional with the given doses. The main types of chromosome aberrations were anaphase and telophase bridges, fragmentation and lagging chromosomes. The nearest plants to the source showed an inhibition of shoot growth, flower and seed sterility and irregular branching. At the dosage levels used irradiation had no effect on pollen fertility.
Seeds of the 1st filial generation were used for both mitotic and meiotic studies. The percentage of the mitotic abnormalities was proportional with the doses. The most dominant type of anomaly was the presence of micronuclei in the different stages of mitosis and in the resting cells. Irradiation affected also other types of anomaliese.g. lagging chromosomes, fragments, bridges...etc.
Meiosis, and pollen fertility (2nd generation) were normal.

Seed failure following hybridisation between 4nCorchorus capsularis L. (strain D. 154) and 3nC. olitorius L. (strain C.G.) and its reciprocal and some cytogenetical observations

R. M. Datta, M. R. Choudhury

Biologia plantarum 8:288-291, 1966 | DOI: 10.1007/BF02930664

In both types of crosses (3n C.G.×4n D 154 and 4n D 154×3n C.G.) pods were found to set. In the first case pods shed within 4 days. In the second case pods were found to persist till maturity. Only a few uppermost ovules were found to be fertilized. Pollen tubes were seen to reach the ovules within 24 hours.
In 4n D 154×3n C.G. fusion of sperm with the egg was not noted to be complete till 3 days but the secondary nucleus is fertilized by other sperm. In 3n C.G.×D 154 fusion of sperm with egg or with polars was not complete within 3 days. Signs of degeneration of ovules were noted on the 5th day in both cases.

The action of 1-alkyl-1-nitrosoureas and 1-alkyl-3-nitro-1-nitrosoguanidines on the M1 generation of barley andArabidopsis thaliana (L.) heynh.

Jiøí Velemínský, Tomá¹ Giohner, Václav Pokorný

Biologia plantarum 9:249, 1967 | DOI: 10.1007/BF02929765

The activity of 1-methyl-1-nitrosourea (MNH), 1-ethyl-1-nitrosourea (ENH), 1-methyl-3-nitro-1-nitrosoguanidine (MNG) and 1-ethyl-3-nitro-1-nitrosoguanidine (ENG) was tested on seeds of barley andArabidopsis. The activity of nitrosoamides tested was expressed by the germination and M1 seedling height reduction of barley and M1 root length reduction ofArabidopsis.
1) After the action of both nitrosoureas (MNH and ENH) the germination of barley is at the same level as that of controls, even at concentrations, leading to a maximal reduction in the height of seedlings. After the action of both nitrosoguanidines (MNG and ENG) germination decreases in parallel with the decreasing seedling height. InArabidopsis no such differences in the relation germination to root length reduction were observed after nitrosoureas and nitrosoguanidines treatment. The differences in the M1 generation of barley andArabidopsis after nitrosoguanidines treatment may be the reason for the non-mutagenic action of MNG and ENG in barley.
2) All 4 tested nitrosoamides are active between pH 3 and pH 6 and almost entirely ineffective at pH 7 and in basic solutions.
3) The effectiveness (in relation to M1 seedling height reduction) of the tested nitrosoamides with a methyl group (MNG and MNH) is higher than of the nitrosoamides with an ethyl group (ENG and ENH).
4) 50% decomposition of aqueous solutions (at 25° C) of MNH and ENH takes about 60 hours, for MNG 120 hours and for ENG 420 hours.
5) Aeration during treatment or a period in the cold (5° C) after treatment improved the germination of MNG treated seeds. Sucrose, KNO3 and thiourea had no effect.

On the lateral growth correlations exemplified by petioles and axillaries ofPrisum cotyledons

R. Dostál

Biologia plantarum 9:330, 1967 | DOI: 10.1007/BF02929775

The cotyledonary petioles inPisum sativum developing at the start of germination, as well as the cotylaries growing out after decapitation of thePisuin seedlings manifest very often the so-called lateral correlations, since one of them suppresses the opposite primordium when the growth inhibition in the plant coinciding apparently with the growth promotion results necessarily in a normal development only of one of these originally equal primordia. These correlations also seem to be mediated by auxin, being induced by gravity or by corresponding cotyledon and root products, which can be supported by the use of exogenous auxins.

Effect of sodium humate on swelling and Germination of winter wheat

Miroslava ©mídová

Biologia plantarum 4:112-118, 1962 | DOI: 10.1007/BF02932931

Experiments on the effect of sodium humate on the swelling and germination of the winter wheat Py¹elka (Triticum vulgare Vill.) and on the changes in respiration intensity of swelling seeds during the first 24 hours of swelling, are described.
It was found that sodium humate at a concentration of 100 mg./l. accelerates the uptake of water by swelling seeds during the initial phase of swelling. The fact that the seeds take up a sufficient amount of water sooner makes it possible for the activation of enzyme systems which ensure normal germination to take place, thereby bringing about an increase in respiration intensity. The energy released during respiration is then utilized for more rapid growth of the embryo which is morphologically reflected in the rate of germination.

Carbon metabolism and morphogenesis of Penicillium frequentans: Glucose consumption

K. B. Deshpande, B. D. Saeje

Biologia plantarum 8:29, 1966 | DOI: 10.1007/BF02971552

The paper deals with the relationship between the glucose utilization and growth ofPenicillium frequentans. The vegetative growth was scanty and sporulation started at a time when glucose was abundant in the culture medium. Occurrence of autolysis in spite of the presence of glucose in the culture medium is a peculiar phenomenon reported here. The autolysing culture gives rise to conditions which favour the germination of spores and the development of vegetative growth. The citric acid is the major metabolite produced by the fungus and its biochemical origin appears to follow condensation of oxalacetate and acetate. Lastly production of citrmin as another metabolite has also been reported.

Protein characters and relationship between Phaseolus vulgaris ssp. Aborigineus Burk. and related taxons of the genus Phaseolus

Josef Kloz, Eva Klozová, Vìra Turková

Biologia plantarum 8:187-196, 1966 | DOI: 10.1007/BF02930777

Both quantitative and qualitative immunochemical methods were used for studying the mutual relationships of several spocies and the subspecies of the genusPhaseolus: Ph. vulgaris L. ssp.vulgaris, Ph. vulgaris L. ssp.aborigineus Burk.,Ph. coccineus L.,Ph. acutifolius A. Gray,Ph. lunatus L. (American endemites) andPh. aureus L. (a typical Asian bean). Protein characters of cotyledons (i.e., "storage" proteins) of the above species were compared with the aid of antisera prepared against seed (cotyledon) proteins ofPh. vulgaris L. ssp.vulgaris, cv. Veltruská Saxa, using
(a) the whole complex of cotyledon protein,
(b) the albumin fraction of this complex,
(c) the globulin fraction,
(d) crystaline phaseolin.
Our results are in agreement with the morphological and genetic data ofBurkart andBrücher on the close relationship betweenPh. vulgaris L. ssp.vulgaris andPh. vulgaris L. ssp.aborigineus (both contain a character designated asPh. vulgaris protein 1 which is lacking in the others, both contain an identical phaseolin and exhibit only negligible differences in the specificity of proteins). The closest to these two species isPh. coccineus (almost identical phaseolin, small differences in the albumin and globulin fractions, a greater quantitative difference in protein specificity).Ph. acutifolius contains a somewhat different phaseolin, exhibits greater deviations in the albumin and globulin fractions and a greater quantitative difference in protein specificity.Ph. lunatus andPh. aureus are quite different in all the above respects. These data are in good agreement with genetic data (crossability).

Studies on energy metabolism of germinating wheat seeds

S. J. Maleszewski

Biologia plantarum 7:31, 1965 | DOI: 10.1007/BF02920677

The amount of energy dissipated in the form of heat in the metabolic processes carried on during the initial period of the germination of wheat seeds was determined directy by means of an adiabatic microcalorimeter. The total energy simultaneously liberated in the respiration of the investigated seeds was calculated from the carbon-dioxide production. The proportion of total energy released in respiration to the amount of energy dissipated as heat indicates that about 5 per cent of respiratory energy is retained in the tissues of growing seedlings. The percentual rate of energy dissipated is not dependent on the temperature or the age of seedlings.

Etude microcalorimétrique de l'accélération de la germination des graines, traitées par les ultra-sons

George Obolensky

Biologia plantarum 2:227-239, 1960 | DOI: 10.1007/BF02920652

On a traité des graines d'orge à différentes fréquences ultrasonores (30, 80, 570, 720, 960 kHz) avec des puissances de 2 à 5 W/cm2, de 5 à 25 minutes, en vue d'étudier le mécanisme d'action des ultra-sons sur le processus de la germination.
A cet effet, nous avons effectué des enregistrements microcalorimétriques au laboratoire de Monsieur le ProfesseurE. Calvet à la Faculté des Sciences de Marseille, en vue de comparer les courbes représentant la cinétique de la thermogénèse dans le cas des graines traitées ou non traitées par les ultra-sons.
Les courbes montrent que la durée de la thermogénèse physico-chimique caractérisée par la première pointe exothermique est diminuée dans le cas des graines traitées et que le phénomène passe alors rapidement de la phase physico-chimique à la phase de thermogénèse biologique, la deuxième phase pouvant même se superposer à la phase physico-chimique.
Ainsi le traitement provoque une accélération de la germination et modifie favorablement la durée de celle-ci (thermogénèse biologique plus active). Le phénomène d'accélération de la germination se manifestant de la même manière sur des graines dont la thermogénèse fut étudiée plusieurs semaines et plusieurs mois après le traitement aux ultra-sons, cela montre que cette action est irréversible et correspond principalement à une modification de la perméabilité des membranes. Il est cependant possible qu'il vienne s'y associer des actions dépolymérisantes (Prat, Calvet 1945) (dépolymérisation de l'amidon en dextrine) qui expliqueraient, mieux que la seule absorption d'eau, la prolongation dans le temps de l'accélération de la croissance (diminution de la phase endothermique et accélération de la phase exothermique).
Ainsi la thermogénèse des graines traitées aux ultra-sons, grâce à la grande précision de la méthode microcalorimétrique, nous montre que la pénétration ultérieure de l'eau dans les graines, ainsi que les processus enzymologiques produisant la thermogénèse biologique, sont nettement accélérés par les ultra-sons.

Studies of some ways in which carrot mosaic virus can be transmitted

J. Chod

Biologia plantarum 7:463-468, 1965 | DOI: 10.1007/BF02920690

The transmission of carrot mosaic virus (CMV) by the crude sap to 11 varieties of plants from 4 families was demonstrated. From these plants the virus could be transferred back to a healthy carrot cultivated from the seed in isolation. The incubation time required for the appearance of the symptoms of CMV was 7-20 days. The plants on which mosaic or spot symptoms appeared on the leaves after transfer by the sap at temperatures below 15°C remains habitually healthy after the transfer of virus at higher temperatures.
The results of the mechanical inoculation of CMV by the crude infectious sap to young carrots cultivated from seeds differentiated this virus fromApium virus 1, which after mechanical inoculation causes chlorosis of the youngest carrot leaves in contrast to CMV. A further differentiation of CMV fromApium virus 1 is shown by the fact that CMV can be transferred only to the familyDaucaceae. It differs in this fromApium virus 1 which is transferred exclusively to this family (Köhler, Klinkowski 1954). CMV is differentiated fromCucumis virus 1/Doolittle Smith by some different host plants.

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